FastQCFastQC Report
Fri 10 Feb 2017
ERR1630562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611845
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74480.46207917014353117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64440.3997903024174161No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63770.3956335751886813No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44550.2763913403584092No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC26210.16260868755990807No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT20800.12904466620549743No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA20260.12569446814054702No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA19570.12141365950199927No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG17550.10888143711088845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1457.2759576E-1219.1379316
AAGACGG2200.017.659095
ACGAACG1655.4569682E-1116.81818215
GGTATCA38350.015.9191651
GGCGATA700.002593100815.85714235
TAGTTCG957.06249E-515.5789487
GCTTTAT6750.015.3481481
ACGGACC2550.015.2352948
TAACGAA1853.0559022E-1015.013
TCCGATA2109.094947E-1214.97619158
CCGATAA2109.094947E-1214.97619159
AATGCCG750.004105359314.85
TTTCGGA2006.184564E-1114.79999930
TCTTATA18150.014.57575937
TATACTG2301.8189894E-1214.4782615
CGAGCCG2600.014.23076915
TACGACG2353.6379788E-1214.1702145
GACGGAC2750.014.1272747
CGCAAGA3150.014.0952392
AATACTG4200.014.0952395
Produced by FastQC (version 0.11.5)