FastQCFastQC Report
Fri 10 Feb 2017
ERR1630557.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630557.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414058
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT8410.20311164136425333No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT7100.17147356167493444RNA PCR Primer, Index 46 (95% over 21bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5370.12969197552033773No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC4570.11037101082457046No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC4550.10988798670717628No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC4150.10022750435929265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT1400.027.75000236
CCTATAC604.308822E-827.7499983
CCCTATA556.2455E-726.909092
CTATACC358.859773E-426.4285724
ACTACAC451.3212155E-424.6666663
TAGGACG400.001929112123.1250024
TATACTC400.001929112123.1250025
GGTATCA2050.022.5609761
ATGCCGT1700.021.76470634
CCGTCTT1950.020.87179637
CTTAGGA806.9380076E-720.81252
TTTACTC450.00382173720.5555553
TACACAT2250.020.5555555
CGGACCG450.00382173720.5555555
TACCCCG555.1354605E-420.1818185
TATTCTG555.1354605E-420.1818185
CTACACA555.1354605E-420.1818184
ATTGCCG1202.3646862E-1020.04166611
TGCCGTC1850.020.035
TATACAC2150.019.7906993
Produced by FastQC (version 0.11.5)