##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630551.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130684
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91569740748676	33.0	31.0	34.0	30.0	34.0
2	31.993832450797342	33.0	31.0	34.0	30.0	34.0
3	31.93986256925102	33.0	31.0	34.0	30.0	34.0
4	35.686105414587864	37.0	35.0	37.0	33.0	37.0
5	35.715259710446574	37.0	35.0	37.0	35.0	37.0
6	35.86364818952588	37.0	35.0	37.0	35.0	37.0
7	35.775634354626426	37.0	35.0	37.0	35.0	37.0
8	35.84811453582688	37.0	35.0	37.0	35.0	37.0
9	37.42957056716966	39.0	37.0	39.0	35.0	39.0
10	37.37377184659178	39.0	37.0	39.0	34.0	39.0
11	37.5144088029139	39.0	37.0	39.0	35.0	39.0
12	37.47725811882097	39.0	37.0	39.0	35.0	39.0
13	37.54621835878914	39.0	37.0	39.0	35.0	39.0
14	38.56739922255211	40.0	38.0	41.0	34.0	41.0
15	38.49511034250558	40.0	38.0	41.0	34.0	41.0
16	38.324033546570355	40.0	38.0	41.0	34.0	41.0
17	38.50931254017324	40.0	38.0	41.0	34.0	41.0
18	38.666034097517674	40.0	38.0	41.0	35.0	41.0
19	38.805094732331426	40.0	38.0	41.0	35.0	41.0
20	38.83381286155918	40.0	38.0	41.0	35.0	41.0
21	38.80161305132992	40.0	38.0	41.0	35.0	41.0
22	38.637629702182366	40.0	38.0	41.0	34.0	41.0
23	38.546868782712494	40.0	38.0	41.0	34.0	41.0
24	38.44748400722353	40.0	38.0	41.0	34.0	41.0
25	38.35683786844602	40.0	37.0	41.0	34.0	41.0
26	38.099866854396865	40.0	37.0	41.0	34.0	41.0
27	37.84859661473478	40.0	36.0	41.0	33.0	41.0
28	37.69918276147042	40.0	36.0	41.0	33.0	41.0
29	37.458235132074314	39.0	35.0	41.0	33.0	41.0
30	37.19069664228215	39.0	35.0	41.0	33.0	41.0
31	36.857411771907806	39.0	35.0	41.0	32.0	41.0
32	36.47461816289676	38.0	35.0	40.0	31.0	41.0
33	36.203284258210644	38.0	35.0	40.0	31.0	41.0
34	36.02274188117903	38.0	35.0	40.0	30.0	41.0
35	35.75716996724924	38.0	35.0	40.0	30.0	41.0
36	35.52751675798108	38.0	35.0	40.0	29.0	41.0
37	35.15307153132748	38.0	35.0	40.0	26.0	41.0
38	34.754859049309786	37.0	35.0	40.0	24.0	41.0
39	34.383688898411435	37.0	34.0	40.0	22.0	41.0
40	33.873725934314834	37.0	33.0	40.0	18.0	41.0
41	33.506764408802916	36.0	33.0	40.0	15.0	41.0
42	33.02021670594717	35.0	33.0	40.0	12.0	41.0
43	32.100555538551006	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	0.0
13	0.0
14	1.0
15	2.0
16	3.0
17	4.0
18	7.0
19	24.0
20	24.0
21	64.0
22	128.0
23	219.0
24	322.0
25	576.0
26	911.0
27	1360.0
28	1899.0
29	2533.0
30	3193.0
31	3745.0
32	4383.0
33	5594.0
34	7000.0
35	8721.0
36	12375.0
37	22744.0
38	26315.0
39	28530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.61344923632579	17.194147715099017	16.078479385387652	18.113923663187535
2	18.013681858528972	23.909583422607206	34.23295889320804	23.84377582565578
3	21.929999081754463	27.405803311805577	34.12200422392948	16.542193382510483
4	11.913470662055033	18.82173793272321	28.947690612469774	40.31710079275198
5	10.688378072296532	42.65862691683756	31.153775519573934	15.49921949129197
6	28.731902910838357	30.25695570995684	18.901319212757492	22.10982216644731
7	22.836766551375838	37.11624988521931	19.762939610051728	20.284043953353127
8	35.24379419056656	27.69505065654556	19.37727648373175	17.683878669156133
9	27.930733678185547	10.06014508279514	20.467693061124546	41.541428177894765
10	21.202289492210216	30.569159193168254	23.238498974625816	24.990052339995714
11	27.953689816656972	24.800281595298582	17.75351228918613	29.492516298858312
12	20.951302378255946	34.784671421138015	25.61369410180282	18.650332098803222
13	33.99038903002663	17.745860243028986	29.17801720179976	19.085733525144626
14	24.465887178231462	23.226255700774388	29.855988491322577	22.451868629671573
15	30.529368553151116	22.71280340363013	27.07982614551131	19.678001897707446
16	21.873373940191605	23.23390774693153	31.698601205962472	23.194117106914387
17	16.38456123167335	32.82880842337241	29.337944966484038	21.448685378470202
18	18.667931804964645	20.259557405650273	35.03489333047657	26.037617458908514
19	19.91521532857886	26.878577331578462	36.41685286645649	16.789354473386183
20	21.763184475528757	20.434024058033117	39.307795904624896	18.49499556181323
21	26.89541183312418	20.936763490557375	34.470172324079456	17.697652352238986
22	24.507208227480028	23.495607725505803	33.117290563496674	18.879893483517492
23	20.83269566282024	24.13225796578005	33.79143582994092	21.243610541458786
24	19.88537234856601	26.188362768204215	36.81169844816504	17.114566435064738
