##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630555.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1788374
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03252507585103	33.0	31.0	34.0	30.0	34.0
2	32.19824880030687	33.0	31.0	34.0	30.0	34.0
3	32.31081865426359	34.0	31.0	34.0	30.0	34.0
4	35.89824947130746	37.0	35.0	37.0	35.0	37.0
5	35.8592073022757	37.0	35.0	37.0	35.0	37.0
6	35.934879393236535	37.0	35.0	37.0	35.0	37.0
7	35.86311364401406	37.0	35.0	37.0	35.0	37.0
8	35.868091350019625	37.0	35.0	37.0	35.0	37.0
9	37.59697076785952	39.0	37.0	39.0	35.0	39.0
10	37.49817655591056	39.0	37.0	39.0	35.0	39.0
11	37.60311265987987	39.0	37.0	39.0	35.0	39.0
12	37.51254715177027	39.0	37.0	39.0	35.0	39.0
13	37.57273199006472	39.0	37.0	39.0	35.0	39.0
14	38.86792192237194	40.0	38.0	41.0	35.0	41.0
15	38.8741476894654	40.0	38.0	41.0	35.0	41.0
16	38.84694085241678	40.0	38.0	41.0	35.0	41.0
17	38.80219406007915	40.0	38.0	41.0	35.0	41.0
18	38.79675280450286	40.0	38.0	41.0	35.0	41.0
19	38.851570197285355	40.0	38.0	41.0	35.0	41.0
20	38.8265139171113	40.0	38.0	41.0	35.0	41.0
21	38.78577803076985	40.0	38.0	41.0	35.0	41.0
22	38.71674269476072	40.0	38.0	41.0	35.0	41.0
23	38.66521208650987	40.0	38.0	41.0	34.0	41.0
24	38.61559215242449	40.0	38.0	41.0	34.0	41.0
25	38.570543968990826	40.0	38.0	41.0	34.0	41.0
26	38.435232227710756	40.0	38.0	41.0	34.0	41.0
27	38.29915274992815	40.0	38.0	41.0	34.0	41.0
28	38.19403323913231	40.0	38.0	41.0	33.0	41.0
29	38.10724155014555	40.0	38.0	41.0	33.0	41.0
30	38.02291299247249	40.0	37.0	41.0	33.0	41.0
31	37.94053257316423	40.0	37.0	41.0	33.0	41.0
32	37.842947839769536	40.0	37.0	41.0	33.0	41.0
33	37.75321213571658	40.0	37.0	41.0	33.0	41.0
34	37.65578956079657	40.0	37.0	41.0	33.0	41.0
35	37.60196692638117	40.0	37.0	41.0	33.0	41.0
36	37.53891411975347	40.0	37.0	41.0	33.0	41.0
37	37.38244125669463	40.0	36.0	41.0	32.0	41.0
38	37.25467379865733	40.0	36.0	41.0	32.0	41.0
39	37.15652430643702	39.0	36.0	41.0	31.0	41.0
40	36.96010398272397	39.0	35.0	41.0	31.0	41.0
41	36.870763050681795	39.0	35.0	41.0	31.0	41.0
42	36.724201984596064	39.0	35.0	41.0	31.0	41.0
43	35.90627184246696	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	4.0
11	7.0
12	5.0
13	5.0
14	6.0
15	5.0
16	14.0
17	27.0
18	45.0
19	89.0
20	170.0
21	404.0
22	797.0
23	1408.0
24	2381.0
25	3968.0
26	6287.0
27	9471.0
28	13567.0
29	19185.0
30	26056.0
31	33803.0
32	43505.0
33	55686.0
34	73424.0
35	99926.0
36	142030.0
37	226540.0
38	395961.0
39	633593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.07884033205582	18.38368260777667	11.180994579433609	24.356482480733895
2	19.19408356417617	20.699249709512664	34.283153300148626	25.823513426162535
3	21.04218692510627	22.39291110248751	28.34770579308355	28.217196179322666
4	13.55253431329241	14.959958040096758	33.7640784310217	37.723429215589135
5	15.13111910595882	36.751261201516016	33.098278100665745	15.019341591859419
6	36.68863448025972	33.39838311225728	15.570736322491827	14.342246084991169
7	31.000115188433742	29.68769396110657	20.136447968937148	19.175742881522545
8	28.697297097810637	32.272947381252465	19.557374464178075	19.472381056758824
9	27.090082946855638	12.376382121413082	19.380957227067714	41.15257770466357
10	16.948747856991883	26.55976881793182	32.52295101583897	23.968532309237332
11	36.787942566823276	21.369914794109064	20.24749856573625	21.594644073331416
12	22.280127087510778	24.996393371856225	28.417378020481177	24.306101520151824
13	31.141640395129876	19.16232286982477	25.089382869578735	24.60665386546662
14	23.59467315002343	20.4298988913952	25.007408964791484	30.96801899378989
15	25.786105143554984	27.32057164776495	22.92294564783429	23.970377560845773
16	26.900637115055353	25.20199913440925	23.613237499538688	24.284126250996714
17	24.125210945808874	25.730691678586247	24.767638089124535	25.376459286480348
18	23.500900818285213	23.71590058902668	27.543232008517233	25.23996658417087
19	25.747019359485208	24.984147611182	26.172769230597183	23.09606379873561
20	25.889383316912458	24.047263044530954	26.302943344065614	23.760410294490974
21	25.422981993699306	24.538491389384994	25.61762808003248	24.420898536883225
22	26.21090443050503	23.520919002401065	25.84929103196535	24.41888553512856
23	23.487984057026104	24.542741059755958	26.46280923341538	25.506465649802557
24	24.412734696433745	25.325630992175014	25.681932302750994	24.57970200864025
