FastQCFastQC Report
Fri 10 Feb 2017
ERR1630558.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630558.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627505
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58210.9276420108206309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56190.8954510322626912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44030.7016677157950932No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32350.5155337407670059No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.15250874495023944No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.1459749324706576No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8200.1306762495916367No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6970.11107481215289121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGA250.00549446529.625
GTATTGA450.00382390220.5555551
CGGGGTA450.00382390220.55555530
TAGAACT1355.638867E-1119.1851864
TAGGCCG500.00703140918.55
TCACGTA1205.160473E-918.525
ATCACGT1258.556526E-917.7624
TCTTGTA1054.7886897E-717.6190472
CCTAGAA852.7199263E-517.4117642
GCCGCAC752.0653814E-417.26666612
CTAGGAC752.0653814E-417.2666663
GCCTACA650.001578853517.0769231
AGTCGGT1757.2759576E-1216.91428611
ATAGGCC803.3795042E-416.18753
GTTTAAG700.002590734715.8571431
TTAGAAC700.002590734715.8571433
ATAAGAC700.002590734715.8571433
TAGAGGT1059.329502E-615.8571424
CAGTCGG1902.7284841E-1115.57894810
TTAATCG1455.331276E-815.31034537
Produced by FastQC (version 0.11.5)