FastQCFastQC Report
Fri 10 Feb 2017
ERR1630555.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630555.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788374
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT83310.46584215605907936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76810.429496291044267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62450.34919988771923544No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG43120.24111287683672428No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT40890.22864344930087332No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40130.2243937789299106No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG38790.21690093906531854No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG36780.20566167926843043No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA34530.19308041830176462No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC31650.1769764042644324No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC26800.14985679729184162No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT26410.14767604539095291No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA25100.14035095567258302No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC24750.1383938706333239No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT23740.13274628237717614No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA23490.13134836449199105No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG23440.13106878091495405No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG23410.1309010307687318No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC22910.12810519499836165No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA22400.12525344251258405No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC21900.12245760674221388No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG21720.12145110586488063No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC21660.12111560557243618No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA20710.11580351760873285No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA20250.11323134869999228No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG19440.10870209475199259No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC19090.10674500971273346No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG18710.10462017452725213No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG18540.10366959036532628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA250.00549715129.5999982
AGTCGGT2750.020.18181811
CGGTGAT3000.017.88333314
ATACCGT2350.017.3191496
CGCCTTA2400.016.95833425
GCAGTCG3200.016.7656259
CGAACGA1452.9831426E-916.58620616
CGTCGTA2150.016.34883710
TACCGTC2500.016.2799997
CAGTCGG3300.016.25757610
ACGAACG1504.6748028E-916.03333315
GCGTTAT1751.3278623E-1015.8571431
TCGGTGA3500.015.85714313
GGTATCA41450.015.7997581
CGAGCCG3100.015.516129515
GTCGGTG3350.015.46268712
ACCGTCG2300.015.2826098
AGCGTCA855.366222E-415.2352943
CAAATCG2201.8189894E-1215.13636413
CCGTCGT2450.015.102049
Produced by FastQC (version 0.11.5)