FastQCFastQC Report
Fri 10 Feb 2017
ERR1630554.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630554.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742383
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60840.3491769605190133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55860.320595414441027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45230.2595870138769719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29070.1668404707805345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA652.6841662E-622.7692315
TCTTATA17100.016.55263137
TATAGTG1352.2213499E-816.4444455
GTATTAT1951.8189894E-1216.1282061
TGCGCTA1151.2430191E-616.0869565
GGTATCA28250.016.0442491
CGTTATT1851.8189894E-1116.02
CTATACT2100.015.8571444
TCTATAC1403.4760888E-815.8571423
TATACTT2400.015.4166665
GTATTAG2300.015.2826091
GTCGCCA3900.015.17948712
TCGCCAT4350.014.885056513
AATCGAG2500.014.80000122
CGGTTTA750.004105535314.815
GCCCTAG1508.1079634E-814.81
TAATACT2450.014.3469384
GCGTTAT1956.730261E-1014.230771
CGAACGA1304.4468416E-614.23076916
TAGTGCG1702.4387191E-814.1470592
Produced by FastQC (version 0.11.5)