##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630566.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1653311
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.997780816797324	33.0	31.0	34.0	30.0	34.0
2	32.14657012503999	33.0	31.0	34.0	30.0	34.0
3	32.25378467813981	34.0	31.0	34.0	30.0	34.0
4	35.83794035121039	37.0	35.0	37.0	35.0	37.0
5	35.818458838052855	37.0	35.0	37.0	35.0	37.0
6	35.85803215486983	37.0	35.0	37.0	35.0	37.0
7	35.83296367108185	37.0	35.0	37.0	35.0	37.0
8	35.82880655847569	37.0	35.0	37.0	35.0	37.0
9	37.54602733544989	39.0	37.0	39.0	35.0	39.0
10	37.460703400630614	39.0	37.0	39.0	35.0	39.0
11	37.56265034225261	39.0	37.0	39.0	35.0	39.0
12	37.496757718299826	39.0	37.0	39.0	35.0	39.0
13	37.54707190601163	39.0	37.0	39.0	35.0	39.0
14	38.81030126818246	40.0	38.0	41.0	35.0	41.0
15	38.781328497784145	40.0	38.0	41.0	35.0	41.0
16	38.75711345294382	40.0	38.0	41.0	35.0	41.0
17	38.765999258457725	40.0	38.0	41.0	35.0	41.0
18	38.75670457645295	40.0	38.0	41.0	35.0	41.0
19	38.77486389433083	40.0	38.0	41.0	35.0	41.0
20	38.765069003956306	40.0	38.0	41.0	35.0	41.0
21	38.69519830207384	40.0	38.0	41.0	34.0	41.0
22	38.63658682486235	40.0	38.0	41.0	34.0	41.0
23	38.61801984018736	40.0	38.0	41.0	34.0	41.0
24	38.55915190789876	40.0	38.0	41.0	34.0	41.0
25	38.51088996565075	40.0	38.0	41.0	34.0	41.0
26	38.316746214112165	40.0	38.0	41.0	34.0	41.0
27	38.192843330746605	40.0	38.0	41.0	34.0	41.0
28	38.09101614880685	40.0	37.0	41.0	33.0	41.0
29	37.985601015175	40.0	37.0	41.0	33.0	41.0
30	37.89984280029589	40.0	37.0	41.0	33.0	41.0
31	37.83082795674861	40.0	37.0	41.0	33.0	41.0
32	37.730531037415226	40.0	37.0	41.0	33.0	41.0
33	37.674445400774566	40.0	37.0	41.0	33.0	41.0
34	37.59105637112437	40.0	37.0	41.0	33.0	41.0
35	37.509839951467086	40.0	37.0	41.0	32.0	41.0
36	37.454519446129616	40.0	36.0	41.0	32.0	41.0
37	37.251200167421615	40.0	36.0	41.0	31.0	41.0
38	37.127315429462456	40.0	36.0	41.0	31.0	41.0
39	37.046040944504696	39.0	36.0	41.0	31.0	41.0
40	36.90884292187011	39.0	35.0	41.0	31.0	41.0
41	36.76852933295672	39.0	35.0	41.0	30.0	41.0
42	36.64619481755096	39.0	35.0	41.0	30.0	41.0
43	35.792789136466155	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	6.0
12	5.0
13	1.0
14	5.0
15	6.0
16	11.0
17	21.0
18	42.0
19	99.0
20	203.0
21	426.0
22	843.0
23	1479.0
24	2555.0
25	3971.0
26	6238.0
27	9086.0
28	13479.0
29	18462.0
30	24834.0
31	32607.0
32	41913.0
33	54704.0
34	71556.0
35	95055.0
36	133642.0
37	207455.0
38	365014.0
39	569585.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5882837530265	18.977373283066527	11.928608713061244	24.505734250845727
2	19.019047233097705	20.968408242611343	33.61176451375452	26.400780010536433
3	21.252262883389754	23.56398765870426	28.423871854720616	26.759877603185366
4	14.514147670946361	14.933125104714115	33.89991356738085	36.65281365695867
5	14.476344740947106	37.221430208835486	33.66680557983344	14.635419470383976
6	35.51043935472515	33.88158670691721	15.38512717812922	15.222846760228414
7	29.389026021117626	30.139096636990864	21.296053797500893	19.175823544390617
8	28.859059184872056	31.48415512870839	19.84000590330555	19.816779783114004
9	27.08032548020306	13.597925617140394	20.021701906053973	39.300046996602575
10	17.986997001774014	26.67876763657896	31.64183871032129	23.692396651325733
11	35.5741902158759	22.051568035294025	21.07824843601718	21.295993312812893
12	23.231624298150802	25.60504345522409	27.8812032339953	23.282129012629806
13	30.65146242902878	19.442621503153372	25.244736168815184	24.66117989900267
14	23.221402385879003	20.722417016520183	25.411794877067894	30.644385720532917
15	26.081602311966712	27.592812241616972	22.537562503364462	23.78802294305185
