FastQCFastQC Report
Fri 10 Feb 2017
ERR1630560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692257
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122111.7639402707376017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT120631.7425609275168035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94721.3682779661310756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62340.9005326056652372No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.25294074310552295No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16200.2340171352546814No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16170.233583770189395No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA15520.22419419377485528No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC13710.19804783483590632No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT13240.19125844881308532No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12530.18100214226797273No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11340.163811994678277No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA11160.16121180428655832No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC10760.15543360341607237No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10550.15240004795906723No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG10140.14647739206681912No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9760.14098810123985744No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9080.13116515976003132No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC8900.12856496936831263No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC8710.1258203239548318No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT8660.12509804884602105No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC8650.12495359382425891No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT8580.12394240867192385RNA PCR Primer, Index 47 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8540.12336458858487527No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8410.12148667330196734No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT8010.11570847243148137No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.11440837723562204No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7910.11426392221385988No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7780.11238600693095195No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC7750.11195264186566549No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG7520.10863017636513607No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC7490.10819681129984962No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7400.10689671610399029No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA7250.10472989077755805No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG7210.10415207069050945No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC7140.10314088553817441No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7140.10314088553817441No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7100.10256306545112581No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT7040.10169633532055292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCA251.2331188E-436.99999618
TCCATTA250.00549485329.59999821
CGGTTAT250.00549485329.59999814
AGAACCG250.00549485329.5999985
TGCGGTA405.9367692E-527.7536
TAGTGTC556.2557046E-726.9090919
AACTTAT451.3224693E-424.66666810
CCGTCTT1400.023.78571537
GGTAAAC1700.022.85294235
CCGGACC502.7005986E-422.19999934
GCAGCGT502.7005986E-422.1999991
TTGGTAA1850.022.033
GCAGTCG3900.021.8205139
TCTATAC603.7235302E-521.5833343
CTAGTGT705.0970866E-621.14285918
GTAAACA1850.021.036
GCCGTCT1600.020.812536
TCTTGGA450.003824293420.5555572
CGATGCA450.003824293420.55555734
GAACCGC450.003824293420.5555576
Produced by FastQC (version 0.11.5)