##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630553.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1358538
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.566045999449408	31.0	31.0	34.0	30.0	34.0
2	31.75481289444977	31.0	31.0	34.0	30.0	34.0
3	31.838468265149743	33.0	31.0	34.0	30.0	34.0
4	35.558166941226524	37.0	35.0	37.0	33.0	37.0
5	35.42017595385628	37.0	35.0	37.0	33.0	37.0
6	35.49946118548027	37.0	35.0	37.0	33.0	37.0
7	35.45619482119749	37.0	35.0	37.0	33.0	37.0
8	35.4528191335097	37.0	35.0	37.0	33.0	37.0
9	37.08306429411618	39.0	37.0	39.0	33.0	39.0
10	36.91405613976201	39.0	37.0	39.0	33.0	39.0
11	37.04627548143666	39.0	37.0	39.0	33.0	39.0
12	36.972067766967136	39.0	37.0	39.0	33.0	39.0
13	37.033940162144894	39.0	37.0	39.0	33.0	39.0
14	38.18797339492896	40.0	38.0	41.0	33.0	41.0
15	38.18154221670649	40.0	38.0	41.0	33.0	41.0
16	38.123743318184694	40.0	37.0	41.0	33.0	41.0
17	38.10325806123936	40.0	37.0	41.0	33.0	41.0
18	38.0863413463591	40.0	37.0	41.0	33.0	41.0
19	38.162688861113935	40.0	38.0	41.0	34.0	41.0
20	38.12571013839878	40.0	38.0	41.0	33.0	41.0
21	38.07511751603562	40.0	37.0	41.0	33.0	41.0
22	38.00748304427259	40.0	37.0	41.0	33.0	41.0
23	37.9373848946441	40.0	37.0	41.0	33.0	41.0
24	37.867810101741725	40.0	37.0	41.0	33.0	41.0
25	37.81060448805996	40.0	37.0	41.0	33.0	41.0
26	37.645725036767466	39.0	37.0	41.0	33.0	41.0
27	37.500761848398795	39.0	37.0	41.0	32.0	41.0
28	37.394493197834734	39.0	36.0	40.0	32.0	41.0
29	37.30229702812877	39.0	36.0	40.0	32.0	41.0
30	37.19881372475411	39.0	36.0	40.0	31.0	41.0
31	37.13272282409473	39.0	36.0	40.0	31.0	41.0
32	37.00556701395176	39.0	36.0	40.0	31.0	41.0
33	36.926999465601995	39.0	36.0	40.0	31.0	41.0
34	36.84390057547157	39.0	35.0	40.0	31.0	41.0
35	36.77826899210769	39.0	35.0	40.0	31.0	41.0
36	36.74258946014024	39.0	35.0	40.0	30.0	41.0
37	36.59655305924457	39.0	35.0	40.0	30.0	41.0
38	36.421787244817594	39.0	35.0	40.0	30.0	41.0
39	36.35484542942487	39.0	35.0	40.0	30.0	41.0
40	36.15912252730509	38.0	35.0	40.0	30.0	41.0
41	36.087877556608646	38.0	35.0	40.0	29.0	41.0
42	35.940060565107494	38.0	35.0	40.0	29.0	41.0
43	35.10664037369584	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	3.0
12	4.0
13	4.0
14	5.0
15	6.0
16	14.0
17	45.0
18	83.0
19	142.0
20	329.0
21	624.0
22	1018.0
23	1807.0
24	2863.0
25	4627.0
26	6745.0
27	9927.0
28	14210.0
29	19592.0
30	26043.0
31	33411.0
32	43114.0
33	54856.0
34	72029.0
35	96460.0
36	141441.0
37	225783.0
38	371604.0
39	231740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27843608349564	19.817480261869747	12.69688444489591	24.207199209738704
2	18.59719787006326	22.101038027644424	34.99997791743771	24.301786184854603
3	20.530599806556754	24.18121539478469	30.194002670517868	25.09418212814069
4	14.821963022013371	16.079344118456753	34.23651013074349	34.86218272878639
5	13.762220858010595	38.25906967637269	34.17379565385731	13.804913811759405
6	34.21111518411704	34.996150273308515	16.06962779105185	14.723106751522593
7	28.212755182409328	31.493635069464382	21.67940830510446	18.614201443021834
8	27.16810276930053	33.9322124224718	20.323612589415976	18.576072218811692
9	26.616406754908585	13.692660786816417	20.286219450615295	39.4047130076597
10	16.481393969105024	27.889245644950677	33.26833699167781	22.361023394266482
11	34.4771364510967	22.232355664692484	21.951465472441697	21.33904241176912
12	21.067132461513776	26.57187358763612	29.555816620514115	22.805177330335994
13	29.70641969529008	20.13878154310001	26.312550697882575	23.842248063727332
