##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630559.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1077866
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.006438648217866	33.0	31.0	34.0	30.0	34.0
2	32.16944870698213	33.0	31.0	34.0	30.0	34.0
3	32.28403437904155	34.0	31.0	34.0	30.0	34.0
4	35.87068986311842	37.0	35.0	37.0	35.0	37.0
5	35.8413615421583	37.0	35.0	37.0	35.0	37.0
6	35.87409845008563	37.0	35.0	37.0	35.0	37.0
7	35.85002402896093	37.0	35.0	37.0	35.0	37.0
8	35.835693861760184	37.0	35.0	37.0	35.0	37.0
9	37.57904414834497	39.0	37.0	39.0	35.0	39.0
10	37.48104495363988	39.0	37.0	39.0	35.0	39.0
11	37.58785229332774	39.0	37.0	39.0	35.0	39.0
12	37.50624474656404	39.0	37.0	39.0	35.0	39.0
13	37.55953894083309	39.0	37.0	39.0	35.0	39.0
14	38.82414047757328	40.0	38.0	41.0	35.0	41.0
15	38.81294335288431	40.0	38.0	41.0	35.0	41.0
16	38.79091927939094	40.0	38.0	41.0	35.0	41.0
17	38.77236224168867	40.0	38.0	41.0	35.0	41.0
18	38.75772962501832	40.0	38.0	41.0	35.0	41.0
19	38.75973172917598	40.0	38.0	41.0	35.0	41.0
20	38.75213430983072	40.0	38.0	41.0	35.0	41.0
21	38.66949045614204	40.0	38.0	41.0	34.0	41.0
22	38.612862823393634	40.0	38.0	41.0	34.0	41.0
23	38.60172321976943	40.0	38.0	41.0	34.0	41.0
24	38.550663997194455	40.0	38.0	41.0	34.0	41.0
25	38.50609723286568	40.0	38.0	41.0	34.0	41.0
26	38.3367951118228	40.0	38.0	41.0	34.0	41.0
27	38.22601789090666	40.0	38.0	41.0	34.0	41.0
28	38.114209929620195	40.0	38.0	41.0	33.0	41.0
29	38.03565656584399	40.0	38.0	41.0	33.0	41.0
30	37.97436694357184	40.0	37.0	41.0	33.0	41.0
31	37.949757205441124	40.0	37.0	41.0	33.0	41.0
32	37.88573533259236	40.0	37.0	41.0	33.0	41.0
33	37.85715942426981	40.0	37.0	41.0	33.0	41.0
34	37.80635626320897	40.0	37.0	41.0	33.0	41.0
35	37.75367717322933	40.0	37.0	41.0	33.0	41.0
36	37.727448495453054	40.0	37.0	41.0	33.0	41.0
37	37.55746632698313	40.0	37.0	41.0	32.0	41.0
38	37.46664056571039	40.0	37.0	41.0	32.0	41.0
39	37.42430135100282	40.0	36.0	41.0	32.0	41.0
40	37.32421840933845	40.0	36.0	41.0	31.0	41.0
41	37.2318080355072	39.0	36.0	41.0	31.0	41.0
42	37.164346031881514	39.0	36.0	41.0	31.0	41.0
43	36.25819350457292	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	1.0
13	1.0
14	2.0
15	2.0
16	7.0
17	11.0
18	25.0
19	55.0
20	131.0
21	275.0
22	500.0
23	847.0
24	1390.0
25	2230.0
26	3483.0
27	5155.0
28	7604.0
29	10657.0
30	14725.0
31	19400.0
32	25484.0
33	33903.0
34	44947.0
35	61814.0
36	86636.0
37	132238.0
38	247786.0
39	378552.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.54950800934439	18.092972595851435	11.416261390562463	25.941258004241714
2	20.635774762354504	19.598076198711155	31.76192587946925	28.00422315946509
3	21.764857598254327	21.093809434567934	27.967020019186055	29.17431294799168
4	15.103176090534445	13.929560817392886	33.08500314510338	37.882259946969285
5	15.737299441674569	35.38714459867924	33.60000222662186	15.275553733024328
6	38.34372732788677	32.67678913705414	14.050726157054774	14.928757378004315
7	31.36614384348333	28.51504732499216	19.908318844828578	20.210489986695933
8	29.667602466354815	30.16441746933292	18.956716326519253	21.21126373779301
9	27.314712589505557	12.888707872778248	18.487177441351708	41.30940209636449
10	18.41100841848616	24.95004017196943	31.57099305479531	25.067958354749102
11	38.42926671775527	20.10351936140485	19.76423785516938	21.7029760656705
12	24.433556675876222	23.95752347694426	26.26912807343399	25.339791773745528
13	32.344558600048614	17.93831515234732	23.304937719531	26.41218852807306
14	24.252921977314436	19.711077258212057	22.814617030317315	33.2213837341562
15	27.660395633594533	26.547826909838513	20.30716248587487	25.48461497069209
16	27.639428277726548	24.516962219793555	21.834625083266378	26.008984419213522