16	25.179231251712476	26.045251014479433	24.45033027663882	24.32518745716928
17	24.018530089015318	26.19670467322845	25.368185417020754	24.416579820735482
18	23.520015290529127	24.46031025015862	27.67730935075131	24.342365108560944
19	24.6189615867795	24.936022321269256	27.53631954302608	22.908696548925157
20	25.131569317569415	24.219339253171363	26.683969319746858	23.965122109512365
21	25.398609215084157	24.480875044078214	26.691408936370713	23.429106804466915
22	25.10713350361789	24.714285455065625	26.74118783459373	23.43739320672275
23	24.232524915155103	24.692450482698053	26.535479410709783	24.539545191437064
24	23.84191480005879	25.493388721178288	26.961170644845406	23.703525833917517
25	24.153592397316658	25.322821901021648	26.458663856951294	24.0649218447104
26	24.13974140376493	25.68905668685444	26.662255316755285	23.508946592625342
27	24.380409977312194	24.755052134776818	26.565903208773182	24.298634679137805
28	23.58068143259193	25.412883601451874	26.888891442686823	24.117543523269365
29	23.706066190813466	25.922890490657835	27.170387180633288	23.20065613789541
30	23.812216818251375	25.767626296564895	26.569713744117106	23.850443141066624
31	23.191220526567598	26.314347391386132	26.791994972512732	23.702437109533538
32	23.01738753325902	25.66776607667886	26.576004151668986	24.73884223839314
33	22.609055404579053	25.992750305296465	27.108934737626495	24.289259552497988
34	24.527327284461304	24.917332552677625	26.550056220517494	24.005283942343574
35	23.522071769921084	25.219453569231682	26.98881214726086	24.269662513586372
36	22.646555911138318	26.061400426175112	27.164762104649398	24.127281558037176
37	23.481849452401875	24.817835240919585	27.619969866528436	24.0803454401501
38	23.338500741844697	24.885275668038258	27.421096212388353	24.355127377728692
39	23.29325819522159	24.581279626156242	27.667994708799494	24.457467469822678
40	22.5407077071404	24.637953778811124	28.216106951444708	24.60523156260377
41	21.519907627784487	25.0561449116349	28.76222319938596	24.661724261194657
42	22.414839071414878	24.855880109670835	28.906055787447126	23.82322503146716
43	22.36995943291976	24.43230583961517	28.561172096477915	24.636562630987154
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	426.0
1	394.5
2	363.0
3	946.5
4	1530.0
5	1530.0
6	2190.0
7	2850.0
8	2654.0
9	2458.0
10	3652.5
11	4847.0
12	4847.0
13	8341.5
14	11836.0
15	16224.0
16	20612.0
17	19520.5
18	18429.0
19	18429.0
20	20907.0
21	23385.0
22	21196.0
23	19007.0
24	21291.5
25	23576.0
26	23576.0
27	26790.0
28	30004.0
29	33669.5
30	37335.0
31	41717.0
32	46099.0
33	46099.0
34	51270.0
35	56441.0
36	62158.5
37	67876.0
38	72877.0
39	77878.0
40	77878.0
41	81310.5
42	84743.0
43	86439.5
44	88136.0
45	91980.0
46	95824.0
47	95824.0
48	102247.5
49	108671.0
50	108611.0
51	108551.0
52	107858.0
53	107165.0
54	107165.0
55	104432.5
56	101700.0
57	100134.5
58	98569.0
59	99858.0
60	101147.0
61	101147.0
62	93488.5
63	85830.0
64	79207.0
65	72584.0
66	63268.5
67	53953.0
68	53953.0
69	47950.0
70	41947.0
71	35411.5
72	28876.0
73	21406.5
74	13937.0
75	13937.0
76	10175.5
77	6414.0
78	5100.5
79	3787.0
80	3195.5
81	2604.0
82	2604.0
83	2030.0
84	1456.0
85	1255.5
86	1055.0
87	950.0
88	845.0
89	845.0
90	664.5
91	484.0
92	270.5
93	57.0
94	38.5
95	20.0
96	20.0
97	11.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1653311.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.575665633776836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.82028123600793	49.11915201438854
2	8.307078192607614	9.399569564372152
3	1.962656006721706	3.331157099712327
4	0.8128645875530869	1.8395342044376546
5	0.44298813935773984	1.2531174426016212
