FastQCFastQC Report
Fri 10 Feb 2017
ERR1630567.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630567.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982698
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35320.17814109864437247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27680.1396077466159748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24200.12205590563968895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC1501.2732926E-1118.53
TATACTG2800.017.8392855
GGTATCA18350.017.6430511
ATAAGAC1557.21775E-915.5161293
GCGCATT750.004105800814.811
TAGACAG2600.014.2307695
GTGTAGG3950.014.0506321
CTTATAC9350.014.04812937
CCTAGAC1601.7851926E-713.8753
GTACGAC1356.572247E-613.7037043
TCTTATA14850.013.57912437
GTATTAG2051.4260877E-913.5365851
ACCGTTA1102.457769E-413.45454518
CTAGACT1805.164111E-813.3611114
GTATAGG1805.164111E-813.3611111
GTATAAT2850.012.9824561
TATATAC3000.012.953
CCGTTAT1000.001833295612.94999919
TGGACCG2152.903107E-912.9069775
CGAATTA1153.5792045E-412.86956515
Produced by FastQC (version 0.11.5)