##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630569.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1902141
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01500572249901	33.0	31.0	34.0	30.0	34.0
2	32.172760589251794	33.0	31.0	34.0	30.0	34.0
3	32.280144847306275	34.0	31.0	34.0	30.0	34.0
4	35.85760203896557	37.0	35.0	37.0	35.0	37.0
5	35.83404647710133	37.0	35.0	37.0	35.0	37.0
6	35.87334219702956	37.0	35.0	37.0	35.0	37.0
7	35.84707810830007	37.0	35.0	37.0	35.0	37.0
8	35.84666541544502	37.0	35.0	37.0	35.0	37.0
9	37.566860185443666	39.0	37.0	39.0	35.0	39.0
10	37.48337268372849	39.0	37.0	39.0	35.0	39.0
11	37.5872293378882	39.0	37.0	39.0	35.0	39.0
12	37.520493486024435	39.0	37.0	39.0	35.0	39.0
13	37.57044667035724	39.0	37.0	39.0	35.0	39.0
14	38.84589049917961	40.0	38.0	41.0	35.0	41.0
15	38.81916797966081	40.0	38.0	41.0	35.0	41.0
16	38.7951234950511	40.0	38.0	41.0	35.0	41.0
17	38.792960669056605	40.0	38.0	41.0	35.0	41.0
18	38.78792108471454	40.0	38.0	41.0	35.0	41.0
19	38.80961295718877	40.0	38.0	41.0	35.0	41.0
20	38.79276720285194	40.0	38.0	41.0	35.0	41.0
21	38.722649372470286	40.0	38.0	41.0	34.0	41.0
22	38.66764661505115	40.0	38.0	41.0	34.0	41.0
23	38.65232440707603	40.0	38.0	41.0	34.0	41.0
24	38.60167306209161	40.0	38.0	41.0	34.0	41.0
25	38.558442828370765	40.0	38.0	41.0	34.0	41.0
26	38.367476438392316	40.0	38.0	41.0	34.0	41.0
27	38.25089254687218	40.0	38.0	41.0	34.0	41.0
28	38.156620355693924	40.0	38.0	41.0	33.0	41.0
29	38.06347268683026	40.0	37.0	41.0	33.0	41.0
30	37.98217219438517	40.0	37.0	41.0	33.0	41.0
31	37.922606158008264	40.0	37.0	41.0	33.0	41.0
32	37.8380267288282	40.0	37.0	41.0	33.0	41.0
33	37.79012228851594	40.0	37.0	41.0	33.0	41.0
34	37.71580340258687	40.0	37.0	41.0	33.0	41.0
35	37.65450983917596	40.0	37.0	41.0	33.0	41.0
36	37.600801412723875	40.0	37.0	41.0	33.0	41.0
37	37.41709526265403	40.0	36.0	41.0	32.0	41.0
38	37.305476302755686	40.0	36.0	41.0	32.0	41.0
39	37.23306106119367	40.0	36.0	41.0	31.0	41.0
40	37.112434356864185	40.0	36.0	41.0	31.0	41.0
41	36.99120307064513	39.0	36.0	41.0	31.0	41.0
42	36.8917525041519	39.0	35.0	41.0	31.0	41.0
43	36.053394569592896	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	6.0
10	1.0
11	4.0
12	3.0
13	1.0
14	2.0
15	3.0
16	4.0
17	30.0
18	63.0
19	121.0
20	214.0
21	495.0
22	890.0
23	1623.0
24	2637.0
25	4326.0
26	6645.0
27	9748.0
28	14163.0
29	19772.0
30	27227.0
31	35715.0
32	46635.0
33	60413.0
34	80392.0
35	108116.0
36	151113.0
37	233535.0
38	419308.0
39	678933.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.666899562124996	19.629827652103604	12.098577339955344	24.60469544581606
2	19.105260861313646	21.128086719123345	33.88071651891211	25.885935900650896
3	21.106321771098987	23.498047726220086	28.938969298280202	26.456661204400728
4	14.575102476630281	15.324889164367942	34.43661642328303	35.66339193571875
5	14.509019047483863	37.024910351020246	33.91820059606518	14.547870005430722
6	35.02921181973366	34.19567739720662	15.474667755965516	15.300443027094207
7	28.884451783542858	30.332188833530218	21.548770569584484	19.234588813342437
8	27.943459501687833	31.929441613424032	20.223632212333364	19.903466672554767
9	26.577419865299156	13.883881373673141	20.691841456548175	38.846857304479535
10	17.94519964608302	26.65580522158978	32.45753075087494	22.94146438145227
11	35.042670338318764	22.090107936267607	21.505398390550436	21.36182333486319
12	22.488711404675048	25.8262662967677	28.24454128269145	23.440481015865807
13	30.13993179264839	20.08930988817338	25.30006976349282	24.47068855568541
14	22.866653944160817	21.498931992948997	25.5375390152465	30.096875047643685
