##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630563.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1773778
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98984765850067	33.0	31.0	34.0	30.0	34.0
2	32.160889355939695	33.0	31.0	34.0	30.0	34.0
3	32.255317745512684	34.0	31.0	34.0	30.0	34.0
4	35.85030031943118	37.0	35.0	37.0	35.0	37.0
5	35.827175103085054	37.0	35.0	37.0	35.0	37.0
6	35.8549266029909	37.0	35.0	37.0	35.0	37.0
7	35.82902990114885	37.0	35.0	37.0	35.0	37.0
8	35.812658630335925	37.0	35.0	37.0	35.0	37.0
9	37.56164694792697	39.0	37.0	39.0	35.0	39.0
10	37.451450519738096	39.0	37.0	39.0	35.0	39.0
11	37.56439362761292	39.0	37.0	39.0	35.0	39.0
12	37.47956677780421	39.0	37.0	39.0	35.0	39.0
13	37.51898490115448	39.0	37.0	39.0	35.0	39.0
14	38.79634317259544	40.0	38.0	41.0	35.0	41.0
15	38.781394853245445	40.0	38.0	41.0	35.0	41.0
16	38.771762869987114	40.0	38.0	41.0	35.0	41.0
17	38.73624320518126	40.0	38.0	41.0	35.0	41.0
18	38.658505743108776	40.0	38.0	41.0	34.0	41.0
19	38.68259725850698	40.0	38.0	41.0	34.0	41.0
20	38.65928036090198	40.0	38.0	41.0	34.0	41.0
21	38.59943070666115	40.0	38.0	41.0	34.0	41.0
22	38.538353728595126	40.0	38.0	41.0	34.0	41.0
23	38.5169434957475	40.0	38.0	41.0	34.0	41.0
24	38.46446229460508	40.0	38.0	41.0	34.0	41.0
25	38.41134854530838	40.0	38.0	41.0	34.0	41.0
26	38.21156931701712	40.0	38.0	41.0	33.0	41.0
27	38.10825029964291	40.0	38.0	41.0	33.0	41.0
28	37.99196348133757	40.0	37.0	41.0	33.0	41.0
29	37.8930914691692	40.0	37.0	41.0	33.0	41.0
30	37.834142716845065	40.0	37.0	41.0	33.0	41.0
31	37.827852752712005	40.0	37.0	41.0	33.0	41.0
32	37.74390481785206	40.0	37.0	41.0	33.0	41.0
33	37.705446228332974	40.0	37.0	41.0	32.0	41.0
34	37.618920744309605	40.0	37.0	41.0	32.0	41.0
35	37.55460773557908	40.0	37.0	41.0	32.0	41.0
36	37.5234104831608	40.0	37.0	41.0	32.0	41.0
37	37.34297189388977	40.0	36.0	41.0	31.0	41.0
38	37.248319124490216	40.0	36.0	41.0	31.0	41.0
39	37.20415012476195	39.0	36.0	41.0	31.0	41.0
40	37.09526276681749	39.0	36.0	41.0	31.0	41.0
41	36.96542521104671	39.0	35.0	41.0	31.0	41.0
42	36.89772620925505	39.0	35.0	41.0	31.0	41.0
43	35.98775156755806	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	0.0
11	3.0
12	0.0
13	0.0
14	0.0
15	5.0
16	11.0
17	23.0
18	48.0
19	132.0
20	285.0
21	512.0
22	1012.0
23	1706.0
24	2725.0
25	4273.0
26	6475.0
27	9737.0
28	13567.0
29	18929.0
30	26016.0
31	34200.0
32	44196.0
33	58425.0
34	79807.0
35	106453.0
36	143942.0
37	215306.0
38	400597.0
39	605391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.70666565940044	17.008498244988946	10.913936242303151	27.370899853307463
2	20.43310944210606	18.69681549776804	33.39363776075698	27.476437299368918
3	21.515826670530362	21.268106831858326	27.444358876928227	29.771707620683085
4	15.454470627102152	13.710114794523328	31.65740019325981	39.178014385114714
5	16.00324279588539	35.62858486236722	32.56450356245257	15.803668779294815
6	38.94540353978908	31.54368810527586	14.241748403689753	15.269159951245308
7	31.862499140253174	28.095567765526464	19.88732524588759	20.154607848332766
8	28.95119907902793	31.85314058467294	18.491378289729603	20.704282046569524
9	27.96251842113275	12.61555842952162	17.676507432158928	41.74541571718671
10	18.645174311554207	24.92110061123771	30.808703231182257	25.625021846025824
11	38.51812346302638	19.895274380446708	19.74001256076014	21.846589595766773
12	24.744020954144204	22.92180870435872	26.489673454062455	25.844496887434616
13	31.01109608981507	18.025367323306522	23.757877254087038	27.205659332791367
14	24.00289100439852	18.925479964234533	23.42299881946895	33.648630211897995
15	27.78391658933643	25.369352872794682	20.623663164161467	26.22306737370742
16	27.536704142232004	23.945386626736827	21.109349648039384	27.40855958299178
17	25.78597772663772	24.705571948688053	23.328623987894765	26.17982633677946
18	25.70643000420571	22.614554921754582	24.766797197845502	26.912217876194198
19	26.5141410029891	22.932125666233315	23.607463842713123	26.946269488064463
20	26.117248043441737	23.281943963675275	24.088020034074162	26.512787958808826
21	28.601775419471885	22.736498028501874	22.97660699365986	25.68511955836638
