FastQCFastQC Report
Fri 10 Feb 2017
ERR1630550.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630550.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448848
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60381.3452215449328058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59811.332522368374149No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45961.0239546572559084No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37290.8307934980216019No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.23460057747834456No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.20541475065055428No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9150.20385520265212279No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6740.15016219299183686No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6570.14637471928136028No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT6310.14058211243004312No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5540.12342708444729618No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5540.12342708444729618No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5510.12275870673368267No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.12142195130645564No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC4720.10515809360852671No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.1007022421844366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC358.860938E-426.428573
ACTAGAC451.321457E-424.6666683
TATACTG551.8993102E-523.5454545
CCGCTTA400.001929363723.12525
CCGTCGT450.00382222920.5555579
ACCGTCG450.00382222920.5555578
ACACTAT450.00382222920.5555576
ACCCGCT450.00382222920.55555736
TGCCCTA555.1363825E-420.1818185
GCCCTAT555.1363825E-420.1818186
ATACACT851.2418495E-619.5882364
TCGATGG609.224859E-418.519
GTTACAG609.224859E-418.523
TCTAATA701.21709716E-418.52
AACGCTA609.224859E-418.523
CTAATAC902.146273E-618.53
TCTAGTA500.00702836618.4999982
TCTCGTC500.00702836618.49999812
TAGGGGT500.00702836618.4999984
AGACACT500.00702836618.4999986
Produced by FastQC (version 0.11.5)