FastQCFastQC Report
Fri 10 Feb 2017
ERR1630552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907618
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103790.5440816767298274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT87840.46046954893484965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81370.4265529052462285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59880.3138993236591393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA1054.0017767E-1122.9047615
TATCCCG450.003826720420.5555555
AGTCGGT2850.018.17543811
CAGTCGG2900.017.22413810
AGACGTA700.002593334315.8571435
GCAGTCG3200.015.6093759
CGGTGAT3500.015.32857214
GGTATCA58100.014.8063691
TGCGTTA750.00410572514.88
TCGGTGA3750.014.30666713
CGGACCA2850.014.2807029
TCTAGCG2101.364242E-1014.09523828
AATGCGT1051.656535E-414.0952386
CGCAATA2101.364242E-1014.09523836
GTCGGTG3700.014.012
TCTATGG5300.013.9622642
AAGACGG3050.013.950825
CTTATAC12850.013.67704337
TAGGACA8000.013.643754
CAAGACG3400.013.60294154
Produced by FastQC (version 0.11.5)