FastQCFastQC Report
Fri 10 Feb 2017
ERR1630559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077866
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC21850.2027153653608148No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA20980.19464386111075033No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA19270.1787791803433822No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT17360.16105898135760846No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA15730.1459365078776026No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC13790.12793798115906801No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG12590.11680487184863425No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA12160.11281550767906215No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG12020.1115166449261782No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG11520.10687784938016413No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11260.10446567569623681No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC11220.10409457205255569No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG11210.10400179614163542No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA10790.1001052078829836No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG10780.10001243197206332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8300.022.734941
CAAGACG2550.022.4901984
AAGACGG2500.022.1999995
TTAGAAC759.264897E-619.7333323
GACGGAC2850.019.4736847
GCTTTAT5850.018.974361
ACGGACC2850.018.824568
CGCAATT609.2363084E-418.512
TATACTG1205.171387E-918.55
GTAATAC1002.8745126E-718.4999983
GCGCAAG3250.017.6461541
GCGAAAG3050.017.59016418
CGGACCA3050.017.5901649
GACGCAA752.0671164E-417.26666610
CGCAAGA3350.017.1194022
GTACTAG650.001579847717.0769231
TCTATAC1301.3928002E-817.0769233
TTAGAGT2200.016.8181824
ACCTAGG5950.016.7899151
CTTTATT7300.016.4726032
Produced by FastQC (version 0.11.5)