22	27.671050153965155	23.293106578162543	22.992110624892177	26.043732642980128
23	26.66534369013484	23.50598552919249	23.188414784713757	26.640255995958906
24	27.16033235275215	23.51573872265864	23.071094578915737	26.25283434567347
25	26.7725160645808	23.045273985808823	23.672409963366327	26.509799986244047
26	27.265136899882624	23.614905585704637	23.86302006226258	25.256937452150158
27	28.273267567869258	22.798907191317063	22.52390096167615	26.403924279137524
28	26.02862364963372	22.36542566206143	24.594396818542116	27.011553869762732
29	26.145492840704982	22.85799012052241	24.652126703567188	26.34439033520542
30	25.59610052667245	23.405296491443686	24.54072606605787	26.457876915825995
31	25.93481258646798	23.709280417278826	23.688928377733852	26.666978618519344
32	25.577270661830287	23.120649822018315	23.99037534573098	27.311704170420427
33	26.558396823052266	23.293726723411837	23.8481929531204	26.2996835004155
34	26.99559922380366	23.49217320318552	23.72461491798861	25.78761265502222
35	27.104575657156644	23.065682402194636	24.41399092783877	25.415751012809945
36	26.45246473910489	24.05047305807153	22.74782977351168	26.7492324293119
37	25.86124080916552	23.16496201892232	24.128385852119035	26.845411319793122
38	26.607219167223857	22.75391847232292	24.444829059780872	26.19403330067235
39	25.332369665200495	23.22477784705865	24.429776443275316	27.01307604446554
40	26.049483080746295	22.40223973913308	25.350466631111672	26.197810549008953
41	24.43761282415274	22.75735745961445	26.217598820145476	26.587430896087334
42	25.16972247936326	23.30032281379068	25.145480437799993	26.38447426904607
43	25.024890375232978	21.111773852195707	26.320993946254827	27.542341826316484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	88.0
2	80.0
3	206.0
4	332.0
5	332.0
6	467.0
7	602.0
8	584.5
9	567.0
10	811.0
11	1055.0
12	1055.0
13	1937.5
14	2820.0
15	3893.0
16	4966.0
17	5005.5
18	5045.0
19	5045.0
20	6380.0
21	7715.0
22	7891.0
23	8067.0
24	9637.0
25	11207.0
26	11207.0
27	13715.5
28	16224.0
29	19560.5
30	22897.0
31	27551.0
32	32205.0
33	32205.0
34	39092.0
35	45979.0
36	52953.5
37	59928.0
38	68095.0
39	76262.0
40	76262.0
41	81102.5
42	85943.0
43	89717.0
44	93491.0
45	94750.0
46	96009.0
47	96009.0
48	99906.5
49	103804.0
50	110871.0
51	117938.0
52	127283.0
53	136628.0
54	136628.0
55	140018.0
56	143408.0
57	136679.5
58	129951.0
59	121358.5
60	112766.0
61	112766.0
62	111427.5
63	110089.0
64	97646.5
65	85204.0
66	74569.5
67	63935.0
68	63935.0
69	55527.5
70	47120.0
71	43357.5
72	39595.0
73	41200.0
74	42805.0
75	42805.0
76	40495.5
77	38186.0
78	29266.0
79	20346.0
80	12845.5
81	5345.0
82	5345.0
83	3718.5
84	2092.0
85	1736.5
86	1381.0
87	1197.0
88	1013.0
89	1013.0
90	803.5
91	594.0
92	332.0
93	70.0
94	42.5
95	15.0
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1773778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.487205627627446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.13739437501894	41.28074347661956
2	8.628048014450611	8.36699876483419
3	2.4070262099134223	3.501299243734826
4	1.0731963698340077	2.0814517225185942
5	0.6238313700681875	1.5123919958730379
6	0.3702185649344124	1.0770518211084001
7	0.24905939120428913	0.8453335740369853
8	0.18900432533294684	0.7331433269543672
9	0.14542693626798428	0.6346211186359463
>10	0.9012238432243312	8.63103265282794
>50	0.1225490914304511	4.1965011821158225
>100	0.12456344029308353	12.910383963209432
>500	0.019360816772677085	6.3637199852033515
>1k	0.008863988401948545	7.055116417613343
>5k	2.3326285268285646E-4	0.8102107547143734
