##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630552.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1907618
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09055114808101	33.0	31.0	34.0	30.0	34.0
2	32.26561292669707	34.0	31.0	34.0	30.0	34.0
3	32.36310676456188	34.0	31.0	34.0	30.0	34.0
4	35.93124514446813	37.0	35.0	37.0	35.0	37.0
5	35.894423306972364	37.0	35.0	37.0	35.0	37.0
6	35.9726884523002	37.0	35.0	37.0	35.0	37.0
7	35.92404558984031	37.0	35.0	37.0	35.0	37.0
8	35.931590077258655	37.0	35.0	37.0	35.0	37.0
9	37.63804493352443	39.0	37.0	39.0	35.0	39.0
10	37.57056758743103	39.0	37.0	39.0	35.0	39.0
11	37.65628024059324	39.0	37.0	39.0	35.0	39.0
12	37.61382677244605	39.0	37.0	39.0	35.0	39.0
13	37.64668240706472	39.0	37.0	39.0	35.0	39.0
14	38.957064255002834	40.0	38.0	41.0	36.0	41.0
15	38.95805973732686	40.0	38.0	41.0	36.0	41.0
16	38.911906366998004	40.0	38.0	41.0	35.0	41.0
17	38.9160717711827	40.0	38.0	41.0	36.0	41.0
18	38.9023918834903	40.0	38.0	41.0	35.0	41.0
19	38.980858326981604	40.0	38.0	41.0	35.0	41.0
20	38.95122451140637	40.0	38.0	41.0	35.0	41.0
21	38.91158921754775	40.0	38.0	41.0	35.0	41.0
22	38.841559997861204	40.0	38.0	41.0	35.0	41.0
23	38.785211714295	40.0	38.0	41.0	35.0	41.0
24	38.73468587526433	40.0	38.0	41.0	35.0	41.0
25	38.69097901152117	40.0	38.0	41.0	34.0	41.0
26	38.546515602180314	40.0	38.0	41.0	34.0	41.0
27	38.4158227695482	40.0	38.0	41.0	34.0	41.0
28	38.32439513571376	40.0	38.0	41.0	34.0	41.0
29	38.25005215928975	40.0	38.0	41.0	34.0	41.0
30	38.15244089749625	40.0	38.0	41.0	34.0	41.0
31	38.09142710962048	40.0	37.0	41.0	33.0	41.0
32	37.97624524406878	40.0	37.0	41.0	33.0	41.0
33	37.88673308807109	40.0	37.0	41.0	33.0	41.0
34	37.81672955486895	40.0	37.0	41.0	33.0	41.0
35	37.73572853684543	40.0	37.0	41.0	33.0	41.0
36	37.68132141760038	40.0	37.0	41.0	33.0	41.0
37	37.53124000717125	40.0	37.0	41.0	33.0	41.0
38	37.35897386164316	40.0	36.0	41.0	32.0	41.0
39	37.28396723033647	40.0	36.0	41.0	32.0	41.0
40	37.09366078533543	40.0	36.0	41.0	31.0	41.0
41	37.0046151797687	40.0	36.0	41.0	31.0	41.0
42	36.848748543995704	40.0	36.0	41.0	31.0	41.0
43	36.08444248271929	39.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	6.0
11	9.0
12	4.0
13	6.0
14	4.0
15	3.0
16	12.0
17	26.0
18	47.0
19	133.0
20	251.0
21	463.0
22	870.0
23	1538.0
24	2545.0
25	4139.0
26	6504.0
27	9613.0
28	13888.0
29	19082.0
30	26320.0
31	33952.0
32	43956.0
33	56605.0
34	75697.0
35	100769.0
36	142145.0
37	226138.0
38	411199.0
39	731688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83933261271387	19.646858018743796	12.2997895805135	24.21401978802884
2	18.317136869121594	21.992715522709474	34.99594782603225	24.694199782136675
3	20.762437762696724	24.508523194895414	29.58212807805336	25.146910964354497
4	14.580067917161612	15.896526453409434	34.32249014215634	35.20091548727261
5	13.722401445153064	38.094576587136416	34.08952945505862	14.093492512651903
6	33.8525847418089	34.33653907648177	16.355580624632395	15.455295557076942
7	27.66020240949708	31.346160499638813	22.270601346810526	18.72303574405358
8	26.772603319951898	33.36679565825024	20.84720316121991	19.013397860577957
9	26.505778410562282	14.16724941786039	20.92169396598271	38.40527820559462
10	17.10677923986878	27.59955085347276	32.96645345137234	22.327216455286123
11	33.70963159290801	22.721005987571935	22.028309651093668	21.541052768426383
