##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630564.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1457413
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.997197088265303	33.0	31.0	34.0	30.0	34.0
2	32.158787522822976	33.0	31.0	34.0	30.0	34.0
3	32.25583688357384	34.0	31.0	34.0	30.0	34.0
4	35.84151781272707	37.0	35.0	37.0	35.0	37.0
5	35.820186865356625	37.0	35.0	37.0	35.0	37.0
6	35.852262193352196	37.0	35.0	37.0	35.0	37.0
7	35.824931573960164	37.0	35.0	37.0	35.0	37.0
8	35.81472032978984	37.0	35.0	37.0	35.0	37.0
9	37.542862592827156	39.0	37.0	39.0	35.0	39.0
10	37.4467635460916	39.0	37.0	39.0	34.0	39.0
11	37.56542037157621	39.0	37.0	39.0	35.0	39.0
12	37.49103925929026	39.0	37.0	39.0	35.0	39.0
13	37.53578223880259	39.0	37.0	39.0	35.0	39.0
14	38.80805715332579	40.0	38.0	41.0	35.0	41.0
15	38.77766151392913	40.0	38.0	41.0	35.0	41.0
16	38.7589591968783	40.0	38.0	41.0	35.0	41.0
17	38.749342156272796	40.0	38.0	41.0	35.0	41.0
18	38.709956614906	40.0	38.0	41.0	35.0	41.0
19	38.74100203579905	40.0	38.0	41.0	34.0	41.0
20	38.72253506727331	40.0	38.0	41.0	34.0	41.0
21	38.65719600415256	40.0	38.0	41.0	34.0	41.0
22	38.59215541510883	40.0	38.0	41.0	34.0	41.0
23	38.55872631848351	40.0	38.0	41.0	34.0	41.0
24	38.50229550580378	40.0	38.0	41.0	34.0	41.0
25	38.45628864295845	40.0	38.0	41.0	34.0	41.0
26	38.25230391110824	40.0	38.0	41.0	34.0	41.0
27	38.135568298073366	40.0	38.0	41.0	33.0	41.0
28	38.03016852463921	40.0	37.0	41.0	33.0	41.0
29	37.91525188810584	40.0	37.0	41.0	33.0	41.0
30	37.83944427557597	40.0	37.0	41.0	33.0	41.0
31	37.78781169098944	40.0	37.0	41.0	33.0	41.0
32	37.681529532123015	40.0	37.0	41.0	33.0	41.0
33	37.62818912689814	40.0	37.0	41.0	33.0	41.0
34	37.532896989391475	40.0	36.0	41.0	32.0	41.0
35	37.46458553615207	40.0	36.0	41.0	32.0	41.0
36	37.40827548539776	40.0	36.0	41.0	32.0	41.0
37	37.20610492701794	40.0	36.0	41.0	31.0	41.0
38	37.09069426442608	39.0	36.0	41.0	31.0	41.0
39	37.01439674272152	39.0	36.0	41.0	31.0	41.0
40	36.88130886715022	39.0	35.0	41.0	31.0	41.0
41	36.732222094903776	39.0	35.0	41.0	30.0	41.0
42	36.62895212269961	39.0	35.0	41.0	30.0	41.0
43	35.75624273970384	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	1.0
12	2.0
13	2.0
14	4.0
15	1.0
16	9.0
17	23.0
18	49.0
19	102.0
20	226.0
21	437.0
22	742.0
23	1415.0
24	2328.0
25	3695.0
26	5740.0
27	8365.0
28	11900.0
29	16586.0
30	21963.0
31	28833.0
32	37515.0
33	48459.0
34	64421.0
35	86620.0
36	119170.0
37	180373.0
38	321007.0
39	497417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01051726586767	18.433072848945358	11.798645956911322	25.75776392827565
2	19.35305915344518	20.476076445043375	33.917976579047945	26.2528878224635
3	21.54001645381234	22.832649358829652	28.153584467820718	27.473749719537288
4	15.256691136966666	15.025047807313369	32.58781141653052	37.130449639189436
5	15.268492870586442	36.65831167966802	32.66184671057552	15.411348739170021
6	36.391125919694694	32.50718910837217	15.41169181282176	15.689993159111385
7	29.770421973730166	29.717382787171516	20.95377219772295	19.55842304137537
8	28.372190998707985	32.20912671974245	19.577429321681638	19.84125295986793
9	27.52253479281439	13.596900809859664	19.438004189615434	39.442560207710514
10	18.373378033543	26.158130879853548	31.503630062309036	23.964861024294418
11	36.04366092521475	21.367313177527578	20.6972217209535	21.891804176304177
12	22.8509008771021	24.746382802952905	28.201477549603304	24.201238770341693
13	30.393992643128616	19.414881025488313	24.935759458712113	25.255366872670958
14	23.364344904292743	20.481977311853264	25.09178935552242	31.061888428331567
