FastQCFastQC Report
Fri 10 Feb 2017
ERR1630564.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630564.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457413
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59860.4107277758603773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51060.35034681315454164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44860.3078056803390666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33680.2310944118105163No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC33010.2264972248772311No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT20910.14347340115670712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1705.456968E-1217.41176416
GTTATAC852.7239263E-517.4117643
ACGAACG1705.456968E-1217.41176415
ATAACGA1801.0913936E-1116.44444512
CAGGACA12950.016.4285724
GGTATCA30100.016.1029911
GACAGGC11600.015.7887937
GTCCTAC1302.59015E-715.6538461
CCGATAA1902.7284841E-1115.5789489
CGTACAC957.06175E-515.5789483
CCAGGAC14150.015.5583043
TGCATCA11000.015.47272814
GCATCAG11200.015.36160815
GCTGCAT11050.015.06787412
ACAGGCT12450.015.0080328
ATCAAGC11150.014.93273630
CGATAAC2006.184564E-1114.79999910
TATGACC1405.997499E-714.5357154
TATACTG2301.8189894E-1214.4782615
TAACGAA2059.276846E-1114.43902413
Produced by FastQC (version 0.11.5)