FastQCFastQC Report
Fri 10 Feb 2017
ERR1630561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735275
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA51070.6945700588215293No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC48600.6609771854068205No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC30360.412906735575125No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC28760.39114616980041483No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG26930.3662575226955901No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC24430.33225663867260546No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC23290.31675223555812454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.311312094114447No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC22880.31117609057835505No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG21230.2887355071231852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20670.28111930910203664No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT20200.27472714290571554No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC19980.2717350651116929No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG18350.24956648872870696No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA17950.2441263472850294No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC17610.2395022270579035No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT17340.23583013158342117No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC16530.22481384515997418No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16420.22331780626296283No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC16100.2189656931080208No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC15680.2132535445921594No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT15590.21202951276733192No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG15560.21162150215905612No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT15210.2068613783958383No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC15190.2065893713236544No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG14370.19543708136411547No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT14300.19448505661147192No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC13680.1860528373737717No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG12540.17054843425929073No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT12520.17027642718710687No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC12430.16905239536227942No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG12400.16864438475400362No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC12270.16687633878480843No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA12130.16497228927952126No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11990.16306823977423412No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC11970.16279623270205026No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG11740.15966815137193568No Hit
GTACATGGGCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA11510.15654007004182108No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG11270.15327598517561458No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT11220.1525959674951549RNA PCR Primer, Index 39 (95% over 23bp)
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC11150.1516439427425113No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC11090.15082792152595967No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG11070.15055591445377578No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT11030.15001190030940803No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC10930.14865186494848867No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG10720.14579579069055795No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG10560.14361973411308693No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA10520.14307571996871918No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAAC10450.14212369521607562No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA10210.1388596103498691No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC10190.13858760327768524No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG10050.13668355377239808No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC9950.1353235184114787No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCA9860.13409948658665125No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT9650.13124341232872055No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAG9570.13015538403998503No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC9410.12797932746251403No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA9200.12512325320458334No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA9100.12376321784366394No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG8930.12145115773010098No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA8690.11818707286389446No Hit
ACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCG8240.11206691373975725No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG8120.11043487130665397No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG8070.10975485362619429No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT7990.10866682533745876No Hit
TCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA7860.10689877936826356No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC7830.10649076875998775No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGG7800.10608275815171195No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG7780.10581075107952806No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACC7750.10540274047125225No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA7690.10458671925470062No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG7550.1026826697494135No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG7480.10173064499676991No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG7370.1002346060997586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG3550.026.5774655
GTGCTAG451.3225788E-424.6666661
AGACGGA3800.024.3421066
GCCGTCT1750.024.31428736
CGCAAGA3950.023.8860762
GCGCAAG4050.023.7530861
ACGGACC4000.023.5874988
TAGTACC551.9014498E-523.5454544
TATTAGA952.8012437E-1023.3684222
AGTCGGT952.8012437E-1023.36842211
GTAGGCT400.001930529323.1254
CAAGACG4250.023.070594
GACGGAC4050.022.8395067
CGGACCA4150.022.734949
CAGTACG753.7369682E-722.237
GAATCTA502.70082E-422.19999913
TACCGTT1107.4578566E-1121.86363618
ATTTCGG855.1742973E-821.76470629
GGTATCA12850.021.1634251
TTTCGGT806.953105E-720.812530
Produced by FastQC (version 0.11.5)