##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630560.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692257
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.859660501807856	33.0	31.0	34.0	30.0	34.0
2	31.964924876165934	33.0	31.0	34.0	30.0	34.0
3	32.002682529754125	33.0	31.0	34.0	30.0	34.0
4	35.64941488493435	37.0	35.0	37.0	33.0	37.0
5	35.665866867362844	37.0	35.0	37.0	33.0	37.0
6	35.74925063957461	37.0	35.0	37.0	35.0	37.0
7	35.71492379275327	37.0	35.0	37.0	33.0	37.0
8	35.747169042711015	37.0	35.0	37.0	35.0	37.0
9	37.38687943928339	39.0	37.0	39.0	34.0	39.0
10	37.284304817430524	39.0	37.0	39.0	34.0	39.0
11	37.439354170488706	39.0	37.0	39.0	35.0	39.0
12	37.38791229268899	39.0	37.0	39.0	34.0	39.0
13	37.444918577926984	39.0	37.0	39.0	35.0	39.0
14	38.56678372338597	40.0	38.0	41.0	34.0	41.0
15	38.510875296313365	40.0	38.0	41.0	34.0	41.0
16	38.436869544114394	40.0	38.0	41.0	34.0	41.0
17	38.51916845911273	40.0	38.0	41.0	34.0	41.0
18	38.56637924932503	40.0	38.0	41.0	34.0	41.0
19	38.63286178399063	40.0	38.0	41.0	34.0	41.0
20	38.616078999562305	40.0	38.0	41.0	34.0	41.0
21	38.54390060338863	40.0	38.0	41.0	34.0	41.0
22	38.44498936088765	40.0	38.0	41.0	34.0	41.0
23	38.374066278853086	40.0	38.0	41.0	34.0	41.0
24	38.30800410830082	40.0	38.0	41.0	34.0	41.0
25	38.22354125707649	40.0	37.0	41.0	34.0	41.0
26	37.93563373140322	40.0	37.0	41.0	33.0	41.0
27	37.74131861432965	40.0	36.0	41.0	33.0	41.0
28	37.57301262392435	40.0	36.0	41.0	33.0	41.0
29	37.398604853399824	39.0	36.0	41.0	32.0	41.0
30	37.18179231123701	39.0	35.0	41.0	32.0	41.0
31	37.01089768684174	39.0	35.0	41.0	32.0	41.0
32	36.781702460213474	39.0	35.0	41.0	31.0	41.0
33	36.61803492055696	39.0	35.0	41.0	31.0	41.0
34	36.42234314712599	39.0	35.0	41.0	30.0	41.0
35	36.2146067139805	39.0	35.0	41.0	30.0	41.0
36	36.04965785828096	38.0	35.0	40.0	30.0	41.0
37	35.70202684841035	38.0	35.0	40.0	28.0	41.0
38	35.421418634986715	38.0	35.0	40.0	26.0	41.0
39	35.15536859865628	38.0	35.0	40.0	25.0	41.0
40	34.8524666417241	38.0	34.0	40.0	23.0	41.0
41	34.51213783320357	38.0	34.0	40.0	21.0	41.0
42	34.16521465293959	38.0	33.0	40.0	18.0	41.0
43	33.24070251366183	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	5.0
11	11.0
12	2.0
13	3.0
14	1.0
15	4.0
16	8.0
17	19.0
18	30.0
19	103.0
20	171.0
21	381.0
22	664.0
23	1114.0
24	1858.0
25	2896.0
26	4378.0
27	6395.0
28	8825.0
29	11873.0
30	15271.0
31	18644.0
32	22859.0
33	29049.0
34	36818.0
35	47220.0
36	65048.0
37	104295.0
38	146319.0
39	167988.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.845690256653235	20.274406759339378	13.156674472053009	20.723228511954375
2	19.494638551867297	22.53715022022168	33.284026019238524	24.684185208672503
3	22.66094817387184	26.470371552761474	28.869913919252532	21.998766354114153
4	13.634676139063961	16.835654966291422	32.70880612258164	36.82086277206298
5	12.473691129161569	41.37004031739657	31.532942245437752	14.623326308004108
6	31.330849669992507	33.22711074066424	17.367104991354367	18.074934597988896
7	26.86690058749857	33.53277756671294	21.220731606903218	18.379590238885267
8	31.05566285353561	29.945959376358783	19.395542406938464	19.60283536316715
9	28.795086217979737	12.239529538885124	22.17153456014168	36.793849682993454
10	20.98093627077805	28.455905826882212	29.057560992521562	21.505596909818177
11	32.17345581193112	24.18379301328842	19.650216610305133	23.99253456447533
12	22.403095960026405	30.363867754316676	26.393232571140484	20.83980371451643
13	29.780847286484647	20.532836793271862	28.33889725925487	21.34741866098862
14	22.947980302113233	22.773478635824556	28.33413024353672	25.944410818525487
15	27.192502206550458	26.16759382714801	26.631727812069794	20.008176154231737
16	23.30001719014759	25.50887892791261	28.672443904503673	22.518659977436126
17	21.84001028519755	27.840382979153695	27.846883455132993	22.472723280515762
18	20.327421752326085	23.70478016112513	32.068437011110035	23.899361075438748
19	21.021672586914974	26.20587440791498	32.65116856889855	20.121284436271498
20	22.068249219581745	23.41240319707854	34.206371333189836	20.312976250149873
21	22.99825064968646	25.281651178680747	32.042724017236374	19.677374154396414
22	23.83623423092869	24.926436280167625	30.81037822658348	20.42695126232021
