FastQCFastQC Report
Fri 10 Feb 2017
ERR1630553.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630553.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358538
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72970.5371215232845897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64250.47293487557948327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51990.3826908043794138No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31440.23142525273492534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTG450.003826160220.5555575
TACGTTA701.2194043E-418.519
ACGAACG1002.8758768E-718.515
GCGTTAT1156.40739E-817.6956521
TGCGTAC852.723705E-517.41176616
ACGTTAA752.067615E-417.26666620
TTACACT2700.017.129634
TATACTG1107.8101766E-716.8181825
ACGCTAT904.4469132E-516.44444510
TATACGC904.4469132E-516.4444457
GTATGGT2150.016.3488373
ATAGATC1251.6582453E-716.2799993
ATAGTAC803.3831334E-416.18753
TAATACT1951.8189894E-1216.1282064
CGAACGA1151.2425262E-616.08695616
CTAGCGG1403.474088E-815.85714329
CGGTGAT2600.015.65384614
TACGCTA957.061185E-515.5789489
GTGCGTA957.061185E-515.57894815
CAGTCGG2650.015.35849110
Produced by FastQC (version 0.11.5)