14	22.811360447775474	21.536754952750677	26.257491509254805	29.394393090219044
15	25.468334341770344	28.088577573833046	23.426359807381168	23.01672827701544
16	24.58164585753214	26.930788833289903	24.894408548012642	23.59315676116531
17	22.802232988698144	27.363091794267074	26.374823523523084	23.459851693511702
18	22.309939066849804	25.5579159361019	28.36137082657975	23.77077417046855
19	23.436738611654587	26.063238569697717	28.358720919105686	22.141301899542007
20	24.06138069012424	25.062898498238546	28.219600776717325	22.65612003491989
21	24.153538583388908	25.861624776046018	26.965090413370845	23.019746227194236
22	23.914678868018413	25.587948220807956	27.21322480490056	23.28414810627307
23	23.142304447869698	26.009209900643192	27.50736453452167	23.341121116965443
24	23.73058390711191	26.215902683620186	27.329231865431808	22.724281543836096
25	23.641443964026035	25.66501636317865	27.49853150960812	23.195008163187193
26	23.451681145466672	26.35811438472829	27.255770541567482	22.93443392823756
27	23.667133344816264	25.82408442016344	26.94690910375713	23.561873131263166
28	22.911836106167073	26.169308477201227	27.72539303280438	23.19346238382732
29	22.60878974309147	26.605439082307598	27.87437671967954	22.911394454921393
30	22.525980134526968	27.23184776575996	27.676516961616088	22.565655138096986
31	23.265745971036512	26.69347489727928	27.223898043337762	22.816881088346445
32	22.27961234797996	26.651518028939936	27.572360876177182	23.49650874690292
33	22.459879664757263	26.308134185425804	27.91051851328413	23.3214676365328
34	23.085405045718264	26.193525687172535	27.572360876177182	23.148708390932015
35	22.98029204924706	26.2633801925305	28.02262432114523	22.73370343707721
36	22.62358505982166	26.63208537413013	27.481601545190493	23.26272802085772
37	23.23122356533273	25.70366084717542	27.563969502509316	23.501146084982533
38	22.575960333829457	25.925958640833013	28.302410385281824	23.195670640055706
39	22.679601159481738	25.72957105358849	28.255595353240025	23.335232433689747
40	22.062467152188603	25.67686733827099	28.951343282263725	23.309322227276674
41	21.52328458975752	25.95893526717692	29.188951652438135	23.328828490627423
42	22.32385108108864	25.44095196453835	28.865883766225163	23.369313188147846
43	21.452252347744412	25.363000519676298	29.07780275560934	24.10694437696995
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	143.0
2	168.0
3	500.5
4	833.0
5	833.0
6	1361.5
7	1890.0
8	1965.0
9	2040.0
10	2861.5
11	3683.0
12	3683.0
13	6145.0
14	8607.0
15	12979.0
16	17351.0
17	16406.0
18	15461.0
19	15461.0
20	17409.5
21	19358.0
22	18545.5
23	17733.0
24	19741.0
25	21749.0
26	21749.0
27	24264.5
28	26780.0
29	32517.0
30	38254.0
31	41989.5
32	45725.0
33	45725.0
34	49615.0
35	53505.0
36	57316.5
37	61128.0
38	67569.5
39	74011.0
40	74011.0
41	78683.5
42	83356.0
43	81285.5
44	79215.0
45	82766.5
46	86318.0
47	86318.0
48	88701.5
49	91085.0
50	93852.5
51	96620.0
52	99933.0
53	103246.0
54	103246.0
55	94932.5
56	86619.0
57	81999.5
58	77380.0
59	70214.5
60	63049.0
61	63049.0
62	58333.0
63	53617.0
64	47580.0
65	41543.0
66	35690.5
67	29838.0
68	29838.0
69	25539.0
70	21240.0
71	18287.0
72	15334.0
73	12203.5
74	9073.0
75	9073.0
76	7105.5
77	5138.0
78	4251.5
79	3365.0
80	2604.5
81	1844.0
82	1844.0
83	1382.5
84	921.0
85	767.0
86	613.0
87	535.0
88	457.0
89	457.0
90	344.5
91	232.0
92	128.0
93	24.0
94	18.0
95	12.0
96	12.0
