##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630562.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1611845
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.996870666844515	33.0	31.0	34.0	30.0	34.0
2	32.14555617940931	33.0	31.0	34.0	30.0	34.0
3	32.25502638280976	34.0	31.0	34.0	30.0	34.0
4	35.835590891183706	37.0	35.0	37.0	35.0	37.0
5	35.81310299687625	37.0	35.0	37.0	35.0	37.0
6	35.85672443690305	37.0	35.0	37.0	35.0	37.0
7	35.8329101123247	37.0	35.0	37.0	35.0	37.0
8	35.82929562085685	37.0	35.0	37.0	35.0	37.0
9	37.548390819216486	39.0	37.0	39.0	35.0	39.0
10	37.461069767874704	39.0	37.0	39.0	35.0	39.0
11	37.56222589641064	39.0	37.0	39.0	35.0	39.0
12	37.500619476438494	39.0	37.0	39.0	35.0	39.0
13	37.54582233403336	39.0	37.0	39.0	35.0	39.0
14	38.81498655267721	40.0	38.0	41.0	35.0	41.0
15	38.785509772962044	40.0	38.0	41.0	35.0	41.0
16	38.761795954325635	40.0	38.0	41.0	35.0	41.0
17	38.7635101390022	40.0	38.0	41.0	35.0	41.0
18	38.76087527026482	40.0	38.0	41.0	35.0	41.0
19	38.779467008304145	40.0	38.0	41.0	35.0	41.0
20	38.767474540045725	40.0	38.0	41.0	35.0	41.0
21	38.69383222332172	40.0	38.0	41.0	34.0	41.0
22	38.63162214729084	40.0	38.0	41.0	34.0	41.0
23	38.61646126023284	40.0	38.0	41.0	34.0	41.0
24	38.562261880019484	40.0	38.0	41.0	34.0	41.0
25	38.5204197674094	40.0	38.0	41.0	34.0	41.0
26	38.324140348482636	40.0	38.0	41.0	34.0	41.0
27	38.20240593853627	40.0	38.0	41.0	34.0	41.0
28	38.103071945503444	40.0	38.0	41.0	33.0	41.0
29	38.00686046114856	40.0	37.0	41.0	33.0	41.0
30	37.923114195223484	40.0	37.0	41.0	33.0	41.0
31	37.85692296715875	40.0	37.0	41.0	33.0	41.0
32	37.767630262215036	40.0	37.0	41.0	33.0	41.0
33	37.71260449981233	40.0	37.0	41.0	33.0	41.0
34	37.63048121872761	40.0	37.0	41.0	33.0	41.0
35	37.561515530339456	40.0	37.0	41.0	33.0	41.0
36	37.50700036293812	40.0	37.0	41.0	32.0	41.0
37	37.308785894425334	40.0	36.0	41.0	32.0	41.0
38	37.19010512797446	40.0	36.0	41.0	31.0	41.0
39	37.106501555670675	40.0	36.0	41.0	31.0	41.0
40	36.98304303453496	39.0	36.0	41.0	31.0	41.0
41	36.84248237268472	39.0	35.0	41.0	31.0	41.0
42	36.726853388508204	39.0	35.0	41.0	30.0	41.0
43	35.875599700963804	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	3.0
11	7.0
12	3.0
13	1.0
14	2.0
15	3.0
16	8.0
17	16.0
18	38.0
19	102.0
20	199.0
21	408.0
22	761.0
23	1372.0
24	2307.0
25	3783.0
26	5902.0
27	8732.0
28	12625.0
29	17848.0
30	24138.0
31	31388.0
32	40826.0
33	52836.0
34	69530.0
35	92629.0
36	130466.0
37	200033.0
38	354685.0
39	561187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2076006067581	19.40962065210985	12.049669788348135	24.33310895278392
2	19.053134761717164	21.4092546119509	33.46996764577239	26.067642980559548
3	21.514165443947775	23.859986537167035	28.27399656914902	26.351851449736174
4	14.704453592001713	15.141902602297366	34.114260366226276	36.03938343947463
5	14.461129947358462	37.22572579869652	33.73655655475557	14.576587699189439
6	35.199972702089845	33.81565845351135	15.527857827520636	15.456511016878174
7	28.85612450328661	30.255390561747564	21.64500929059556	19.24347564437027
8	28.3684845627216	31.34408085144663	20.327140636971915	19.960293948859846
9	26.937329581938712	13.896063207070158	20.507058681200736	38.65954852979039
10	18.211118314726292	26.60584609562334	31.91622023209428	23.26681535755609
11	34.94746703312043	22.308472588865556	21.256386315061313	21.487674062952703
12	23.034534958386196	25.84423440219128	28.037807605570013	23.083423033852508
13	30.2671782956798	20.04553787740136	25.377254016360133	24.31002981055871
14	23.133862126941487	21.22499371837863	25.610154822579094	30.030989332100795
15	25.93983912845218	27.6399405650047	22.920255979948443	23.49996432659468
16	24.90040915844886	26.27591362693063	24.74698249521511	24.076694719405403
