##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630558.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	627505
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97222810973618	33.0	31.0	34.0	30.0	34.0
2	32.105675651986836	33.0	31.0	34.0	30.0	34.0
3	32.188342722368745	34.0	31.0	34.0	30.0	34.0
4	35.790506848551004	37.0	35.0	37.0	35.0	37.0
5	35.781981020071555	37.0	35.0	37.0	35.0	37.0
6	35.83375749994024	37.0	35.0	37.0	35.0	37.0
7	35.79732113688337	37.0	35.0	37.0	35.0	37.0
8	35.81596003219098	37.0	35.0	37.0	35.0	37.0
9	37.510552107154524	39.0	37.0	39.0	35.0	39.0
10	37.41977354762113	39.0	37.0	39.0	34.0	39.0
11	37.54828726464331	39.0	37.0	39.0	35.0	39.0
12	37.47927586234373	39.0	37.0	39.0	35.0	39.0
13	37.53168500649397	39.0	37.0	39.0	35.0	39.0
14	38.76700105975251	40.0	38.0	41.0	35.0	41.0
15	38.72952566115011	40.0	38.0	41.0	35.0	41.0
16	38.68462721412578	40.0	38.0	41.0	34.0	41.0
17	38.7109457295161	40.0	38.0	41.0	35.0	41.0
18	38.726961538155074	40.0	38.0	41.0	35.0	41.0
19	38.77392849459367	40.0	38.0	41.0	35.0	41.0
20	38.75902662130182	40.0	38.0	41.0	35.0	41.0
21	38.68474195424738	40.0	38.0	41.0	34.0	41.0
22	38.61970024143234	40.0	38.0	41.0	34.0	41.0
23	38.571812176795405	40.0	38.0	41.0	34.0	41.0
24	38.51044374148413	40.0	38.0	41.0	34.0	41.0
25	38.452249782870254	40.0	38.0	41.0	34.0	41.0
26	38.20844933506506	40.0	37.0	41.0	34.0	41.0
27	38.06904327455558	40.0	37.0	41.0	33.0	41.0
28	37.95720671548434	40.0	37.0	41.0	33.0	41.0
29	37.81285726806958	40.0	37.0	41.0	33.0	41.0
30	37.68408538577382	40.0	37.0	41.0	33.0	41.0
31	37.56581063099099	40.0	36.0	41.0	33.0	41.0
32	37.40031394172158	40.0	36.0	41.0	32.0	41.0
33	37.31506999944224	40.0	35.0	41.0	32.0	41.0
34	37.1735109680401	39.0	35.0	41.0	32.0	41.0
35	37.067535716846876	39.0	35.0	41.0	31.0	41.0
36	36.94662831371861	39.0	35.0	41.0	31.0	41.0
37	36.679478251169314	39.0	35.0	41.0	31.0	41.0
38	36.49983346746241	39.0	35.0	41.0	30.0	41.0
39	36.33222046039474	39.0	35.0	41.0	30.0	41.0
40	36.13352881650346	39.0	35.0	41.0	29.0	41.0
41	35.89549884064669	39.0	35.0	41.0	28.0	41.0
42	35.703186428793394	39.0	35.0	41.0	27.0	41.0
43	34.83790886128397	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	1.0
13	3.0
14	2.0
15	2.0
16	2.0
17	3.0
18	23.0
19	37.0
20	106.0
21	218.0
22	433.0
23	732.0
24	1235.0
25	1852.0
26	2942.0
27	4373.0
28	6121.0
29	8413.0
30	11005.0
31	14077.0
32	17642.0
33	22748.0
34	29387.0
35	37744.0
36	52721.0
37	82772.0
38	133223.0
39	199682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.13669213791125	19.176580266292696	12.423327304164907	23.263400291631143
2	18.42359821834089	21.77130062708664	34.49613947299225	25.30896168158023
3	21.523334475422505	24.476936438753476	29.014430163903075	24.98529892192094
4	14.77310937761452	15.762264842511215	32.76324491438316	36.70138086549111
5	13.916064413829371	38.501366522975914	32.934080206532215	14.648488856662498
6	34.30426849188453	33.06762495916367	16.200667723763157	16.427438825188645
7	28.097784081401745	31.537597309981596	21.529390204062118	18.835228404554545
8	28.56375646409192	32.578226468314995	19.87410458880806	18.98391247878503
9	27.326634847531096	13.23702599979283	20.805730631628432	38.63060852104764
10	18.1499749005984	27.732209305105137	31.57138190133943	22.54643389295703
11	33.70953219496259	22.647309583190573	21.09736177400977	22.54579644783707
12	21.360945331112898	27.15691508434196	29.063194715579954	22.418944868965188