97	7.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1358538.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.69329651138171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.52294973658904	52.245333901769676
2	7.817091924863181	9.332559722551709
3	1.8783599452496942	3.363764915006781
4	0.7737674601310212	1.8475492171383907
5	0.4138070955026374	1.235075482517629
6	0.2674768667783516	0.957994555112129
7	0.18280606037960248	0.7638607456422011
8	0.1334660681758116	0.6373623665461607
9	0.1033558063867919	0.5552683917137755
>10	0.6820642073961795	8.13569959406668
>50	0.11014501731273184	4.66314369431879
>100	0.1083989291543484	12.2693311798175
>500	0.005444682579432907	2.1237994900695427
>1k	4.949711435848098E-4	0.47163535559592484
>5k	3.7122835768860733E-4	1.3976213881332018
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7297	0.5371215232845897	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6425	0.47293487557948327	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5199	0.3826908043794138	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3144	0.23142525273492534	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.360854094622307E-5	0.0
2	0.0	0.0	0.0	6.624768685160077E-4	0.0
3	0.0	0.0	0.0	0.001177736655139569	0.0
4	0.0	0.0	0.0	0.002134647687440469	0.0
5	0.0	0.0	0.0	0.0025026903921715844	0.0
6	0.0	0.0	0.0	0.0025762989331178077	0.0
7	0.0	0.0	0.0	0.003091558719741369	0.0
8	0.0	0.0	0.0	0.0033123843425800384	0.0
9	7.360854094622307E-5	0.0	0.0	0.006035900357590292	0.0
10	7.360854094622307E-5	0.0	0.0	0.008538590749761876	0.0
11	7.360854094622307E-5	0.0	0.0	0.009642718863955223	0.0
12	7.360854094622307E-5	0.0	0.0	0.010157978650578783	0.0
13	7.360854094622307E-5	0.0	0.0	0.011041281141933462	0.0
14	7.360854094622307E-5	0.0	0.0	0.011556540928557022	0.0
15	7.360854094622307E-5	0.0	0.0	0.013543971534105045	0.0
16	7.360854094622307E-5	0.0	0.0	0.016709138794792636	0.0
17	7.360854094622307E-5	0.0	0.0	0.01987430605548023	0.0
18	7.360854094622307E-5	0.0	0.0	0.021640911038189582	0.0
19	7.360854094622307E-5	0.0	0.0	0.02340751602089894	0.0
20	7.360854094622307E-5	0.0	0.0	0.02517412100360829	0.0
21	7.360854094622307E-5	0.0	0.0	0.030253110328897683	0.0
22	7.360854094622307E-5	0.0	0.0	0.038644483996767114	0.0
23	7.360854094622307E-5	0.0	0.0	0.05196762990803349	0.0
24	7.360854094622307E-5	0.0	0.0	0.06963367973512703	0.0
25	7.360854094622307E-5	0.0	0.0	0.07552236301082488	0.0
26	7.360854094622307E-5	0.0	0.0	0.08457621354721032	0.0
27	7.360854094622307E-5	0.0	0.0	0.09583832031198244	0.0
28	7.360854094622307E-5	7.360854094622307E-5	0.0	0.12101244131559073	0.0
29	7.360854094622307E-5	7.360854094622307E-5	0.0	0.15928888260762672	0.0
30	7.360854094622307E-5	7.360854094622307E-5	0.0	0.2236963559355719	0.0
31	7.360854094622307E-5	7.360854094622307E-5	0.0	0.4690336229093334	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTG	45	0.0038261602	20.555557	5
TACGTTA	70	1.2194043E-4	18.5	19
ACGAACG	100	2.8758768E-7	18.5	15
GCGTTAT	115	6.40739E-8	17.695652	1
TGCGTAC	85	2.723705E-5	17.411766	16
ACGTTAA	75	2.067615E-4	17.266666	20
TTACACT	270	0.0	17.12963	4
TATACTG	110	7.8101766E-7	16.818182	5
ACGCTAT	90	4.4469132E-5	16.444445	10
TATACGC	90	4.4469132E-5	16.444445	7
GTATGGT	215	0.0	16.348837	3
ATAGATC	125	1.6582453E-7	16.279999	3
ATAGTAC	80	3.3831334E-4	16.1875	3
TAATACT	195	1.8189894E-12	16.128206	4
CGAACGA	115	1.2425262E-6	16.086956	16
CTAGCGG	140	3.474088E-8	15.857143	29
CGGTGAT	260	0.0	15.653846	14
TACGCTA	95	7.061185E-5	15.578948	9
GTGCGTA	95	7.061185E-5	15.578948	15
CAGTCGG	265	0.0	15.358491	10
>>END_MODULE