25	17.636435982981848	26.88699458235132	34.7892626488323	20.687306785834533
26	21.781549386305898	23.734351565608645	34.89333047656943	19.590768571516023
27	18.593706957240368	27.63919071959842	34.33779192556089	19.429310397600318
28	17.34259741054758	25.3060818462857	37.773560650117844	19.577760093048884
29	19.222705151357474	26.175354289737072	37.49273055615072	17.109210002754736
30	17.875945027700407	27.295613847142725	37.938079642496405	16.890361482660463
31	16.529950108659055	28.66533010927122	35.308071378286556	19.49664840378317
32	16.61947904869762	29.10226194484405	37.17823145909216	17.100027547366164
33	15.079122157264852	27.766214685806982	38.39031557038352	18.76434758654464
34	16.62789629947048	29.81237182822687	35.346331609072266	18.213400263230387
35	15.092895840347708	30.40770101925255	36.5033209880322	17.996082152367542
36	15.57497474824768	29.52771571118117	32.91221572648526	21.985093814085886
37	15.077591748033425	30.88059747176395	34.441094548682315	19.60071623152031
38	15.483150194361972	27.809831348902698	35.45575586911941	21.25126258761593
39	16.871996571883322	28.184781610602677	35.063205901258	19.880015916256006
40	15.046983563404853	27.942211747421258	37.497321783845	19.513482905328885
41	14.359829818493466	30.180435248385418	34.34008753940804	21.119647393713077
42	14.870221297174865	28.861222490894068	36.5461724465122	19.722383765418876
43	13.301551834960668	30.70307000091825	35.99369471396651	20.001683450154573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	31.0
2	56.0
3	213.0
4	370.0
5	370.0
6	637.0
7	904.0
8	966.0
9	1028.0
10	1337.5
11	1647.0
12	1647.0
13	2905.5
14	4164.0
15	6532.0
16	8900.0
17	7668.0
18	6436.0
19	6436.0
20	6877.0
21	7318.0
22	4912.0
23	2506.0
24	2430.5
25	2355.0
26	2355.0
27	2402.0
28	2449.0
29	2531.5
30	2614.0
31	2690.5
32	2767.0
33	2767.0
34	2651.5
35	2536.0
36	2562.0
37	2588.0
38	3031.5
39	3475.0
40	3475.0
41	4131.0
42	4787.0
43	5466.0
44	6145.0
45	7717.0
46	9289.0
47	9289.0
48	10487.5
49	11686.0
50	10329.0
51	8972.0
52	8463.0
53	7954.0
54	7954.0
55	6049.5
56	4145.0
57	5052.0
58	5959.0
59	5253.0
60	4547.0
61	4547.0
62	3897.5
63	3248.0
64	3086.5
65	2925.0
66	2768.0
67	2611.0
68	2611.0
69	2399.0
70	2187.0
71	1927.0
72	1667.0
73	1395.0
74	1123.0
75	1123.0
76	912.5
77	702.0
78	513.0
79	324.0
80	246.5
81	169.0
82	169.0
83	122.0
84	75.0
85	55.5
86	36.0
87	23.0
88	10.0
89	10.0
90	7.0
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	130684.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.04031097915583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.34497698348227	39.55878301857917
2	9.305363087558337	8.940650730005203
3	3.1076281040442173	4.478742615775459
4	1.4574473168633824	2.8006488935141256
5	0.8712827129226994	2.092834623978452
6	0.5559006705850497	1.602338465305623
7	0.31538204233764994	1.0605735973799393
8	0.2548541756263838	0.9794619081142297
9	0.2389257896497348	1.0330262312142267
>10	1.3236488746595307	12.068807199045024
>50	0.14176263519217597	4.589697285054024
>100	0.06849205969959064	6.612898289002479
>500	0.004778515792994696	1.5548957791313398
>1k	0.009557031585989391	12.626641363900706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4500	3.4434207707140887	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4199	3.213094181384102	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2988	2.286431391754155	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1763	1.3490557375042087	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	1749	1.3383428728842093	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	1302	0.9962964096599431	TruSeq Adapter, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	753	0.5761990756328242	No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	695	0.5318172079213982	No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	584	0.4468794955771173	TruSeq Adapter, Index 11 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	482	0.36882862477426465	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	481	0.36806342015855037	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	456	0.34893330476569434	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	403	0.3083774601328395	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	375	0.28695173089284076	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	328	0.25098711395427137	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	322	0.24639588625998593	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	290	0.22190933855713016	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	287	0.21961372470998744	RNA PCR Primer, Index 11 (95% over 24bp)