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	8692	0.4900275006229641	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	5635	0.3176834981604237	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	3510	0.19788271136523286	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	3170	0.1787145854780023	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	3166	0.17848907811462314	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	3088	0.17409168452872908	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	3074	0.17330240875690195	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	3000	0.16913052253438704	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	2808	0.15830616909218628	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	2805	0.1581370385696519	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	2738	0.1543597902330506	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	2542	0.14330992942747064	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	2501	0.14099847895283402	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2417	0.13626282432187117	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	2377	0.13400775068807935	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2330	0.13135803916837394	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	2243	0.12645325401487673	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	2174	0.12256325199658583	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	2144	0.12087194677124194	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	2136	0.12042093204448358	No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG	2037	0.11483962480084882	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	1976	0.11140063750931628	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1930	0.10880730283045567	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	1835	0.10345150295020009	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.6376840844795684E-5	0.0	0.0	6.765220901375482E-4	0.0
3	5.6376840844795684E-5	0.0	0.0	0.0011275368168959137	0.0
4	5.6376840844795684E-5	0.0	0.0	0.00208594311125744	0.0
5	2.2550736337918274E-4	0.0	0.0	0.0024805809971710103	0.0
6	2.2550736337918274E-4	0.0	0.0	0.002536957838015806	0.0
7	2.2550736337918274E-4	0.0	0.0	0.00287521888308458	0.0
8	2.2550736337918274E-4	0.0	0.0	0.003382610450687741	0.0
9	2.2550736337918274E-4	0.0	0.0	0.00575043776616916	0.0
10	2.818842042239784E-4	0.0	0.0	0.007949134559116191	0.0
11	3.946378859135698E-4	0.0	0.0	0.00862565664925374	0.0
12	3.946378859135698E-4	0.0	0.0	0.009189425057701697	0.0
13	3.946378859135698E-4	0.0	0.0	0.009809570306994449	0.0
14	3.946378859135698E-4	0.0	0.0	0.010147831352063224	0.0
15	3.946378859135698E-4	0.0	0.0	0.010880730283045568	0.0
16	3.946378859135698E-4	0.0	0.0	0.011444498691493525	0.0
17	3.946378859135698E-4	0.0	0.0	0.012741166030923824	0.0
18	3.946378859135698E-4	0.0	0.0	0.013868702847819738	0.0
19	3.946378859135698E-4	0.0	0.0	0.01488348598302606	0.0
20	3.946378859135698E-4	5.6376840844795684E-5	0.0	0.016518414367525134	5.6376840844795684E-5
21	4.5101472675836547E-4	5.6376840844795684E-5	0.0	0.01854798063793778	5.6376840844795684E-5
22	4.5101472675836547E-4	5.6376840844795684E-5	0.0	0.021197692157643177	5.6376840844795684E-5
23	4.5101472675836547E-4	5.6376840844795684E-5	0.0	0.024918563653399693	5.6376840844795684E-5
24	4.5101472675836547E-4	5.6376840844795684E-5	0.0	0.031007262464637628	5.6376840844795684E-5
25	4.5101472675836547E-4	5.6376840844795684E-5	0.0	0.033262336098429456	5.6376840844795684E-5
26	4.5101472675836547E-4	5.6376840844795684E-5	0.0	0.03765972968432352	5.6376840844795684E-5
27	5.073915676031611E-4	5.6376840844795684E-5	0.0	0.04713103894624919	5.6376840844795684E-5
28	5.637684084479569E-4	1.1275368168959137E-4	0.0	0.07126032682782174	5.6376840844795684E-5
29	5.637684084479569E-4	1.1275368168959137E-4	0.0	0.10565019974314711	5.6376840844795684E-5
30	5.637684084479569E-4	1.1275368168959137E-4	0.0	0.1566712407076872	5.6376840844795684E-5
31	5.637684084479569E-4	1.1275368168959137E-4	0.0	0.2990791406816411	5.6376840844795684E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGA	40	0.0019315983	23.125002	6
TAATACG	65	2.6842117E-6	22.76923	4
GGTATCA	1375	0.0	19.50909	1
GACAGGC	2130	0.0	18.673708	7
CAGGACA	2425	0.0	18.538143	4
TATACCG	60	9.239511E-4	18.5	5
AACACCG	80	1.617477E-5	18.5	5
TGCATCA	2090	0.0	18.411484	14
GCATCAG	2140	0.0	18.154205	15
ACAGGCT	2185	0.0	18.034325	8
CCAGGAC	2365	0.0	17.913319	3
TCCAGGA	2490	0.0	17.534136	2
GCTGCAT	2215	0.0	17.455982	12
CTGCATC	2280	0.0	16.877193	13
ATCAAGC	2325	0.0	16.550537	30
TATTAGA	325	0.0	16.507692	2
TCTAATA	260	0.0	16.365385	2
ATTAGAG	440	0.0	15.977273	3
GGACAGG	2605	0.0	15.907869	6
ATCAGAA	2485	0.0	15.857143	17
>>END_MODULE