25	24.521660458047364	24.88299427301001	26.070273891255408	24.52507137768722
26	25.421136742090862	25.03139723570126	25.056224257342148	24.491241764865737
27	25.578933712970553	24.33953971596545	24.903347957418305	25.17817861364569
28	24.238330461078053	25.012832886185997	26.327267115267833	24.421569537468113
29	24.451205396634037	25.564339450249218	26.525380037956264	23.459075115160474
30	24.529153297911957	25.05482633945696	25.593527975691888	24.822492386939196
31	23.642873358704612	24.836583399221862	26.114951346865922	25.405591895207603
32	23.136938917698423	25.43192866816449	25.90615833153468	25.52497408260241
33	23.89813316453941	24.536813887922772	26.397834010111982	25.16721893742584
34	24.99359753608585	24.81125312714231	26.388495918638945	23.806653418132896
35	25.087649451401106	24.70529095144528	25.52939150311959	24.677668094034022
36	23.402207815591147	25.18533595321784	25.36605877741457	26.04639745377645
37	23.743914863445788	24.79352752835816	27.1827369442857	24.279820663910346
38	23.370614871385964	23.93515003013911	26.424338533215085	26.269896565259838
39	23.276730706216934	24.751254491510167	27.448341342470872	24.52367345980203
40	24.22026936200146	24.057216219873474	26.844999983224987	24.877514434900082
41	22.31887737128811	24.964297177212373	27.854073029466992	24.86275242203253
42	22.74691982773178	25.354707684186867	27.364354435928952	24.5340180521524
43	23.015935145556803	23.7878094850406	27.020187052596377	26.176068316806216
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	107.0
1	143.5
2	180.0
3	663.0
4	1146.0
5	1146.0
6	1783.5
7	2421.0
8	2438.0
9	2455.0
10	3408.0
11	4361.0
12	4361.0
13	7319.5
14	10278.0
15	14606.5
16	18935.0
17	17663.0
18	16391.0
19	16391.0
20	18262.5
21	20134.0
22	18192.0
23	16250.0
24	18433.0
25	20616.0
26	20616.0
27	23195.5
28	25775.0
29	29667.0
30	33559.0
31	38407.5
32	43256.0
33	43256.0
34	54418.0
35	65580.0
36	70882.0
37	76184.0
38	78483.0
39	80782.0
40	80782.0
41	83146.5
42	85511.0
43	91833.5
44	98156.0
45	98426.5
46	98697.0
47	98697.0
48	101219.5
49	103742.0
50	108287.0
51	112832.0
52	124490.5
53	136149.0
54	136149.0
55	135625.0
56	135101.0
57	123746.0
58	112391.0
59	112055.5
60	111720.0
61	111720.0
62	106317.5
63	100915.0
64	83528.5
65	66142.0
66	59714.0
67	53286.0
68	53286.0
69	48273.0
70	43260.0
71	43484.5
72	43709.0
73	37353.0
74	30997.0
75	30997.0
76	18889.0
77	6781.0
78	5427.5
79	4074.0
80	3351.0
81	2628.0
82	2628.0
83	2130.0
84	1632.0
85	1343.0
86	1054.0
87	900.0
88	746.0
89	746.0
90	562.5
91	379.0
92	209.0
93	39.0
94	28.5
95	18.0
96	18.0
97	11.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1788374.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.956971162952925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.20197043761489	44.26836321053925
2	9.565048811001036	9.939419304908359
3	2.3281852629867905	3.6289636371305
4	0.8976977295490809	1.8656662018891965
5	0.4537432921144143	1.1787563571885975
6	0.26316901729781855	0.820407902563525
7	0.17428821827356222	0.6338841551617342
8	0.12992924286146745	0.5400583939662057
9	0.10041192181070005	0.4695389393341704
>10	0.6612154890279781	6.865437695878319
>50	0.09992507717631724	3.6601654796098484
>100	0.09773990734370835	10.393886394822683
>500	0.016739744558987947	5.989635011524537
>1k	0.009611853327418885	8.496917561386832
>5k	3.239950559804119E-4	1.2488997540963025
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8331	0.46584215605907936	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7681	0.429496291044267	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6245	0.34919988771923544	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	4312	0.24111287683672428	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	4089	0.22864344930087332	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4013	0.2243937789299106	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	3879	0.21690093906531854	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	3678	0.20566167926843043	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	3453	0.19308041830176462	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	3165	0.1769764042644324	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	2680	0.14985679729184162	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	2641	0.14767604539095291	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	