13	30.033067465597885	20.534179010525815	26.177799380084622	23.254954143791686
14	22.87137154285623	21.594250245017967	27.070541270587483	28.46383694153831
15	26.252061736559867	26.854447374921314	24.562513446108	22.33097744241082
16	23.822280300555374	26.297479701356963	26.481382618465194	23.398857379622473
17	22.166516601461343	27.422889060644934	27.34814862032972	23.062445717564003
18	21.787077393805625	25.28250770910192	29.34191759428212	23.588497302810335
19	22.57734998127505	26.12058868056828	29.85872622528904	21.443335112867626
20	23.04794384108493	24.97956191584131	30.177926869108614	21.794567373965148
21	23.79327654759723	25.282188986541943	28.974749205185617	21.949785260675213
22	23.72379502952168	25.487286953888816	28.45172548425909	22.33719253233042
23	22.84316459629804	25.991346682496552	28.73411367240102	22.43137504880439
24	22.490179361120628	26.625445215575972	28.614592712408665	22.269782710894734
25	22.588505270874336	26.170946845045055	28.83403319495462	22.406514689125984
26	22.45033904112318	26.453653755746963	28.788455868877538	22.307551334252317
27	22.51392419183911	26.510545732703328	28.492203249376498	22.48332682608107
28	21.87137951092023	26.543374156381223	29.402315519398254	22.182930813300292
29	21.89592114803866	26.992932327232456	29.322634879403353	21.788511645325535
30	21.748671325328083	27.3702998382483	29.062238547900016	21.818790288523598
31	21.912175998597622	27.345598839849885	28.613955267288706	22.12826989426379
32	21.260707085999314	27.273089457454525	29.12901092421574	22.33719253233042
33	21.39138333559095	27.077712528187025	29.36167839300085	22.16922574322117
34	21.839825977482253	26.864009051720707	28.918972757189188	22.37719221360786
35	21.684289368212205	27.12838941522378	28.863036947912768	22.324284268651244
36	21.349152596393655	27.085043147066557	28.543198858973234	23.022605397566554
37	21.457518266786717	26.68552441813213	28.98064557254524	22.876311742535915
38	21.207161695922743	26.341622775914136	29.505900351391624	22.9453151767715
39	21.20795850232269	26.463534155106334	29.64900678082246	22.67950056174851
40	20.842383726026085	26.439789324387853	29.9390443104039	22.778782639182158
41	20.371789866216204	26.472458386785764	29.97378506944168	23.181966677556353
42	20.36605286013657	26.48345431510506	30.010916247679305	23.13957657707907
43	20.082867865594697	26.35867443287305	29.76231265089521	23.796145050637048
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	249.0
1	229.0
2	209.0
3	565.5
4	922.0
5	922.0
6	1356.5
7	1791.0
8	1846.5
9	1902.0
10	2832.0
11	3762.0
12	3762.0
13	6405.0
14	9048.0
15	12077.5
16	15107.0
17	13771.0
18	12435.0
19	12435.0
20	13525.5
21	14616.0
22	11816.5
23	9017.0
24	9221.0
25	9425.0
26	9425.0
27	10592.0
28	11759.0
29	13020.0
30	14281.0
31	15732.0
32	17183.0
33	17183.0
34	19260.0
35	21337.0
36	23308.5
37	25280.0
38	28304.5
39	31329.0
40	31329.0
41	32946.5
42	34564.0
43	36118.5
44	37673.0
45	38661.5
46	39650.0
47	39650.0
48	40971.5
49	42293.0
50	41628.5
51	40964.0
52	40238.5
53	39513.0
54	39513.0
55	37664.0
56	35815.0
57	34412.0
58	33009.0
59	31354.5
60	29700.0
61	29700.0
62	27843.0
63	25986.0
64	23437.5
65	20889.0
66	18428.5
67	15968.0
68	15968.0
69	13684.0
70	11400.0
71	9743.5
72	8087.0
73	6668.5
74	5250.0
75	5250.0
76	4156.5
77	3063.0
78	2481.5
79	1900.0
80	1491.0
81	1082.0
82	1082.0
83	796.5
84	511.0
85	394.5
86	278.0
87	224.0
88	170.0
89	170.0
90	121.5
91	73.0
92	42.5
93	12.0