17	26.329710743264933	24.180278439063855	22.906929061682995	26.583081755988218
18	25.743366986248756	22.88596170581501	24.959132211239616	26.41153909669662
19	26.90352975230687	23.382220053327593	24.29763996637801	25.416610227987523
20	27.777386057265	22.518847426303452	23.11020108250933	26.593565433922212
21	27.913209990852295	23.009817546893586	23.34844962175261	25.728522840501512
22	27.590628148582475	23.0817188778568	23.65386792050218	25.673785053058545
23	26.64505606448297	22.79782459044074	23.425824731460125	27.131294613616163
24	26.160580257657262	23.73764456806319	24.01012741843606	26.09164775584349
25	26.77048909604719	23.570462376584842	23.25632314220877	26.402725385159194
26	26.79813631750143	24.099285068830447	23.418402658586505	25.684175955081617
27	27.217112331217425	22.387569512351256	23.531867597642005	26.86345055878931
28	25.904147639873603	23.325812299488064	23.82559613161562	26.944443929022714
29	25.876129314775675	23.87643733079993	24.41676423599965	25.830669118424737
30	26.10417250381773	24.10429496802014	23.584100435490125	26.207432092672
31	25.935413121853735	24.63367431573127	23.579739967676872	25.85117259473812
32	25.615336229178766	23.38444667518968	23.41496994988245	27.585247145749104
33	24.75994233049377	23.93535003423431	24.254128064156397	27.05057957111552
34	27.190392868872383	22.499735588653877	23.91234160832608	26.397529934147656
35	26.022900805851563	22.831595022015723	24.48504730643698	26.660456865695735
36	24.852532689592213	24.41073380178983	24.563628502986457	26.1731050056315
37	26.196113431539725	22.74475676939434	24.985202242208217	26.07392755685772
38	25.7655404289587	23.01900236207469	24.767828282921997	26.447628926044608
39	25.778529056487542	22.43776128015913	25.052372001714502	26.731337661638833
40	24.79167169202851	22.422824358500964	25.83948282996217	26.946021119508362
41	23.784218075345173	22.805896094690805	26.579741823195093	26.83014400676893
42	24.9009617150926	22.43071031092919	27.047703517876993	25.620624456101222
43	24.54813492586277	22.016558644581053	26.50784049223187	26.927465937324307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	23.0
2	26.0
3	88.0
4	150.0
5	150.0
6	216.5
7	283.0
8	279.5
9	276.0
10	373.5
11	471.0
12	471.0
13	794.5
14	1118.0
15	1684.5
16	2251.0
17	2341.0
18	2431.0
19	2431.0
20	3070.0
21	3709.0
22	4080.0
23	4451.0
24	5692.0
25	6933.0
26	6933.0
27	8531.0
28	10129.0
29	12293.0
30	14457.0
31	17333.0
32	20209.0
33	20209.0
34	23696.5
35	27184.0
36	31684.5
37	36185.0
38	40503.0
39	44821.0
40	44821.0
41	47878.5
42	50936.0
43	52747.5
44	54559.0
45	57789.0
46	61019.0
47	61019.0
48	66316.0
49	71613.0
50	73296.0
51	74979.0
52	78564.0
53	82149.0
54	82149.0
55	80729.0
56	79309.0
57	79408.5
58	79508.0
59	81176.0
60	82844.0
61	82844.0
62	78276.0
63	73708.0
64	68375.0
65	63042.0
66	55127.5
67	47213.0
68	47213.0
69	41020.0
70	34827.0
71	29339.5
72	23852.0
73	17387.5
74	10923.0
75	10923.0
76	7906.0
77	4889.0
78	3895.5
79	2902.0
80	2392.0
81	1882.0
82	1882.0
83	1446.5
84	1011.0
85	866.5
86	722.0
87	626.0
88	530.0
89	530.0
90	416.0
91	302.0
92	167.0
93	32.0
94	20.5
95	9.0
96	9.0
97	5.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1077866.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.07283472617984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.11139485927764	50.858399110229044
2	6.79382566575357	7.754857787600644
3	1.5231772352458406	2.6079612781759622
4	0.6465729806927181	1.4760701146195587
5	0.36478510652732954	1.0409660047703093
6	0.22466027365899907	0.7693199200847032
7	0.16350666063078684	0.6532252033167332
8	0.12804299992944657	0.5846221578254049
9	0.10167145499314859	0.5222410332474782
>10	0.6996985497594358	8.130687138776564
