FastQCFastQC Report
Fri 10 Feb 2017
ERR1630569.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630569.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902141
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83230.43755957103074905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64720.3402481729798159No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59560.3131208464567033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43210.22716507346195683No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC23700.12459644158871502No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA20100.1056703998284039No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT19660.10335721694658807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT3150.019.96825436
GGTATCA33950.019.50811
GCTTTAT7600.017.5263161
AAGACGG3250.017.0769235
CGAATTA803.3840217E-416.187515
ACGTTCG1951.8189894E-1216.1282067
TATTGAG7800.015.6538475
TGCGCTA855.3664454E-415.2352945
TATACTG2350.014.9574475
ATGCCGT4250.014.834
GTATAGA2151.2732926E-1114.6279071
ATGCGTT1304.44736E-614.23076836
TGCCGTC4450.014.13483235
CGTTCGA2101.364242E-1014.0952388
CGGACCA3550.014.0704229
CCGTCTT5300.013.96226437
AGACGTT2405.456968E-1213.8750015
CTAGTAC800.006300620313.8750013
AATGCGT1356.5719105E-613.70370435
CAGTCGG4050.013.70370410
Produced by FastQC (version 0.11.5)