2510	0.14035095567258302	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	2475	0.1383938706333239	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	2374	0.13274628237717614	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	2349	0.13134836449199105	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	2344	0.13106878091495405	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	2341	0.1309010307687318	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	2291	0.12810519499836165	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	2240	0.12525344251258405	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	2190	0.12245760674221388	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	2172	0.12145110586488063	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	2166	0.12111560557243618	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	2071	0.11580351760873285	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	2025	0.11323134869999228	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	1944	0.10870209475199259	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1909	0.10674500971273346	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	1871	0.10462017452725213	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	1854	0.10366959036532628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.59167154074036E-5
2	0.0	0.0	0.0	3.9141700785182517E-4	5.59167154074036E-5
3	0.0	0.0	0.0	8.387507311110539E-4	5.59167154074036E-5
4	0.0	0.0	0.0	0.0015656680314073007	5.59167154074036E-5
5	0.0	0.0	0.0	0.0018452516084443186	5.59167154074036E-5
6	0.0	0.0	0.0	0.001957085039259126	1.118334308148072E-4
7	0.0	0.0	0.0	0.0022366686162961437	1.118334308148072E-4
8	0.0	0.0	0.0	0.002348502047110951	1.118334308148072E-4
9	0.0	0.0	0.0	0.0041937536555552695	1.118334308148072E-4
10	0.0	5.59167154074036E-5	0.0	0.0068777559951106425	1.118334308148072E-4
11	0.0	5.59167154074036E-5	0.0	0.0077724234416291	1.118334308148072E-4
12	0.0	5.59167154074036E-5	0.0	0.009282174757628997	1.118334308148072E-4
13	0.0	5.59167154074036E-5	0.0	0.010176842204147454	1.118334308148072E-4
14	0.0	5.59167154074036E-5	0.0	0.010680092642814087	1.118334308148072E-4
15	0.0	5.59167154074036E-5	0.0	0.01196617709718437	1.118334308148072E-4
16	0.0	1.118334308148072E-4	0.0	0.01509751315999897	1.118334308148072E-4
17	0.0	1.118334308148072E-4	0.0	0.019626767107998662	1.118334308148072E-4
18	0.0	1.118334308148072E-4	0.0	0.021975269155109615	1.118334308148072E-4
19	5.59167154074036E-5	1.118334308148072E-4	0.0	0.025777605802813058	1.118334308148072E-4
20	5.59167154074036E-5	1.118334308148072E-4	0.0	0.028964858581035063	1.118334308148072E-4
21	5.59167154074036E-5	1.118334308148072E-4	0.0	0.03505978056044205	1.118334308148072E-4
22	5.59167154074036E-5	1.118334308148072E-4	0.0	0.044845205756737685	1.118334308148072E-4
23	5.59167154074036E-5	1.118334308148072E-4	0.0	0.06201163738681059	1.118334308148072E-4
24	5.59167154074036E-5	1.118334308148072E-4	0.0	0.08790107662043846	1.118334308148072E-4
25	5.59167154074036E-5	1.118334308148072E-4	0.0	0.09399599859984545	1.118334308148072E-4
26	1.118334308148072E-4	1.118334308148072E-4	0.0	0.10484384138888174	1.118334308148072E-4
27	1.118334308148072E-4	1.118334308148072E-4	0.0	0.11686593520147352	1.118334308148072E-4
28	1.118334308148072E-4	1.118334308148072E-4	0.0	0.15047188116132307	1.118334308148072E-4
29	1.6775014622221078E-4	1.118334308148072E-4	0.0	0.19660317137243105	1.118334308148072E-4
30	1.6775014622221078E-4	1.118334308148072E-4	0.0	0.2630322292764265	1.118334308148072E-4
31	1.6775014622221078E-4	1.118334308148072E-4	0.0	0.5354584667412968	1.118334308148072E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	25	0.005497151	29.599998	2
AGTCGGT	275	0.0	20.181818	11
CGGTGAT	300	0.0	17.883333	14
ATACCGT	235	0.0	17.319149	6
CGCCTTA	240	0.0	16.958334	25
GCAGTCG	320	0.0	16.765625	9
CGAACGA	145	2.9831426E-9	16.586206	16
CGTCGTA	215	0.0	16.348837	10
TACCGTC	250	0.0	16.279999	7
CAGTCGG	330	0.0	16.257576	10
ACGAACG	150	4.6748028E-9	16.033333	15
GCGTTAT	175	1.3278623E-10	15.857143	1
TCGGTGA	350	0.0	15.857143	13
GGTATCA	4145	0.0	15.799758	1
CGAGCCG	310	0.0	15.5161295	15
GTCGGTG	335	0.0	15.462687	12
ACCGTCG	230	0.0	15.282609	8
AGCGTCA	85	5.366222E-4	15.235294	3
CAAATCG	220	1.8189894E-12	15.136364	13
CCGTCGT	245	0.0	15.10204	9
>>END_MODULE