23	21.753481727162022	25.006753272267378	31.8861347736462	21.353630226924395
24	20.192934127065527	27.228615961990993	31.063318969689004	21.515130941254476
25	20.510735174942255	26.361741376396335	32.05572496919496	21.07179847946644
26	20.95146744633857	27.4980245775774	31.24663239230517	20.303875583778858
27	21.311015995504558	27.059892496572807	31.144791601962858	20.48429990595978
28	19.96657310796424	26.92439368615991	32.43983087206052	20.66920233381533
29	19.958772536789084	27.42608597673985	32.76471021600359	19.85043127046747
30	18.200466011900204	28.448538620772347	33.49839727153355	19.8525980957939
31	20.496867492853088	28.684433671309932	30.73410597509307	20.084592860743914
32	18.8252339810215	27.992349662047477	31.708021731813474	21.47439462511755
33	18.528378911300283	28.09000125675869	32.03593463121355	21.345685200727477
34	20.090082151570876	27.738397733789615	31.84063144179113	20.33088867284838
35	18.80255454260484	29.14553410077471	31.673063616547033	20.37884774007341
36	19.45390223573037	29.036903924409575	30.576505546350564	20.93268829350949
37	19.39929823750428	27.636123578382016	31.695165234876644	21.26941294923706
38	19.07470780360473	26.943028383967228	31.555621683854408	22.426642128573636
39	19.464014087253723	28.09086798688926	31.73330136062185	20.711816565235168
40	18.38363497949461	28.103291118760808	31.40928874680935	22.103785154935235
41	18.781464109427567	27.769744473512002	31.42330088392028	22.02549053314015
42	18.2390355027107	28.61177279536357	31.94420569239459	21.204986009531144
43	17.845828933474127	26.87527897876078	31.459270184339054	23.81962190342604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	546.0
1	412.5
2	279.0
3	838.5
4	1398.0
5	1398.0
6	2147.5
7	2897.0
8	3022.5
9	3148.0
10	4640.0
11	6132.0
12	6132.0
13	11058.0
14	15984.0
15	22127.0
16	28270.0
17	25496.0
18	22722.0
19	22722.0
20	23833.0
21	24944.0
22	17972.5
23	11001.0
24	10037.0
25	9073.0
26	9073.0
27	9739.0
28	10405.0
29	11472.0
30	12539.0
31	14175.5
32	15812.0
33	15812.0
34	18617.0
35	21422.0
36	23318.5
37	25215.0
38	29456.0
39	33697.0
40	33697.0
41	35631.0
42	37565.0
43	40497.5
44	43430.0
45	43612.5
46	43795.0
47	43795.0
48	47802.0
49	51809.0
50	49639.5
51	47470.0
52	46431.0
53	45392.0
54	45392.0
55	40359.0
56	35326.0
57	32309.0
58	29292.0
59	27960.0
60	26628.0
61	26628.0
62	24928.0
63	23228.0
64	21355.5
65	19483.0
66	18279.0
67	17075.0
68	17075.0
69	13568.5
70	10062.0
71	8767.5
72	7473.0
73	5484.5
74	3496.0
75	3496.0
76	2795.5
77	2095.0
78	1618.5
79	1142.0
80	918.5
81	695.0
82	695.0
83	599.5
84	504.0
85	434.5
86	365.0
87	318.0
88	271.0
89	271.0
90	202.5
91	134.0
92	86.0
93	38.0
94	20.0
95	2.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	692257.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.16022818909772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.04445502813702	25.420877107877597
2	9.373606501693333	6.029146480985352
3	3.2626658970431524	3.1478423926108503
4	1.708105743594054	2.197322819403729
5	1.0955276107474716	1.761620897454786
6	0.7636052546052186	1.4734631540698737
7	0.5863681385972805	1.3200413198073513
8	0.44573177978573	1.1467868599233388
9	0.3476291685447427	1.0061850047026715
>10	2.722365220638639	17.715581192521224
>50	0.3452522725153717	7.806583190376626
>100	0.27133403140086415	17.297519037247813
>500	0.026141816978820797	5.579526889532923
>1k	0.005408651788721545	2.302286854059322
>5k	9.014419647869242E-4	2.276630191390525
>10k+	9.014419647869242E-4	3.5185866080360126
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12211	1.7639402707376017	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12063	1.7425609275168035	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9472	1.3682779661310756	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6234	0.9005326056652372	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1751	0.25294074310552295	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1620	0.2340171352546814	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1617	0.233583770189395	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1552	0.22419419377485528	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1371	0.19804783483590632	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	1324	0.19125844881308532	