15	25.811756331418124	27.897511278080856	22.758670361450598	23.532062029050422
16	24.99835711443053	26.413289025366677	24.699693660985176	23.888660199217618
17	23.43049227160342	26.623368088906137	25.78578559633592	24.16035404315453
18	23.1528577534473	25.014969973309025	27.7411611442054	24.09101112903828
19	24.166399862050184	25.395488557367724	27.650473860770575	22.787637719811517
20	24.742697833651658	24.843268716672423	26.906995853619687	23.507037596056232
21	24.713888192305408	25.22830852181831	26.81630857018486	23.241494715691424
22	24.561270694443788	25.319574100973586	26.957570442990292	23.161584761592334
23	23.89607289890707	25.10602526311141	26.79901227090947	24.19888956707205
24	23.654660721786662	25.96963106310205	26.95646642388761	23.41924179122368
25	24.018408729952196	25.71823014171925	26.5549714768779	23.708389651450652
26	23.650928085772822	26.260671527505057	26.82377384221254	23.264626544509582
27	23.983342980357396	25.400903508204703	26.6772547355848	23.938498775853105
28	23.192234434776392	25.829262920046407	27.16828037458842	23.810222270588774
29	23.328186501421293	26.249631336478213	27.267747238506505	23.154434923593993
30	23.52743566328679	26.046281532231312	26.849271426250738	23.577011378231163
31	22.937363739070868	26.72930135042565	26.883390873757516	23.44994403674596
32	22.938835764541114	25.832522405016245	26.920296655190125	24.30834517525252
33	22.472781986193453	26.10043104060109	27.387244163287576	24.039542809917876
34	23.933767265413028	25.386025536487566	26.99521223715802	23.68499496094138
35	23.287810945665964	25.462097709896376	27.329362018903964	23.920729325533703
36	22.652579382916407	26.277126669368883	27.236256407910876	23.83403753980383
37	23.173255820677856	24.967181717864236	27.894094076096355	23.96546838536155
38	23.026631569373667	25.10413265893538	27.907132015975684	23.96210375571527
39	22.957236082919195	24.898206810115546	28.13477024048165	24.00978686648361
40	22.20939457169579	24.85735810331621	28.62895021977866	24.30429710520934
41	21.524377004648972	25.003561775914612	29.03486124319911	24.437199976237302
42	21.934493815127272	24.98184940022848	29.27401280977593	23.80964397486832
43	22.040689938337906	24.559903813649985	28.925983930739097	24.473422317273013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	642.0
1	571.0
2	500.0
3	1025.5
4	1551.0
5	1551.0
6	2118.0
7	2685.0
8	2517.0
9	2349.0
10	3497.5
11	4646.0
12	4646.0
13	8156.0
14	11666.0
15	14996.5
16	18327.0
17	18544.5
18	18762.0
19	18762.0
20	22552.0
21	26342.0
22	24986.5
23	23631.0
24	26926.0
25	30221.0
26	30221.0
27	33827.5
28	37434.0
29	42185.5
30	46937.0
31	52858.0
32	58779.0
33	58779.0
34	66321.5
35	73864.0
36	80689.0
37	87514.0
38	93490.5
39	99467.0
40	99467.0
41	103247.0
42	107027.0
43	108702.0
44	110377.0
45	111550.0
46	112723.0
47	112723.0
48	117977.5
49	123232.0
50	124315.0
51	125398.0
52	122304.5
53	119211.0
54	119211.0
55	117391.0
56	115571.0
57	112380.5
58	109190.0
59	108396.5
60	107603.0
61	107603.0
62	99204.5
63	90806.0
64	82626.5
65	74447.0
66	64464.0
67	54481.0
68	54481.0
69	48523.5
70	42566.0
71	35973.0
72	29380.0
73	22098.5
74	14817.0
75	14817.0
76	11091.0
77	7365.0
78	6015.0
79	4665.0
80	3953.5
81	3242.0
82	3242.0
83	2537.0
84	1832.0
85	1581.5
86	1331.0
87	1159.0
88	987.0
89	987.0
90	734.5
91	482.0
92	273.5
93	65.0
94	40.5
95	16.0
96	16.0
97	12.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1902141.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.43193434820382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.20091291613738	48.932532370066326
