##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630568.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	163696
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.864107858469357	33.0	31.0	34.0	30.0	34.0
2	31.992400547356073	33.0	31.0	34.0	30.0	34.0
3	32.02526634737562	33.0	31.0	34.0	30.0	34.0
4	35.67419484898837	37.0	35.0	37.0	33.0	37.0
5	35.70229571889356	37.0	35.0	37.0	35.0	37.0
6	35.760714983872546	37.0	35.0	37.0	35.0	37.0
7	35.71040220897273	37.0	35.0	37.0	33.0	37.0
8	35.756463200078194	37.0	35.0	37.0	35.0	37.0
9	37.37520159319715	39.0	37.0	39.0	34.0	39.0
10	37.31439986316098	39.0	37.0	39.0	34.0	39.0
11	37.45459265956407	39.0	37.0	39.0	35.0	39.0
12	37.40049237611181	39.0	37.0	39.0	34.0	39.0
13	37.47902819861206	39.0	37.0	39.0	35.0	39.0
14	38.586978301241324	40.0	38.0	41.0	34.0	41.0
15	38.54752712344834	40.0	38.0	41.0	34.0	41.0
16	38.42618023653602	40.0	38.0	41.0	34.0	41.0
17	38.45853289023556	40.0	38.0	41.0	34.0	41.0
18	38.5543507477275	40.0	38.0	41.0	34.0	41.0
19	38.590851334180435	40.0	38.0	41.0	34.0	41.0
20	38.650706187078484	40.0	38.0	41.0	34.0	41.0
21	38.57268961978301	40.0	38.0	41.0	34.0	41.0
22	38.47785529273776	40.0	38.0	41.0	34.0	41.0
23	38.441800654872445	40.0	38.0	41.0	34.0	41.0
24	38.35900693969309	40.0	38.0	41.0	34.0	41.0
25	38.27357418629655	40.0	37.0	41.0	34.0	41.0
26	37.94514221483726	40.0	37.0	41.0	33.0	41.0
27	37.69761631316587	40.0	36.0	41.0	33.0	41.0
28	37.599709217085326	40.0	36.0	41.0	33.0	41.0
29	37.412502443553905	39.0	36.0	41.0	32.0	41.0
30	37.24673174665233	39.0	35.0	41.0	32.0	41.0
31	37.02259676473463	39.0	35.0	41.0	32.0	41.0
32	36.73728130192552	39.0	35.0	41.0	31.0	41.0
33	36.496554588994236	38.0	35.0	41.0	31.0	41.0
34	36.286787704036755	38.0	35.0	41.0	30.0	41.0
35	36.10724147199687	38.0	35.0	40.0	30.0	41.0
36	35.88685734532304	38.0	35.0	40.0	30.0	41.0
37	35.48789219040172	38.0	35.0	40.0	27.0	41.0
38	35.20568614993647	38.0	35.0	40.0	26.0	41.0
39	34.886478594467796	38.0	34.0	40.0	24.0	41.0
40	34.44944897859447	37.0	34.0	40.0	22.0	41.0
41	34.154829684292835	37.0	33.0	40.0	21.0	41.0
42	33.787807887792006	37.0	33.0	40.0	18.0	41.0
43	32.76839385201838	35.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	1.0
14	1.0
15	0.0
16	3.0
17	6.0
18	5.0
19	24.0
20	44.0
21	65.0
22	151.0
23	282.0
24	416.0
25	699.0
26	1075.0
27	1624.0
28	2251.0
29	2935.0
30	3663.0
31	4552.0
32	5619.0
33	7109.0
34	8916.0
35	11186.0
36	15450.0
37	25130.0
38	32956.0
39	39530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.442063336917215	20.02675691525755	14.832372202130777	23.698807545694457
2	16.971092757306224	21.50388525070863	34.46754960414427	27.057472387840875
3	21.249755644609518	24.668287557423515	33.05151011631317	21.030446681653796
4	12.781008699051903	19.130583520672467	27.70257061870785	40.385837161567785
5	11.909881731991009	39.623448343270454	32.07958655067931	16.387083374059234
6	30.870027367803733	30.59940377284723	17.820838627700127	20.70973023164891
7	25.284674029909098	34.15660736975858	18.04931091779885	22.509407682533478
8	33.264099306030694	29.593270452546182	19.381658684390576	17.760971557032548
9	25.805151011631317	12.068101847326753	19.470848401915745	42.65589873912619
10	20.749437982601897	28.649447756817516	25.238857394194113	25.362256866386474
11	29.61465154921318	21.987708923858857	20.07746065878213	28.320178868145828
12	19.84898836868341	30.547478252370247	24.200957873130683	25.402575505815662
13	31.956187078486952	16.785993549017693	28.083765027856515	23.174054344638844
14	23.676204672075066	22.95291271625452	25.65059622715277	27.720286384517646
15	29.416112794448246	23.681091779884664	22.60409539634444	24.29870002932265
16	28.37027172319421	22.84112012510996	26.48384810868928	22.30476004300655
17	18.425618219137917	33.97944971166064	25.8540220897273	21.740909979474146