17	23.78938421498345	26.250228775099345	25.604695240547322	24.355691769369884
18	23.154893925904783	24.686120563701845	27.89703724613719	24.261948264256176
19	24.357677071926894	25.111719799360362	27.83146022105103	22.699142907661717
20	24.97268657966492	24.43950876169855	26.962704230245464	23.625100428391068
21	25.074929661350815	24.751759629492913	26.98305358145479	23.190257127701486
22	24.907357717398384	24.962077619125907	27.045466530590723	23.085098132884983
23	24.15114356529319	24.92038626542875	26.749594408891674	24.17887576038639
24	23.592032732675907	25.62243888215058	27.289410582283036	23.496117802890478
25	23.951372495494294	25.57547406853637	26.64803377495975	23.825119661009587
26	23.839326982433175	25.924328952225554	26.828572226237636	23.407771839103635
27	24.096857948500013	24.954322531012597	26.8894341577509	24.059385362736492
28	23.42321997462535	25.547617792033357	27.080829732387418	23.948332500953875
29	23.532907940900024	25.986183535017325	27.421060958094607	23.059847565988044
30	23.615980444769814	25.898147774755014	26.794822082768505	23.691049697706664
31	23.065431229429628	26.52984623211289	26.987768675027684	23.416953863429796
32	22.89010419736389	25.744969274340896	26.778381295968284	24.586545232326927
33	22.478774323833868	26.127884505023747	27.353126386221998	24.040214784920387
34	24.087924086993475	25.083429237923	26.993848664108523	23.834798010975
35	23.29473367476401	25.334507970679564	27.260809817321146	24.10994853723528
36	22.52058975894084	26.23341574406968	27.37372390025096	23.87227059673852
37	23.300565501025222	25.047197466257607	27.713831044548325	23.93840598816884
38	23.194662017749845	25.231954685469137	27.4822330931324	24.091150203648613
39	23.09471444214549	24.980689830597854	27.7719011443408	24.152694582915853
40	22.278072643461375	24.923054015739726	28.31618424848543	24.482689092313468
41	21.46744879315319	25.280718679525638	28.86028123051534	24.391551296805837
42	22.195310343116116	25.05532479860036	29.083069401834543	23.666295456448978
43	22.25307023938406	24.68214995858783	28.574335621601332	24.490444180426778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	721.0
1	634.5
2	548.0
3	1109.5
4	1671.0
5	1671.0
6	2243.0
7	2815.0
8	2650.0
9	2485.0
10	3568.0
11	4651.0
12	4651.0
13	8078.0
14	11505.0
15	14911.5
16	18318.0
17	18226.0
18	18134.0
19	18134.0
20	20403.5
21	22673.0
22	21165.0
23	19657.0
24	21910.5
25	24164.0
26	24164.0
27	27513.0
28	30862.0
29	35005.0
30	39148.0
31	43395.5
32	47643.0
33	47643.0
34	53038.5
35	58434.0
36	63811.5
37	69189.0
38	74294.0
39	79399.0
40	79399.0
41	82236.0
42	85073.0
43	87019.5
44	88966.0
45	91273.0
46	93580.0
47	93580.0
48	98780.5
49	103981.0
50	103686.0
51	103391.0
52	103605.5
53	103820.0
54	103820.0
55	100731.0
56	97642.0
57	96165.0
58	94688.0
59	95990.0
60	97292.0
61	97292.0
62	89576.5
63	81861.0
64	75079.0
65	68297.0
66	59068.0
67	49839.0
68	49839.0
69	44391.0
70	38943.0
71	32594.0
72	26245.0
73	18961.5
74	11678.0
75	11678.0
76	8594.0
77	5510.0
78	4524.0
79	3538.0
80	2886.5
81	2235.0
82	2235.0
83	1710.5
84	1186.0
85	1040.5
86	895.0
87	804.0
88	713.0
89	713.0
90	549.0
91	385.0
92	218.5
93	52.0
94	32.0
95	12.0
96	12.0
97	7.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1611845.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.292691470273375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.75209524409428	52.03057909178125
2	7.977207083956392	9.459801568470109
3	1.7710971406191507	3.1503934896784402
4	0.6749666487017412	1.6008235701678692
5	0.3553000470640884	1.05333480349723
6	0.2273630958103105	0.8088581934964167
7	0.16614029740276032	0.6895633776276929
8	0.11553368338127432	0.5480242434520117
9	0.09418003376317288	0.5025768916121766
>10	0.6674124551439992	7.928165620019155