2	9.47965577202836	10.888699358854081
3	2.280063438123402	3.9284536106412937
4	0.8847992877490404	2.0326293842136156
5	0.47466635356472175	1.36305034276152
6	0.2908117109291848	1.002112745386426
7	0.1957016502097712	0.7867667028677909
8	0.13778393490050728	0.6330558322754498
9	0.10037746314358635	0.518838468597153
>10	0.7488984956290513	8.530309503640886
>50	0.0991795563898818	3.9626257975746504
>100	0.09670990575554612	11.461862447805334
>500	0.008150148018652308	3.117246801770345
>1k	0.00201464333045338	1.7504568052390275
>5k	2.7472409051636996E-4	1.0913598283061943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8323	0.43755957103074905	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6472	0.3402481729798159	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5956	0.3131208464567033	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4321	0.22716507346195683	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2370	0.12459644158871502	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2010	0.1056703998284039	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1966	0.10335721694658807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.257233822308651E-5	0.0
2	0.0	0.0	0.0	0.0014720254702464224	0.0
3	0.0	0.0	0.0	0.0021028935289234604	0.0
4	0.0	0.0	0.0	0.004048070043177661	0.0
5	1.0514467644617302E-4	0.0	0.0	0.0046789381018547	0.0
6	1.0514467644617302E-4	0.0	0.0	0.004731510440077786	0.0
7	1.0514467644617302E-4	0.0	0.0	0.0053098061605317375	0.0
8	1.0514467644617302E-4	0.0	0.0	0.005940674219208776	0.0
9	1.0514467644617302E-4	0.0	0.0	0.009147586850817053	0.0
10	1.5771701466925952E-4	0.0	0.0	0.013195656893994715	0.0
11	2.1028935289234605E-4	0.0	0.0	0.014299675996679532	0.0
12	2.1028935289234605E-4	0.0	0.0	0.015876846143372127	0.0
13	2.1028935289234605E-4	0.0	0.0	0.016980865246056943	0.0
14	2.6286169111543255E-4	0.0	0.0	0.017979739672295585	0.0
15	3.1543402933851904E-4	0.0	0.0	0.019241475789649663	0.0
16	3.1543402933851904E-4	0.0	0.0	0.021081507627457693	0.0
17	5.257233822308651E-4	0.0	0.0	0.02392041389150436	0.0
18	5.257233822308651E-4	0.0	0.0	0.025707873391089302	0.0
19	5.257233822308651E-4	0.0	0.0	0.028546779655135975	0.0
20	5.257233822308651E-4	0.0	0.0	0.03091253487517487	0.0
21	5.257233822308651E-4	0.0	0.0	0.03459259855079092	0.0
22	5.257233822308651E-4	0.0	0.0	0.039849832373099574	0.0
23	5.257233822308651E-4	0.0	0.0	0.0463688023127623	0.0
24	5.257233822308651E-4	0.0	0.0	0.056410118913371825	0.0
25	5.257233822308651E-4	0.0	0.0	0.06219307611791134	0.0
26	5.257233822308651E-4	0.0	0.0	0.07128809063050531	0.0
27	5.257233822308651E-4	0.0	0.0	0.09373647905176324	0.0
28	5.257233822308651E-4	0.0	0.0	0.13768695380626358	0.0
29	5.257233822308651E-4	0.0	0.0	0.20172006176198296	0.0
30	5.257233822308651E-4	0.0	0.0	0.2806837137730589	0.0
31	5.257233822308651E-4	0.0	0.0	0.5492232174165848	5.257233822308651E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	315	0.0	19.968254	36
GGTATCA	3395	0.0	19.5081	1
GCTTTAT	760	0.0	17.526316	1
AAGACGG	325	0.0	17.076923	5
CGAATTA	80	3.3840217E-4	16.1875	15
ACGTTCG	195	1.8189894E-12	16.128206	7
TATTGAG	780	0.0	15.653847	5
TGCGCTA	85	5.3664454E-4	15.235294	5
TATACTG	235	0.0	14.957447	5
ATGCCGT	425	0.0	14.8	34
GTATAGA	215	1.2732926E-11	14.627907	1
ATGCGTT	130	4.44736E-6	14.230768	36
TGCCGTC	445	0.0	14.134832	35
CGTTCGA	210	1.364242E-10	14.095238	8
CGGACCA	355	0.0	14.070422	9
CCGTCTT	530	0.0	13.962264	37
AGACGTT	240	5.456968E-12	13.875001	5
CTAGTAC	80	0.0063006203	13.875001	3
AATGCGT	135	6.5719105E-6	13.703704	35
CAGTCGG	405	0.0	13.703704	10
>>END_MODULE