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1253	0.18100214226797273	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1134	0.163811994678277	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1116	0.16121180428655832	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1076	0.15543360341607237	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1055	0.15240004795906723	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1014	0.14647739206681912	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	976	0.14098810123985744	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	908	0.13116515976003132	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	890	0.12856496936831263	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	871	0.1258203239548318	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	866	0.12509804884602105	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	865	0.12495359382425891	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	858	0.12394240867192385	RNA PCR Primer, Index 47 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	854	0.12336458858487527	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	841	0.12148667330196734	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	801	0.11570847243148137	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	792	0.11440837723562204	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	791	0.11426392221385988	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	778	0.11238600693095195	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	775	0.11195264186566549	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	752	0.10863017636513607	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	749	0.10819681129984962	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	740	0.10689671610399029	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	725	0.10472989077755805	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	721	0.10415207069050945	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	714	0.10314088553817441	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	714	0.10314088553817441	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	710	0.10256306545112581	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	704	0.10169633532055292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.333650652864471E-4	0.0
3	0.0	0.0	0.0	0.0010111851523350432	0.0
4	0.0	0.0	0.0	0.0013000951958593414	0.0
5	0.0	0.0	0.0	0.0015890052393836393	0.0
6	0.0	0.0	0.0	0.0015890052393836393	0.0
7	0.0	0.0	0.0	0.0017334602611457884	0.0
8	0.0	0.0	0.0	0.0018779152829079373	0.0
9	0.0	0.0	0.0	0.003900285587578024	0.0
10	0.0	0.0	0.0	0.005778200870485961	0.0
11	0.0	0.0	0.0	0.007367206109869601	0.0
12	0.0	0.0	0.0	0.010834126632161177	0.0
13	0.0	0.0	0.0	0.011556401740971922	0.0
14	0.0	0.0	0.0	0.013578772045642009	0.0
15	0.0	0.0	0.0	0.018345787763792927	0.0
16	0.0	0.0	0.0	0.029324369417716253	0.0
17	0.0	0.0	0.0	0.04535887683331479	0.0
18	0.0	0.0	0.0	0.05330390303023299	0.0
19	0.0	0.0	0.0	0.06341575455358342	0.0
20	0.0	0.0	0.0	0.07179414581578807	0.0
21	0.0	0.0	0.0	0.09577367942830481	0.0
22	0.0	0.0	0.0	0.13362089512998784	0.0
23	0.0	0.0	0.0	0.18215778244206993	0.0
24	0.0	0.0	0.0	0.26117467934596544	0.0
25	0.0	0.0	0.0	0.2803871972403313	0.0
26	0.0	0.0	0.0	0.30884483652747463	0.0
27	0.0	0.0	0.0	0.34611423214210907	0.0
28	0.0	0.0	0.0	0.4098188967392168	0.0
29	0.0	0.0	0.0	0.5042924809716622	0.0
30	0.0	0.0	0.0	0.6720047612375173	0.0
31	0.0	0.0	0.0	1.125449074548903	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCA	25	1.2331188E-4	36.999996	18
TCCATTA	25	0.005494853	29.599998	21
CGGTTAT	25	0.005494853	29.599998	14
AGAACCG	25	0.005494853	29.599998	5
TGCGGTA	40	5.9367692E-5	27.75	36
TAGTGTC	55	6.2557046E-7	26.90909	19
AACTTAT	45	1.3224693E-4	24.666668	10
CCGTCTT	140	0.0	23.785715	37
GGTAAAC	170	0.0	22.852942	35
CCGGACC	50	2.7005986E-4	22.199999	34
GCAGCGT	50	2.7005986E-4	22.199999	1
TTGGTAA	185	0.0	22.0	33
GCAGTCG	390	0.0	21.820513	9
TCTATAC	60	3.7235302E-5	21.583334	3
CTAGTGT	70	5.0970866E-6	21.142859	18
GTAAACA	185	0.0	21.0	36
GCCGTCT	160	0.0	20.8125	36
TCTTGGA	45	0.0038242934	20.555557	2
CGATGCA	45	0.0038242934	20.555557	34
GAACCGC	45	0.0038242934	20.555557	6
>>END_MODULE