FastQCFastQC Report
Fri 10 Feb 2017
ERR1630565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211509
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56092.651896609600537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43662.06421476154679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33381.5781834342746646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22481.0628389335678385No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT13520.6392162981244297No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT12570.5943009517325504RNA PCR Primer, Index 33 (95% over 22bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC7110.336155908259223No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC6880.32528166650118906No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.3110978729037535No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6400.3025875967452922No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG6280.2969140793063179No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.2605090090729No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5450.25767225035341285No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5300.25058035355469505No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4110.1943179722848673No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.17446066124845752No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3660.17304228188871396No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3490.16500479885016714No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.14041955661461214No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2960.13994676349469762No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2800.13238207357606532No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2750.13001810797649271No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC2640.12481738365743301RNA PCR Primer, Index 33 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2440.11536152125914265No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2230.10543286574093774No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10401448638119419No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA2160.10212331390153612No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.10023214142187802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC651.8189894E-1234.15384737
CGGTTCA250.005486334729.59999833
TAACGGT250.005486334729.59999830
ATAACGG250.005486334729.59999829
GGTTCAT250.005486334729.59999834
CTGTTTA358.8453875E-426.4285722
GTCGTAG358.8453875E-426.428571
CCGTCTT2550.026.11764737
GCCGTCT2650.024.43396236
TATACAC3200.021.968753
TGAACAA603.7078134E-521.58333219
TAATCGT603.7078134E-521.58333212
AAAAGAC450.003815648420.55555537
GGTTATG450.003815648420.5555558
TGCCGTC3150.020.55555535
TTATACA3500.020.0857142
ACAAACG656.868156E-519.92307722
CGAACCT656.868156E-519.92307727
GAACCTT656.868156E-519.92307728
ACCTTTA656.868156E-519.92307730
Produced by FastQC (version 0.11.5)