FastQCFastQC Report
Fri 10 Feb 2017
ERR1630568.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630568.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163696
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT51393.139355879190695No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT43852.6787459681360573Illumina PCR Primer Index 10 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36952.2572329195582057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27421.6750562017398103No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC22401.3683901866875183No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC21791.3311259896393315No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG21021.284087576971948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19911.216278956113772No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.8485240934415014No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC10290.6286042420095788Illumina PCR Primer Index 10 (95% over 22bp)
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG5970.36470042029127164No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4840.2956700224806959No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.25474049457531034No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.24007917114651547No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3520.2150327436223243No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.19120809305053268No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2670.16310722314534257No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT2290.13989346104975076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2210.13500635324015248No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA2110.12889746847815464No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.12645391457335547No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.10812726028736194No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.10446192943016322No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1700.10385104095396346No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT1650.10079659857296452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGT200.001837327337.024
TCAGATC200.001837327337.020
ACTGTAT200.001837327337.03
TTATACA5750.031.8521752
TATACAC5950.030.7815133
TCTGAAC250.0054827529.613
TACGCTT250.0054827529.64
TCCTTGT250.0054827529.619
GTACGCT250.0054827529.63
TCCGGTC250.0054827529.68
CTTATAC6300.029.365081
CGTCTTC1450.029.34482837
GCCGTCT5550.028.036
CCGTCTT5600.027.75000237
AGATCAC400.00192415623.12500222
CCAACAC400.00192415623.1250023
TACACAT8000.022.8937515
CTGAACT502.6884052E-422.214
ATACACA8250.022.24
CTGTATC450.003812012520.5555574
Produced by FastQC (version 0.11.5)