6	0.27107597069797407	0.9201782087736042
7	0.1951883337834882	0.773003693542406
8	0.13811475836106007	0.6251147510500176
9	0.11286208821317477	0.5746722988930563
>10	0.7246500057178649	8.230781955877234
>50	0.09489690516361896	3.793591600043092
>100	0.1031725218615063	11.958651978855814
>500	0.011165230387455682	4.3395832050328815
>1k	0.0026839496123691542	2.4642214538243596
>5k	3.2207395348429855E-4	1.3776705285954036
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8083	0.48889773309437845	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7955	0.48115569303053085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6644	0.4018602670640914	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4465	0.27006413191468515	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2859	0.17292572298859682	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2420	0.1463729449571194	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2240	0.13548570111733366	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2097	0.12683639073350386	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1945	0.11764271815768478	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	1709	0.10336833178996573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.048468799880966E-5	0.0
2	0.0	0.0	0.0	0.0010887243839785739	0.0
3	0.0	0.0	0.0	0.0021774487679571478	0.0
4	0.0	0.0	0.0	0.0036895659679273894	0.0
5	1.8145406399642898E-4	0.0	0.0	0.004233928159916676	0.0
6	2.4193875199523865E-4	0.0	0.0	0.004233928159916676	0.0
7	2.4193875199523865E-4	0.0	0.0	0.0048992597279035825	0.0
8	2.4193875199523865E-4	0.0	0.0	0.005927499423883347	0.0
9	2.4193875199523865E-4	0.0	0.0	0.010161427583800023	0.0
10	4.838775039904773E-4	0.0	0.0	0.016330865759678608	0.0
11	5.443621919892869E-4	0.0	0.0	0.01826637577564052	0.0
12	5.443621919892869E-4	0.0	0.0	0.02014140110360362	0.0
13	6.653315679869062E-4	0.0	0.0	0.02153254892757624	0.0
14	6.653315679869062E-4	0.0	0.0	0.023649513007534578	0.0
15	7.258162559857159E-4	0.0	0.0	0.02522211489550363	0.0
16	7.258162559857159E-4	0.0	0.0	0.02891168086343102	0.0
17	7.863009439845256E-4	0.0	0.0	0.03338754777534293	0.0
18	7.863009439845256E-4	0.0	0.0	0.036169843423288175	0.0
19	9.072703199821449E-4	0.0	0.0	0.04058522564720128	0.0
20	9.072703199821449E-4	0.0	0.0	0.04433527630312748	0.0
21	9.072703199821449E-4	0.0	0.0	0.05189586230297869	0.0
22	0.0010887243839785739	0.0	0.0	0.06326698364675491	0.0
23	0.0010887243839785739	0.0	0.0	0.0795373647184347	0.0
24	0.0010887243839785739	0.0	0.0	0.10469899492593952	0.0
25	0.0010887243839785739	0.0	0.0	0.11183618810979906	0.0
26	0.0010887243839785739	0.0	0.0	0.12375167164556457	0.0
27	0.0011492090719773835	0.0	0.0	0.14304628711718484	0.0
28	0.0012096937599761933	0.0	0.0	0.18508314527635755	0.0
29	0.0012096937599761933	0.0	0.0	0.2522816336430351	0.0
30	0.0012701784479750029	0.0	0.0	0.3410126709372889	0.0
31	0.0012701784479750029	0.0	0.0	0.6462788912672812	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAAC	40	0.0019315432	23.125002	15
GTTCTAG	145	0.0	21.689655	1
TCTAACG	45	0.0038265067	20.555557	2
AAGACGG	355	0.0	18.239437	5
GACGGAC	340	0.0	17.411764	7
AGTCGGT	265	0.0	16.754715	11
GCTTTAT	710	0.0	16.676058	1
GTCCTAA	225	0.0	16.444443	1
ACGGACC	365	0.0	16.219177	8
TCTAGCG	195	1.8189894E-12	16.128206	28
GAACCGT	105	9.347976E-6	15.857142	6
TTAACGG	130	2.5907684E-7	15.653846	35
CAAGACG	415	0.0	15.602409	4
GTAATAC	120	1.9366507E-6	15.416667	3
CTAGCGG	205	5.456968E-12	15.341464	29
CTAGACT	135	3.9749102E-7	15.074075	4
TTTCGGA	285	0.0	14.929824	30
GTCGGTG	310	0.0	14.919354	12
TAACGGC	125	2.9604616E-6	14.799999	36
GAGCGAA	405	0.0	14.617284	16
>>END_MODULE