15	26.408780489813115	26.60055866113449	22.312549702795295	24.678111146257102
16	25.5849234225302	25.34875152067396	23.73287462098938	25.33345043580646
17	24.065450218983912	25.961824136329238	25.341684203448168	24.631041441238686
18	24.078761476671335	23.999511463119926	26.757206090517926	25.16452096969082
19	24.67474902447007	24.692108551247998	26.297830470841145	24.33531195344079
20	24.70342998175534	24.413052442924553	26.48864803593765	24.394869539382455
21	26.32987354991344	24.120067544340554	25.668907852475588	23.88115105327042
22	25.614153297658248	24.510691204209103	25.765997695917353	24.109157802215293
23	24.812733247198977	24.703155522833953	25.745962194655874	24.7381490353112
24	24.938298203734973	24.898707504324445	25.92436049355948	24.238633798381105
25	24.815546451143224	24.554536016901178	26.04546549262289	24.584452039332707
26	25.09041706091547	25.052816188685018	26.039770470004044	23.81699628039547
27	25.446253052497813	24.598723903244995	25.511437046327977	24.44358599792921
28	24.305533160469956	24.451408077188827	26.548548695531053	24.694510066810164
29	23.993610596310035	24.778357267294858	26.8179301268755	24.41010200951961
30	23.745088042991245	25.243153450669098	26.64008074581467	24.371677760524985
31	24.0684692671192	25.22826405418368	26.06852004201966	24.634746636677455
32	23.83785515842112	25.066882208406266	26.223314873683712	24.8719477594889
33	24.034779434518562	25.159786553296836	26.18859582012786	24.616838192056747
34	24.67872867883023	25.0673625115187	26.112159010520696	24.141749799130377
35	24.564553767531923	24.830092773976904	26.59747099826885	24.007882460222326
36	24.11519589848588	25.286243501327355	25.781024321863466	24.817536278323303
37	23.952922061213947	24.68010097343718	26.47382725418258	24.89314971116629
38	24.061676408814797	24.33277320841793	26.96565764131375	24.63989274145352
39	23.505965707730066	24.486881892778506	27.034203756930946	24.972948642560482
40	23.60216355967732	24.039102162530458	27.773184402773953	24.58554987501827
41	22.622551054505482	24.372844210940894	28.141782734200945	24.86282200035268
42	22.96054721619747	24.59302888062615	27.74004348801609	24.706380415160286
43	22.657681796443423	23.525863979530854	28.208682096289795	25.607772127735927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	621.0
1	489.5
2	358.0
3	723.0
4	1088.0
5	1088.0
6	1509.0
7	1930.0
8	1924.0
9	1918.0
10	2774.0
11	3630.0
12	3630.0
13	6323.0
14	9016.0
15	11745.5
16	14475.0
17	14082.5
18	13690.0
19	13690.0
20	15574.0
21	17458.0
22	15995.5
23	14533.0
24	16075.0
25	17617.0
26	17617.0
27	20511.0
28	23405.0
29	26843.5
30	30282.0
31	34111.0
32	37940.0
33	37940.0
34	42689.0
35	47438.0
36	52382.0
37	57326.0
38	61780.0
39	66234.0
40	66234.0
41	70208.5
42	74183.0
43	76365.0
44	78547.0
45	81270.5
46	83994.0
47	83994.0
48	86723.0
49	89452.0
50	92300.0
51	95148.0
52	99354.5
53	103561.0
54	103561.0
55	102992.5
56	102424.0
57	99286.0
58	96148.0
59	89752.5
60	83357.0
61	83357.0
62	79430.5
63	75504.0
64	66858.5
65	58213.0
66	50835.0
67	43457.0
68	43457.0
69	37540.0
70	31623.0
71	28376.5
72	25130.0
73	23835.0
74	22540.0
75	22540.0
76	20210.5
77	17881.0
78	14041.0
79	10201.0
80	6873.5
81	3546.0
82	3546.0
83	2524.5
84	1503.0
85	1210.0
86	917.0
87	798.0
88	679.0
89	679.0
90	523.5
91	368.0
92	211.0
93	54.0
94	36.0
95	18.0
96	18.0
97	11.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1457413.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.9817529925204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.41551946570219	52.69769862777789
2	8.345835496592334	10.178873575388051
3	2.1653862855742285	3.961471548008366
4	0.8822524766913117	2.1520521044251577