18	23.241252077020818	20.96752516860522	28.733139478056884	27.058083276317074
19	20.073184439448735	29.215741374254716	30.01905972045743	20.69201446583912
20	19.886863454207802	21.372544228325676	31.99772749486854	26.74286482259799
21	24.029298211318544	21.850259016713906	32.18832469944287	21.93211807252468
22	21.444018180041052	26.51133809011827	28.401427035480403	23.643216694360277
23	23.337161567784186	22.026194897859448	30.05754569445802	24.57909783989835
24	21.65049848499658	25.23152673247972	30.22798357931776	22.88999120320594
25	19.554540123155117	26.521723194213664	32.030715472583324	21.893021210047895
26	22.61142605805884	27.234019157462612	30.08198123350601	20.072573550972532
27	18.567955234092466	28.94328511386961	30.525486267227052	21.96327338481087
28	19.17456749095885	24.696388427328706	33.346569250317664	22.78247483139478
29	17.09815756035578	28.77651255986707	30.6788192747532	23.446510605023946
30	17.858102824748315	24.916919167236827	36.18170266836086	21.043275339653995
31	17.517227055028833	26.660394878311017	32.02888280715472	23.793495259505423
32	18.99985338676571	26.536995406118656	33.27020819079269	21.19294301632294
33	20.155043495259505	26.072720164206824	33.73937054051412	20.032865800019547
34	18.932655654383733	28.89074870491643	29.325090411494475	22.851505229205358
35	17.891090802463104	25.713517740201347	38.0583520672466	18.337039390088947
36	19.540489688202523	30.243866679698954	28.408757697194797	21.806885934903725
37	18.32543250904115	28.673883295865505	31.100332323331052	21.90035187176229
38	18.809256182191376	24.753811944091485	33.43270452546183	23.004227348255302
39	21.551534551852214	25.67136643534356	29.59815756035578	23.17894145244844
40	17.44086599550386	24.536946535040563	36.42361450493598	21.598572964519597
41	19.00351871762291	26.245601602971362	29.064241032157167	25.68663864724856
42	19.166015052292053	24.659124230280522	34.3911885446193	21.78367217280813
43	17.09388134102238	25.668922881438768	34.19203890137816	23.04515687616069
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	23.5
2	34.0
3	133.5
4	233.0
5	233.0
6	377.0
7	521.0
8	588.5
9	656.0
10	976.0
11	1296.0
12	1296.0
13	2383.5
14	3471.0
15	4975.0
16	6479.0
17	5856.5
18	5234.0
19	5234.0
20	6229.5
21	7225.0
22	5228.0
23	3231.0
24	2866.0
25	2501.0
26	2501.0
27	2710.5
28	2920.0
29	3067.5
30	3215.0
31	3484.0
32	3753.0
33	3753.0
34	3791.5
35	3830.0
36	3841.0
37	3852.0
38	4298.5
39	4745.0
40	4745.0
41	5164.5
42	5584.0
43	6060.5
44	6537.0
45	7141.0
46	7745.0
47	7745.0
48	9544.0
49	11343.0
50	14521.5
51	17700.0
52	12813.0
53	7926.0
54	7926.0
55	10833.0
56	13740.0
57	12307.0
58	10874.0
59	8413.0
60	5952.0
61	5952.0
62	5749.5
63	5547.0
64	5126.5
65	4706.0
66	4308.5
67	3911.0
68	3911.0
69	3483.0
70	3055.0
71	2658.5
72	2262.0
73	1877.0
74	1492.0
75	1492.0
76	1234.5
77	977.0
78	760.5
79	544.0
80	429.0
81	314.0
82	314.0
83	239.5
84	165.0
85	117.0
86	69.0
87	50.0
88	31.0
89	31.0
90	22.0
91	13.0
92	6.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	163696.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.700713517740205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.63460629549107	42.205673932166945
2	10.597646280366764	10.958117486071743
3	3.2056432555061916	4.97202130779005
4	1.410813876547878	2.917603362330173
5	0.7798468664335003	2.0159319714592905
6	0.48090556763399184	1.491789658879875
7	0.37101805463654414	1.3427328706871273
8	0.21386709518858116	0.8845665135372885
9	0.1725115795443804	0.8027074577265175
>10	1.0208904433311277	9.568957091193433
>50	0.05907930806314396	2.0721337112696707
>100	0.040173929482937895	3.9756622031082003
>500	0.0011815861612628792	0.36470042029127164
>1k	0.010634275451365914	13.288046134297723
>5k	0.0011815861612628792	3.139355879190695
