FastQCFastQC Report
Fri 10 Feb 2017
ERR1630563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773778
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC86920.4900275006229641No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT56350.3176834981604237No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG35100.19788271136523286No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG31700.1787145854780023No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT31660.17848907811462314No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG30880.17409168452872908No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT30740.17330240875690195No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG30000.16913052253438704No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT28080.15830616909218628No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC28050.1581370385696519No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG27380.1543597902330506No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG25420.14330992942747064No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT25010.14099847895283402No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT24170.13626282432187117No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT23770.13400775068807935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23300.13135803916837394No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG22430.12645325401487673No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG21740.12256325199658583No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG21440.12087194677124194No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC21360.12042093204448358No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG20370.11483962480084882No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT19760.11140063750931628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19300.10880730283045567No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA18350.10345150295020009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA400.001931598323.1250026
TAATACG652.6842117E-622.769234
GGTATCA13750.019.509091
GACAGGC21300.018.6737087
CAGGACA24250.018.5381434
TATACCG609.239511E-418.55
AACACCG801.617477E-518.55
TGCATCA20900.018.41148414
GCATCAG21400.018.15420515
ACAGGCT21850.018.0343258
CCAGGAC23650.017.9133193
TCCAGGA24900.017.5341362
GCTGCAT22150.017.45598212
CTGCATC22800.016.87719313
ATCAAGC23250.016.55053730
TATTAGA3250.016.5076922
TCTAATA2600.016.3653852
ATTAGAG4400.015.9772733
GGACAGG26050.015.9078696
ATCAGAA24850.015.85714317
Produced by FastQC (version 0.11.5)