>50	0.10405190111900449	4.211615971454177
>100	0.12360956828328053	14.387591616625569
>500	0.012067424625351245	4.734592369858379
>1k	0.0029353195034638162	2.2678502934154845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2185	0.2027153653608148	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2098	0.19464386111075033	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1927	0.1787791803433822	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1736	0.16105898135760846	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1573	0.1459365078776026	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1379	0.12793798115906801	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1259	0.11680487184863425	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	1216	0.11281550767906215	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	1202	0.1115166449261782	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	1152	0.10687784938016413	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1126	0.10446567569623681	No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC	1122	0.10409457205255569	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	1121	0.10400179614163542	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	1079	0.1001052078829836	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	1078	0.10001243197206332	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.494313764419696E-4	0.0
3	0.0	0.0	0.0	0.0012060868419636577	0.0
4	0.0	0.0	0.0	0.0021338459511664716	0.0
5	9.277591092028138E-5	0.0	0.0	0.0021338459511664716	0.0
6	1.8555182184056275E-4	0.0	0.0	0.0021338459511664716	0.0
7	1.8555182184056275E-4	0.0	0.0	0.002319397773007034	0.0
8	1.8555182184056275E-4	0.0	0.0	0.002504949594847597	0.0
9	1.8555182184056275E-4	0.0	0.0	0.005288226922456038	0.0
10	1.8555182184056275E-4	0.0	0.0	0.008257056071905041	0.0
11	1.8555182184056275E-4	0.0	0.0	0.0094631429138687	0.0
12	1.8555182184056275E-4	0.0	0.0	0.010762005666752639	0.0
13	1.8555182184056275E-4	0.0	0.0	0.011225885221354046	0.0
14	1.8555182184056275E-4	0.0	0.0	0.011596988865035171	0.0
15	1.8555182184056275E-4	0.0	0.0	0.012524747974237985	0.0
16	1.8555182184056275E-4	0.0	0.0	0.01419471437080305	0.0
17	1.8555182184056275E-4	0.0	0.0	0.01623578441104924	0.0
18	1.8555182184056275E-4	0.0	0.0	0.017349095342092617	0.0
19	1.8555182184056275E-4	0.0	0.0	0.018555182184056274	0.0
20	1.8555182184056275E-4	0.0	0.0	0.019390165382338807	0.0
21	1.8555182184056275E-4	0.0	0.0	0.02180233906626612	0.0
22	1.8555182184056275E-4	0.0	0.0	0.025142271859396253	0.0
23	1.8555182184056275E-4	0.0	0.0	0.0298738433163306	0.0
24	1.8555182184056275E-4	0.0	0.0	0.036275381169830014	0.0
25	1.8555182184056275E-4	0.0	0.0	0.03961531396296015	0.0
26	1.8555182184056275E-4	0.0	0.0	0.04443966133081478	0.0
27	1.8555182184056275E-4	0.0	0.0	0.05019176780787222	0.0
28	2.783277327608441E-4	0.0	0.0	0.07514848784542791	0.0
29	2.783277327608441E-4	0.0	0.0	0.11513490545206918	0.0
30	2.783277327608441E-4	0.0	0.0	0.16894493378583236	0.0
31	2.783277327608441E-4	0.0	0.0	0.35876444752872805	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	830	0.0	22.73494	1
CAAGACG	255	0.0	22.490198	4
AAGACGG	250	0.0	22.199999	5
TTAGAAC	75	9.264897E-6	19.733332	3
GACGGAC	285	0.0	19.473684	7
GCTTTAT	585	0.0	18.97436	1
ACGGACC	285	0.0	18.82456	8
CGCAATT	60	9.2363084E-4	18.5	12
TATACTG	120	5.171387E-9	18.5	5
GTAATAC	100	2.8745126E-7	18.499998	3
GCGCAAG	325	0.0	17.646154	1
GCGAAAG	305	0.0	17.590164	18
CGGACCA	305	0.0	17.590164	9
GACGCAA	75	2.0671164E-4	17.266666	10
CGCAAGA	335	0.0	17.119402	2
GTACTAG	65	0.0015798477	17.076923	1
TCTATAC	130	1.3928002E-8	17.076923	3
TTAGAGT	220	0.0	16.818182	4
ACCTAGG	595	0.0	16.789915	1
CTTTATT	730	0.0	16.472603	2
>>END_MODULE