>50	0.09532970930230808	3.980310816879569
>100	0.0915275125639596	11.14785504684621
>500	0.009016337565565557	3.573261061832694
>1k	0.0025161872275996904	2.266467734498104
>5k	3.145234034499613E-4	1.2599844901410333
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7448	0.46207917014353117	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6444	0.3997903024174161	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6377	0.3956335751886813	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4455	0.2763913403584092	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2621	0.16260868755990807	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2080	0.12904466620549743	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2026	0.12569446814054702	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1957	0.12141365950199927	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1755	0.10888143711088845	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.240814098129783E-4	0.0
2	0.0	0.0	0.0	0.0019232618521011636	0.0
3	0.0	0.0	0.0	0.0024195874913530767	0.0
4	0.0	0.0	0.0	0.004653052867986686	0.0
5	0.0	6.204070490648915E-5	0.0	0.0051493785072386	0.0
6	0.0	6.204070490648915E-5	0.0	0.005459582031771045	0.0
7	0.0	6.204070490648915E-5	0.0	0.006328151900461893	0.0
8	0.0	6.204070490648915E-5	0.0	0.006886518244620296	0.0
9	0.0	6.204070490648915E-5	6.204070490648915E-5	0.010360797719383688	0.0
10	0.0	6.204070490648915E-5	6.204070490648915E-5	0.014765687767744418	0.0
11	0.0	1.240814098129783E-4	6.204070490648915E-5	0.017557519488536427	0.0
12	0.0	1.8612211471946745E-4	6.204070490648915E-5	0.019977106979889504	0.0
13	0.0	1.8612211471946745E-4	6.204070490648915E-5	0.020907717553486842	0.0
14	0.0	1.8612211471946745E-4	6.204070490648915E-5	0.022024450241803648	0.0
15	0.0	1.8612211471946745E-4	6.204070490648915E-5	0.0245681191429697	0.0
16	0.0	2.481628196259566E-4	6.204070490648915E-5	0.028600764961891498	0.0
17	0.0	2.481628196259566E-4	6.204070490648915E-5	0.03275749219062627	0.0
18	0.0	3.1020352453244574E-4	6.204070490648915E-5	0.0354252425016053	0.0
19	0.0	3.1020352453244574E-4	6.204070490648915E-5	0.03877544056655572	0.0
20	0.0	3.1020352453244574E-4	6.204070490648915E-5	0.0423117607462256	0.0
21	0.0	3.1020352453244574E-4	6.204070490648915E-5	0.04944644181047185	0.0
22	0.0	3.1020352453244574E-4	6.204070490648915E-5	0.05937295459551011	0.0
23	0.0	3.722442294389349E-4	6.204070490648915E-5	0.07308395037984422	0.0
24	0.0	5.583663441584023E-4	6.204070490648915E-5	0.09237860960576234	0.0
25	0.0	5.583663441584023E-4	6.204070490648915E-5	0.0995132906700086	0.0
26	0.0	5.583663441584023E-4	6.204070490648915E-5	0.1126659201101843	0.0
27	0.0	5.583663441584023E-4	6.204070490648915E-5	0.13270506779498029	0.0
28	0.0	7.444884588778698E-4	6.204070490648915E-5	0.1732176480989177	0.0
29	0.0	9.306105735973372E-4	6.204070490648915E-5	0.2354444751201263	0.0
30	0.0	9.306105735973372E-4	6.204070490648915E-5	0.31851697898991527	0.0
31	0.0	9.306105735973372E-4	6.204070490648915E-5	0.5991270872819657	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	145	7.2759576E-12	19.13793	16
AAGACGG	220	0.0	17.65909	5
ACGAACG	165	5.4569682E-11	16.818182	15
GGTATCA	3835	0.0	15.919165	1
GGCGATA	70	0.0025931008	15.857142	35
TAGTTCG	95	7.06249E-5	15.578948	7
GCTTTAT	675	0.0	15.348148	1
ACGGACC	255	0.0	15.235294	8
TAACGAA	185	3.0559022E-10	15.0	13
TCCGATA	210	9.094947E-12	14.9761915	8
CCGATAA	210	9.094947E-12	14.9761915	9
AATGCCG	75	0.0041053593	14.8	5
TTTCGGA	200	6.184564E-11	14.799999	30
TCTTATA	1815	0.0	14.575759	37
TATACTG	230	1.8189894E-12	14.478261	5
CGAGCCG	260	0.0	14.230769	15
TACGACG	235	3.6379788E-12	14.170214	5
GACGGAC	275	0.0	14.127274	7
CGCAAGA	315	0.0	14.095239	2
AATACTG	420	0.0	14.095239	5
>>END_MODULE