12	20.928194219178053	26.82596830182982	29.367986672384095	22.877850806608034
13	28.941853138311757	20.756042352294852	26.67038159631541	23.631722913077986
14	22.679226134372815	22.159415564332065	26.274023415589493	28.887334885705627
15	25.291646440744426	27.932059772973417	24.036730624265445	22.73956316201671
16	24.259364296206055	27.111507649854428	25.51055819351673	23.118569860422788
17	22.757124329923496	27.35552925166359	26.68883392796671	23.1985124904462
18	21.911986571735014	25.69707352310578	28.792504579009005	23.598435326150206
19	23.045861383149038	26.293838703555956	28.843091226859883	21.817208686435123
20	23.490185141888993	25.244414762284695	28.916271496704265	22.349128599122047
21	23.550574590929628	26.178616473528766	27.661250837431812	22.60955809810979
22	23.48882218557384	25.8595274315927	27.76404919643241	22.887601186401053
23	22.762576155184107	26.295778295235213	28.085182672841206	22.85646287673947
24	23.12984046072117	26.704612768384443	27.851959878759793	22.313586892134587
25	23.16753144497483	26.030211499367272	28.04219712751714	22.76005992814075
26	22.960257242278065	26.707338681014754	27.871041267171936	22.461362809535242
27	23.23651800308028	26.14842174900845	27.4857964225542	23.129263825357068
28	22.355786116507602	26.461744437303487	28.40898964048358	22.773479805705335
29	22.18898123209154	26.979353308681297	28.294396467217233	22.53726899200993
30	21.844205705754508	27.640334700133884	28.354681073464395	22.160778520647213
31	22.69500497479055	27.106160667387286	27.7648879387802	22.433946419041966
32	21.817418372022072	26.957021793671483	27.981178621715667	23.24438121259078
33	21.87288020976946	26.791841972554252	28.402541808684965	22.932736008991316
34	22.584657934659873	26.607161391850987	28.01976076971385	22.788419903775285
35	22.456644883828943	26.70267317670519	28.37753680244158	22.46314513702429
36	22.030721035343554	27.08524453008936	27.987049818150172	22.896984616416912
37	22.765511753401363	25.977161045869774	28.059915559614133	23.197411641114734
38	22.06662969210817	26.208758776652346	28.814416722844932	22.91019480839455
39	22.18394877800482	26.10029890680419	28.624703688054947	23.09104862713604
40	21.639342887307627	25.969874471723376	29.377212838209747	23.013569802759253
41	21.07764762127428	26.348199691971875	29.552614831690622	23.021537855063226
42	21.802950066522754	25.850668215544204	29.271793409372314	23.074588308560728
43	21.053638621568886	25.691674119241902	29.366099502101573	23.888587757087635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	394.0
1	398.5
2	403.0
3	1135.0
4	1867.0
5	1867.0
6	2683.5
7	3500.0
8	3457.5
9	3415.0
10	5154.5
11	6894.0
12	6894.0
13	11540.5
14	16187.0
15	21479.0
16	26771.0
17	26012.0
18	25253.0
19	25253.0
20	28165.5
21	31078.0
22	29383.0
23	27688.0
24	29856.0
25	32024.0
26	32024.0
27	35557.5
28	39091.0
29	47612.5
30	56134.0
31	60769.0
32	65404.0
33	65404.0
34	70762.5
35	76121.0
36	81272.5
37	86424.0
38	96435.0
39	106446.0
40	106446.0
41	112096.5
42	117747.0
43	114868.5
44	111990.0
45	116160.5
46	120331.0
47	120331.0
48	123752.5
49	127174.0
50	130017.0
51	132860.0
52	136627.5
53	140395.0
54	140395.0
55	128423.5
56	116452.0
57	108873.5
58	101295.0
59	92086.5
60	82878.0
61	82878.0
62	76871.0
63	70864.0
64	63138.0
65	55412.0
66	48118.5
67	40825.0
68	40825.0
69	35105.5
70	29386.0
