FastQCFastQC Report
Fri 10 Feb 2017
ERR1630556.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630556.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805951
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT87670.48545060192663037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80700.44685597781999625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59680.3304630081325573No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37160.20576416525143817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2600.017.07692331
TATGCCG2600.017.07692333
TCTAGCG1901.8189894E-1216.55263128
CTCGTAT2750.016.14545429
AGTCGGT5300.016.05660411
CAGTCGG5550.015.66666510
GCCTAAG2250.015.6222221
GCCGTCT2850.015.57894736
GCAGTCG5650.015.389389
TCGTATG2900.015.31034530
CTTATAC11600.014.83189737
CGGTGAT5500.014.814
CTAGCGG2151.2732926E-1114.62790829
TGCCGTC3050.014.55737735
ACGAACG1405.999809E-714.53571415
CGCCTTA4350.014.45976925
TCTTATA18200.014.332417537
ATGCCGT3100.014.32258134
TCTCGTA3250.014.23076928
TCGGTGA6000.014.18333213
Produced by FastQC (version 0.11.5)