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	5139	3.139355879190695	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	4385	2.6787459681360573	Illumina PCR Primer Index 10 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3695	2.2572329195582057	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2742	1.6750562017398103	No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	2240	1.3683901866875183	No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	2179	1.3311259896393315	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	2102	1.284087576971948	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1991	1.216278956113772	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1389	0.8485240934415014	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	1029	0.6286042420095788	Illumina PCR Primer Index 10 (95% over 22bp)
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	597	0.36470042029127164	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	484	0.2956700224806959	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	417	0.25474049457531034	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	393	0.24007917114651547	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	352	0.2150327436223243	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	313	0.19120809305053268	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	267	0.16310722314534257	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT	229	0.13989346104975076	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	221	0.13500635324015248	No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	211	0.12889746847815464	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	207	0.12645391457335547	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	177	0.10812726028736194	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	171	0.10446192943016322	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	170	0.10385104095396346	No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	165	0.10079659857296452	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.10888476199785E-4	0.0
2	0.0	0.0	0.0	0.0018326654285993548	0.0
3	0.0	0.0	0.0	0.0018326654285993548	0.0
4	0.0	0.0	0.0	0.0030544423809989248	0.0
5	0.0	0.0	0.0	0.0030544423809989248	0.0
6	0.0	0.0	0.0	0.0030544423809989248	0.0
7	0.0	0.0	0.0	0.0036653308571987096	0.0
8	0.0	0.0	0.0	0.0036653308571987096	0.0
9	0.0	0.0	0.0	0.004276219333398495	0.0
10	0.0	0.0	0.0	0.00488710780959828	0.0
11	0.0	0.0	0.0	0.005497996285798065	0.0
12	0.0	0.0	0.0	0.0061088847619978496	0.0
13	0.0	0.0	0.0	0.0061088847619978496	0.0
14	0.0	0.0	0.0	0.007941550190597205	0.0
15	0.0	0.0	0.0	0.00855243866679699	0.0
16	0.0	0.0	0.0	0.00977421561919656	0.0
17	0.0	0.0	0.0	0.014661323428794839	0.0
18	0.0	0.0	0.0	0.01832665428599355	0.0
19	0.0	0.0	0.0	0.022602873619392044	0.0
20	0.0	0.0	0.0	0.024435539047991398	0.0
21	0.0	0.0	0.0	0.029933535333789462	0.0
22	0.0	0.0	0.0	0.03909686247678624	0.0
23	0.0	0.0	0.0	0.04276219333398495	0.0
24	0.0	0.0	0.0	0.0690303978105757	0.0
25	0.0	0.0	0.0	0.07452839409637377	0.0
26	0.0	0.0	0.0	0.09529860228716645	0.0
27	0.0	0.0	0.0	0.12401036066855635	0.0
28	0.0	0.0	0.0	0.17043788485974	0.0
29	0.0	0.0	0.0	0.22541784771772067	0.0
30	0.0	0.0	0.0	0.32071645000488713	0.0
31	0.0	0.0	0.0	0.6286042420095788	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCACGT	20	0.0018373273	37.0	24
TCAGATC	20	0.0018373273	37.0	20
ACTGTAT	20	0.0018373273	37.0	3
TTATACA	575	0.0	31.852175	2
TATACAC	595	0.0	30.781513	3
TCTGAAC	25	0.00548275	29.6	13
TACGCTT	25	0.00548275	29.6	4
TCCTTGT	25	0.00548275	29.6	19
GTACGCT	25	0.00548275	29.6	3
TCCGGTC	25	0.00548275	29.6	8
CTTATAC	630	0.0	29.36508	1
CGTCTTC	145	0.0	29.344828	37
GCCGTCT	555	0.0	28.0	36
CCGTCTT	560	0.0	27.750002	37
AGATCAC	40	0.001924156	23.125002	22
CCAACAC	40	0.001924156	23.125002	3
TACACAT	800	0.0	22.893751	5
CTGAACT	50	2.6884052E-4	22.2	14
ATACACA	825	0.0	22.2	4
CTGTATC	45	0.0038120125	20.555557	4
>>END_MODULE