FastQCFastQC Report
Fri 10 Feb 2017
ERR1630566.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630566.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653311
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80830.48889773309437845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79550.48115569303053085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66440.4018602670640914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44650.27006413191468515No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC28590.17292572298859682No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT24200.1463729449571194No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA22400.13548570111733366No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA20970.12683639073350386No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG19450.11764271815768478No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG17090.10336833178996573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAC400.001931543223.12500215
GTTCTAG1450.021.6896551
TCTAACG450.003826506720.5555572
AAGACGG3550.018.2394375
GACGGAC3400.017.4117647
AGTCGGT2650.016.75471511
GCTTTAT7100.016.6760581
GTCCTAA2250.016.4444431
ACGGACC3650.016.2191778
TCTAGCG1951.8189894E-1216.12820628
GAACCGT1059.347976E-615.8571426
TTAACGG1302.5907684E-715.65384635
CAAGACG4150.015.6024094
GTAATAC1201.9366507E-615.4166673
CTAGCGG2055.456968E-1215.34146429
CTAGACT1353.9749102E-715.0740754
TTTCGGA2850.014.92982430
GTCGGTG3100.014.91935412
TAACGGC1252.9604616E-614.79999936
GAGCGAA4050.014.61728416
Produced by FastQC (version 0.11.5)