5	0.4841158664360722	1.4761117093382274
6	0.3133682162849587	1.1465845896717635
7	0.2077594053079688	0.8868672915454441
8	0.15485794820334367	0.7554807317011859
9	0.11982797714249753	0.6576588093327449
>10	0.7551645680514799	8.793528414993792
>50	0.07389688957799763	3.141027094248298
>100	0.07414018583475557	9.184514749529853
>500	0.005737659266342232	2.282053901423806
>1k	0.001912553088780744	1.9250949245092746
>5k	2.2500624573891108E-4	0.7609819281060896
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5986	0.4107277758603773	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5106	0.35034681315454164	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4486	0.3078056803390666	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3368	0.2310944118105163	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3301	0.2264972248772311	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2091	0.14347340115670712	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.233767641704857E-4	0.0
3	0.0	0.0	0.0	0.0017839829890360522	0.0
4	0.0	0.0	0.0	0.0028818186745966996	0.0
5	2.7445892139016186E-4	0.0	0.0	0.0031562775959868617	0.0
6	2.7445892139016186E-4	0.0	0.0	0.0032248923263344022	0.0
7	2.7445892139016186E-4	0.0	0.0	0.003636580708419645	0.0
8	2.7445892139016186E-4	0.0	0.0	0.0039796543601573475	0.0
9	2.7445892139016186E-4	0.0	0.0	0.006792858304406507	0.0
10	4.1168838208524285E-4	0.0	0.0	0.009468832787960586	0.0
11	4.803031124327833E-4	0.0	0.0	0.010978356855606476	0.0
12	5.489178427803237E-4	0.0	0.0	0.011870348350124502	0.0
13	5.489178427803237E-4	0.0	0.0	0.012350651462557285	0.0
14	5.489178427803237E-4	0.0	0.0	0.013174028226727771	0.0
15	8.233767641704857E-4	0.0	0.0	0.01461493756402612	0.0
16	8.233767641704857E-4	0.0	0.0	0.01742814150827528	0.0
17	0.0012350651462557285	0.0	0.0	0.020858878025652304	0.0
18	0.0012350651462557285	6.861473034754047E-5	0.0	0.022985934666426056	0.0
19	0.0013722946069508095	6.861473034754047E-5	0.0	0.025456064958937513	0.0
20	0.0014409093372983498	6.861473034754047E-5	0.0	0.027171433217626027	0.0
21	0.0015095240676458903	6.861473034754047E-5	0.0	0.03128831703847845	0.0
22	0.0015781387979934309	6.861473034754047E-5	0.0	0.03705195438767185	0.0
23	0.0015781387979934309	6.861473034754047E-5	0.0	0.046863860827370145	0.0
24	0.0017153682586885117	6.861473034754047E-5	0.0	0.06250801934660936	0.0
25	0.0017839829890360522	6.861473034754047E-5	0.0	0.06813442723510768	0.0
26	0.0017839829890360522	6.861473034754047E-5	0.0	0.08096538181009776	0.0
27	0.0017839829890360522	6.861473034754047E-5	0.0	0.1073134382635533	0.0
28	0.0017839829890360522	2.0584419104262142E-4	0.0	0.15795110926003816	0.0
29	0.0017839829890360522	2.0584419104262142E-4	0.0	0.22409570931506717	0.0
30	0.0018525977193835927	2.0584419104262142E-4	0.0	0.3174803573180698	0.0
31	0.0018525977193835927	2.0584419104262142E-4	0.0	0.5733446867840482	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	170	5.456968E-12	17.411764	16
GTTATAC	85	2.7239263E-5	17.411764	3
ACGAACG	170	5.456968E-12	17.411764	15
ATAACGA	180	1.0913936E-11	16.444445	12
CAGGACA	1295	0.0	16.428572	4
GGTATCA	3010	0.0	16.102991	1
GACAGGC	1160	0.0	15.788793	7
GTCCTAC	130	2.59015E-7	15.653846	1
CCGATAA	190	2.7284841E-11	15.578948	9
CGTACAC	95	7.06175E-5	15.578948	3
CCAGGAC	1415	0.0	15.558304	3
TGCATCA	1100	0.0	15.472728	14
GCATCAG	1120	0.0	15.361608	15
GCTGCAT	1105	0.0	15.067874	12
ACAGGCT	1245	0.0	15.008032	8
ATCAAGC	1115	0.0	14.932736	30
CGATAAC	200	6.184564E-11	14.799999	10
TATGACC	140	5.997499E-7	14.535715	4
TATACTG	230	1.8189894E-12	14.478261	5
TAACGAA	205	9.276846E-11	14.439024	13
>>END_MODULE