FastQCFastQC Report
Fri 10 Feb 2017
ERR1630551.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630551.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130684
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45003.4434207707140887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41993.213094181384102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29882.286431391754155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17631.3490557375042087No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT17491.3383428728842093No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT13020.9962964096599431TruSeq Adapter, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG7530.5761990756328242No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC6950.5318172079213982No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC5840.4468794955771173TruSeq Adapter, Index 11 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.36882862477426465No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4810.36806342015855037No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4560.34893330476569434No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.3083774601328395No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3750.28695173089284076No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.25098711395427137No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.24639588625998593No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA2900.22190933855713016No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC2870.21961372470998744RNA PCR Primer, Index 11 (95% over 24bp)
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA2750.21043126932141654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2690.2058400416271311No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2390.18288390315570383No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2290.17523185699856142No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA2060.15763215083713383No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG2020.1545713323742769No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1990.15227571852713415No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.14232805852284902No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1820.13926724005999203No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1810.1385020354442778No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.12702396620856418No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1630.12472835236142144No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1560.11937192005142175No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.11171987389427933No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA1460.11171987389427933No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1450.11095466927856508No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1390.10636344158427963No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1370.10483303235285116No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1360.10406782773713691No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1340.10253741850570844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCT200.00183597237.035
CCGTCTT1900.031.15789637
CGTCTTC250.00547875129.637
GCCGTCT2000.029.636
ATGCCCC358.827208E-426.42857237
AGCTGAT509.01083E-625.9000027
TGCCGTC2400.024.66666635
ATGCCGT2450.024.16326734
GAGCTGA551.8859793E-523.5454546
GCTGATG551.8859793E-523.5454548
TATGCCG2550.023.21568733
CAGTCGG2500.022.93999910
TGGCGTT502.684381E-422.213
AGTCGGT2600.022.05769211
GGGCAGT2650.021.641517
GCAGTCG2700.021.240749
GGCAGTC2750.020.8545468
GTCGGTG2850.020.77192912
GCGTTTT450.00380795620.55555715
GTGATTC2700.020.55555316
Produced by FastQC (version 0.11.5)