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	275	0.21043126932141654	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	269	0.2058400416271311	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	239	0.18288390315570383	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	229	0.17523185699856142	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	206	0.15763215083713383	No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	202	0.1545713323742769	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	199	0.15227571852713415	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	186	0.14232805852284902	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	182	0.13926724005999203	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	181	0.1385020354442778	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	166	0.12702396620856418	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	163	0.12472835236142144	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	156	0.11937192005142175	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146	0.11171987389427933	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	146	0.11171987389427933	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	145	0.11095466927856508	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	139	0.10636344158427963	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	137	0.10483303235285116	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	136	0.10406782773713691	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	134	0.10253741850570844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.006886841541428178	0.0
3	0.0	0.0	0.0	0.009947660004285146	0.0
4	0.0	0.0	0.0	0.012243273851427872	0.0
5	0.0	0.0	0.0	0.013008478467142114	0.0
6	0.0	0.0	0.0	0.014538887698570598	0.0
7	0.0	0.0	0.0	0.017599706161427565	0.0
8	0.0	0.0	0.0	0.017599706161427565	0.0
9	0.0	0.0	0.0	0.024486547702855743	0.0
10	0.0	0.0	0.0	0.028312570781426953	0.0
11	0.0	0.0	0.0	0.03137338924428392	7.65204615714242E-4
12	0.0	0.0	0.0	0.036729821554283615	7.65204615714242E-4
13	0.0	0.0	0.0	0.040555844632854825	7.65204615714242E-4
14	0.0	0.0	0.0	0.044381867711426035	0.001530409231428484
15	0.0	0.0	0.0	0.052033913868568454	0.001530409231428484
16	0.0	0.0	0.0	0.0857029169599951	0.001530409231428484
17	0.0	0.0	0.0	0.12090232928285023	0.001530409231428484
18	0.0	0.0	0.0	0.1346760123657066	0.001530409231428484
19	0.0	7.65204615714242E-4	0.0	0.16451899237856202	0.001530409231428484
20	0.0	7.65204615714242E-4	0.0	0.17905788007713264	0.001530409231428484
21	0.0	7.65204615714242E-4	0.0	0.23644822625570078	0.001530409231428484
22	0.0	7.65204615714242E-4	0.0	0.3412812586085519	0.001530409231428484
23	0.0	7.65204615714242E-4	0.0	0.5256955709956842	0.001530409231428484
24	0.0	7.65204615714242E-4	0.0	0.7713262526399559	0.001530409231428484
25	0.0	7.65204615714242E-4	0.0	0.8447858957485231	0.001530409231428484
26	0.0	7.65204615714242E-4	0.0	0.9304888127085182	0.001530409231428484
27	0.0	7.65204615714242E-4	0.0	0.9978268188913716	0.001530409231428484
28	0.0	7.65204615714242E-4	0.0	1.0666952343056533	0.001530409231428484
29	0.0	7.65204615714242E-4	0.0	1.160815402038505	0.002295613847142726
30	0.0	7.65204615714242E-4	0.0	1.4110373113770622	0.002295613847142726
31	0.0	7.65204615714242E-4	0.0	2.1869547917113037	0.002295613847142726
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCT	20	0.001835972	37.0	35
CCGTCTT	190	0.0	31.157896	37
CGTCTTC	25	0.005478751	29.6	37
GCCGTCT	200	0.0	29.6	36
ATGCCCC	35	8.827208E-4	26.428572	37
AGCTGAT	50	9.01083E-6	25.900002	7
TGCCGTC	240	0.0	24.666666	35
ATGCCGT	245	0.0	24.163267	34
GAGCTGA	55	1.8859793E-5	23.545454	6
GCTGATG	55	1.8859793E-5	23.545454	8
TATGCCG	255	0.0	23.215687	33
CAGTCGG	250	0.0	22.939999	10
TGGCGTT	50	2.684381E-4	22.2	13
AGTCGGT	260	0.0	22.057692	11
GGGCAGT	265	0.0	21.64151	7
GCAGTCG	270	0.0	21.24074	9
GGCAGTC	275	0.0	20.854546	8
GTCGGTG	285	0.0	20.771929	12
GCGTTTT	45	0.003807956	20.555557	15
GTGATTC	270	0.0	20.555553	16
>>END_MODULE