71	25855.0
72	22324.0
73	17692.0
74	13060.0
75	13060.0
76	10292.0
77	7524.0
78	6227.5
79	4931.0
80	3908.0
81	2885.0
82	2885.0
83	2266.5
84	1648.0
85	1417.5
86	1187.0
87	1024.0
88	861.0
89	861.0
90	643.0
91	425.0
92	238.0
93	51.0
94	33.5
95	16.0
96	16.0
97	9.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1907618.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.27565372825257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.4845625986234	45.5424595611539
2	8.789640360188978	9.365476724506022
3	2.2573165940561926	3.6078005165992852
4	0.9511858997161374	2.0270020249789322
5	0.5116533764673619	1.3629334056783209
6	0.33514400118819276	1.0713009453841935
7	0.2216517184046079	0.8266048138597225
8	0.1737478283732289	0.74052233123584
9	0.13022633386154722	0.6244103762196841
>10	0.8723903215888396	9.05144495946599
>50	0.12536082897159107	4.72660101916556
>100	0.13509878721514676	14.520585762338175
>500	0.00955795967587169	3.370527397102078
>1k	0.0020692490019928404	1.414859271823431
>5k	2.9560700028469146E-4	1.2026198819457916
>10k+	9.853566676156381E-5	0.5448510085431653
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10379	0.5440816767298274	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8784	0.46046954893484965	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8137	0.4265529052462285	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5988	0.3138993236591393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.242139673666321E-5	0.0
2	0.0	0.0	0.0	0.0016774846955732226	0.0
3	0.0	0.0	0.0	0.002568648440096497	0.0
4	0.0	0.0	0.0	0.004613082912826362	0.0
5	0.0	0.0	0.0	0.005294561070402984	0.0
6	0.0	0.0	0.0	0.005346982467139647	0.0
7	0.0	0.0	0.0	0.0061857248149262585	0.0
8	0.0	0.0	0.0	0.0068147815757662175	0.0
9	0.0	0.0	0.0	0.011689971472275896	0.0
10	0.0	0.0	0.0	0.017666010700255502	0.0
11	0.0	0.0	0.0	0.020234659140352	0.0
12	0.0	0.0	0.0	0.021912143835925223	0.0
13	0.0	0.0	0.0	0.023275100151078466	0.0
14	0.0	0.0	0.0	0.025371956020544994	0.0
15	0.0	0.0	0.0	0.028464818428008125	0.0
16	0.0	0.0	0.0	0.033077901340834485	0.0
17	0.0	0.0	0.0	0.03826761961776414	0.0
18	0.0	0.0	0.0	0.0408886894545973	0.0
19	0.0	0.0	0.0	0.04508240119353036	0.0
20	0.0	0.0	0.0	0.049538219916146736	0.0
21	0.0	0.0	0.0	0.0541513028289731	0.0
22	0.0	0.0	0.0	0.06447831798609575	0.0
23	0.0	0.0	0.0	0.07816030253436484	0.0
24	0.0	0.0	0.0	0.09787074770735021	0.0
25	0.0	0.0	0.0	0.10494763626679975	0.0
26	0.0	0.0	0.0	0.11663760773907564	0.0
27	0.0	0.0	0.0	0.13661015989574432	0.0
28	0.0	0.0	0.0	0.17178491710604535	0.0
29	0.0	0.0	0.0	0.22279093613081866	0.0
30	0.0	0.0	0.0	0.2971244767034071	0.0
31	0.0	0.0	0.0	0.5479084386916039	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	105	4.0017767E-11	22.90476	15
TATCCCG	45	0.0038267204	20.555555	5
AGTCGGT	285	0.0	18.175438	11
CAGTCGG	290	0.0	17.224138	10
AGACGTA	70	0.0025933343	15.857143	5
GCAGTCG	320	0.0	15.609375	9
CGGTGAT	350	0.0	15.328572	14
GGTATCA	5810	0.0	14.806369	1
TGCGTTA	75	0.004105725	14.8	8
TCGGTGA	375	0.0	14.306667	13
CGGACCA	285	0.0	14.280702	9
TCTAGCG	210	1.364242E-10	14.095238	28
AATGCGT	105	1.656535E-4	14.095238	6
CGCAATA	210	1.364242E-10	14.095238	36
GTCGGTG	370	0.0	14.0	12
TCTATGG	530	0.0	13.962264	2
AAGACGG	305	0.0	13.95082	5
CTTATAC	1285	0.0	13.677043	37
TAGGACA	800	0.0	13.64375	4
CAAGACG	340	0.0	13.6029415	4
>>END_MODULE