94	7.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	627505.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.59117115888327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.9559090465196	61.20954720891515
2	7.597683960741983	10.574634498461949
3	1.8518446939644466	3.8661612317204876
4	0.7652460226387504	2.130174677604318
5	0.4138910252847495	1.4401580590858345
6	0.27510021281410213	1.1486727597474835
7	0.1813600204514123	0.8834739357228961
8	0.13361084455218505	0.7438508121531245
9	0.09857072874841219	0.6173687210027947
>10	0.6494432374542861	8.514422804156107
>50	0.052856813585492504	2.49555610069759
>100	0.0224240424109548	2.71020118945614
>500	0.0011440837964544193	0.6278685834252109
>1k	4.576335185817677E-4	1.2162465737260364
>5k	4.576335185817677E-4	1.8216628441248828
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5821	0.9276420108206309	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5619	0.8954510322626912	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4403	0.7016677157950932	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3235	0.5155337407670059	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	957	0.15250874495023944	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	916	0.1459749324706576	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	820	0.1306762495916367	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	697	0.11107481215289121	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.1872255997960177E-4	0.0
3	0.0	0.0	0.0	7.968063999490044E-4	0.0
4	0.0	0.0	0.0	0.001274890239918407	0.0
5	0.0	0.0	0.0	0.001434251519908208	0.0
6	0.0	0.0	0.0	0.0015936127998980088	0.0
7	0.0	0.0	0.0	0.0015936127998980088	0.0
8	0.0	0.0	0.0	0.0015936127998980088	0.0
9	0.0	0.0	0.0	0.002549780479836814	0.0
10	0.0	0.0	0.0	0.0035059481597756194	0.0
11	0.0	0.0	0.0	0.004780838399694026	0.0
12	0.0	0.0	0.0	0.006693173759571637	0.0
13	0.0	0.0	0.0	0.008127425279479844	0.0
14	0.0	0.0	0.0	0.01099592831929626	0.0
15	0.0	0.0	0.0	0.01689229567891889	0.0
16	0.0	0.0	0.0	0.02486035967840894	0.0
17	0.0	0.0	0.0	0.0363343718376746	0.0
18	0.0	0.0	0.0	0.040796487677389026	0.0
19	0.0	0.0	0.0	0.05131433215671588	0.0
20	0.0	0.0	0.0	0.05768878335630792	0.0
21	0.0	0.0	0.0	0.06884407295559399	0.0
22	0.0	0.0	0.0	0.09402315519398252	0.0
23	0.0	0.0	0.0	0.1292419980717285	0.0
24	0.0	0.0	0.0	0.19330523262762847	0.0
25	0.0	0.0	0.0	0.20892263806662895	0.0
26	0.0	0.0	0.0	0.2345798041449869	0.0
27	0.0	0.0	0.0	0.2715516211026207	0.0
28	0.0	0.0	0.0	0.33179018493876544	0.0
29	0.0	1.5936127998980088E-4	0.0	0.4138612441335129	0.0
30	0.0	1.5936127998980088E-4	0.0	0.5467685516450068	0.0
31	0.0	1.5936127998980088E-4	0.0	0.9246141465008247	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACCGA	25	0.005494465	29.6	25
GTATTGA	45	0.003823902	20.555555	1
CGGGGTA	45	0.003823902	20.555555	30
TAGAACT	135	5.638867E-11	19.185186	4
TAGGCCG	50	0.007031409	18.5	5
TCACGTA	120	5.160473E-9	18.5	25
ATCACGT	125	8.556526E-9	17.76	24
TCTTGTA	105	4.7886897E-7	17.619047	2
CCTAGAA	85	2.7199263E-5	17.411764	2
GCCGCAC	75	2.0653814E-4	17.266666	12
CTAGGAC	75	2.0653814E-4	17.266666	3
GCCTACA	65	0.0015788535	17.076923	1
AGTCGGT	175	7.2759576E-12	16.914286	11
ATAGGCC	80	3.3795042E-4	16.1875	3
GTTTAAG	70	0.0025907347	15.857143	1
TTAGAAC	70	0.0025907347	15.857143	3
ATAAGAC	70	0.0025907347	15.857143	3
TAGAGGT	105	9.329502E-6	15.857142	4
CAGTCGG	190	2.7284841E-11	15.578948	10
TTAATCG	145	5.331276E-8	15.310345	37
>>END_MODULE