##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630550.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448848
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.102736338359534	31.0	31.0	33.0	28.0	34.0
2	31.24351005240081	31.0	31.0	34.0	28.0	34.0
3	31.282153423876235	31.0	31.0	34.0	28.0	34.0
4	35.09674990197127	37.0	35.0	37.0	32.0	37.0
5	34.78031092931237	35.0	35.0	37.0	32.0	37.0
6	34.92121831889637	35.0	35.0	37.0	32.0	37.0
7	34.860861583431365	35.0	35.0	37.0	32.0	37.0
8	34.9108094499697	35.0	35.0	37.0	32.0	37.0
9	36.374126653120875	38.0	35.0	39.0	32.0	39.0
10	36.15025799379745	37.0	35.0	39.0	32.0	39.0
11	36.38334580971732	38.0	35.0	39.0	32.0	39.0
12	36.30710619185114	38.0	35.0	39.0	32.0	39.0
13	36.42485206573272	38.0	35.0	39.0	32.0	39.0
14	37.36693045307097	39.0	36.0	40.0	32.0	41.0
15	37.31205441485759	39.0	36.0	40.0	32.0	41.0
16	37.193016344063025	39.0	36.0	40.0	32.0	41.0
17	37.24941851138916	39.0	36.0	40.0	32.0	41.0
18	37.264191886785724	39.0	36.0	40.0	32.0	41.0
19	37.367159929419316	39.0	36.0	40.0	32.0	41.0
20	37.34937439846006	39.0	36.0	40.0	32.0	41.0
21	37.314222186575414	39.0	36.0	40.0	32.0	41.0
22	37.21886473817417	39.0	36.0	40.0	32.0	41.0
23	37.097010569279576	39.0	36.0	40.0	32.0	41.0
24	37.00404145724165	39.0	36.0	40.0	31.0	41.0
25	36.91302400812747	38.0	36.0	40.0	31.0	41.0
26	36.64466590026022	38.0	35.0	40.0	31.0	41.0
27	36.408472355897764	38.0	35.0	40.0	30.0	41.0
28	36.22825098919902	38.0	35.0	40.0	30.0	41.0
29	36.04999242505258	38.0	35.0	40.0	30.0	41.0
30	35.84190416354757	38.0	35.0	40.0	29.0	41.0
31	35.68551269026486	38.0	34.0	40.0	29.0	41.0
32	35.4486529961145	38.0	34.0	40.0	28.0	41.0
33	35.26035317078387	38.0	34.0	40.0	27.0	41.0
34	35.09914269418601	38.0	34.0	40.0	27.0	41.0
35	34.92351085445407	38.0	34.0	40.0	26.0	41.0
36	34.760228406944	37.0	33.0	40.0	25.0	41.0
37	34.45785210137953	37.0	33.0	40.0	24.0	41.0
38	34.13796207179268	37.0	33.0	40.0	23.0	41.0
39	33.892297615228316	37.0	33.0	40.0	21.0	41.0
40	33.51420971019142	37.0	33.0	40.0	19.0	41.0
41	33.26267912522725	37.0	33.0	40.0	18.0	41.0
42	32.905152746586815	36.0	32.0	40.0	15.0	41.0
43	32.109859016860945	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	2.0
13	3.0
14	2.0
15	14.0
16	13.0
17	35.0
18	59.0
19	137.0
20	274.0
21	501.0
22	898.0
23	1374.0
24	2136.0
25	3277.0
26	4462.0
27	6399.0
28	8635.0
29	11107.0
30	13776.0
31	17053.0
32	21003.0
33	26502.0
34	33531.0
35	45103.0
36	63614.0
37	82570.0
38	78049.0
39	28315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.85104088689267	21.841915303176133	13.210262716999965	20.096781092931238
2	18.226437457669412	24.295529889851352	34.13828289309521	23.339749759384024
3	22.500712936227853	27.380984208462557	29.814324671158165	20.30397818415143
4	14.3097440558942	17.218301072969023	34.50767297615228	33.964281894984495
5	11.894449791466153	41.633247780986	33.141063344383845	13.331239083164013
6	31.080677645884574	35.08737924642641	17.34128257227391	16.49066053541511
7	24.99398460057748	34.539755106405735	23.326604997682956	17.13965529533383
8	28.53772323815635	32.3575018714576	21.06637436281325	18.038400527572808
9	26.905544861512137	13.236552240402096	23.522216875200513	36.335686022885255
10	18.00542722703454	29.81900331515346	32.05962820375717	20.115941254054825
11	30.1523010016754	24.729752611128937	22.143576444587033	22.974369942608632
12	19.953748262217943	30.23963568958757	30.175025843938258	19.63159020425623
13	28.303345453249207	21.54025416176523	29.462757993797457	20.693642391188106
14	21.293399992870636	24.19304530709728	30.146508394824085	24.367046305208
15	25.048568780522583	27.991658646134105	27.72854061954158	19.231231953801732
16	21.015577656578618	28.110407086586104	30.18482871707126	20.68918653976402
17	19.778633301251205	29.21434427690443	30.437252700245963	20.5697697215984
18	18.95964780950344	25.864212383702277	33.359177271593055	21.816962535201228
19	19.34975760168253	28.030647346094888	33.94133426015043	18.678260792072148
20	20.46193811713542	25.960013189320215	34.44484547107261	19.13320322247175
21	21.537580650910776	27.118757352154848	32.88596585035469	18.45769614657969
22	21.465395857840516	26.994439097422735	32.67698641856486	18.86317862617189
23	20.44255516344063	26.912897016361885	33.074671158164904	19.56987666203258
24	19.11203792820732	28.39313085944462	32.82803978184151	19.666791430506542
25	19.403227818771608	28.04200976722632	33.067541795886356	19.48722061811571
26	19.327701137133282	28.672958328877478	32.6442359105978	19.355104623391437
27	19.39097422735536	28.693678037999497	32.91537446975369	18.999973264891455
28	19.047650875129218	28.54819448900296	33.41554379210779	18.988610843760025
29	18.464379923715825	29.087129718746656	33.84196877339322	18.606521584144296
30	18.11816026806402	30.031993013224966	33.28922040423484	18.56062631447617
31	18.991729939756887	29.93374148932378	32.413868391972336	18.660660178946994
32	18.085409760096958	29.688669660998823	32.918716358321745	19.30720422058247
33	17.722926246747228	29.87002281395929	32.98867322567997	19.418377713613516
34	18.65509036466688	29.630075214772038	32.55823797811286	19.156596442448222
35	18.031493957865468	30.11598581256907	32.64891455459309	19.203605674972373
36	18.332041136420347	30.105068976580046	31.67575660357181	19.887133283427797
37	18.790325455388015	28.877927494385624	32.50164866502691	19.83009838519944
38	18.321124300431325	28.678082558015184	32.33923288061883	20.661560260934657
39	18.344294727836594	29.526031084019532	32.72288168823299	19.406792499910882
40	17.85949809289559	29.10294799130218	32.66049976829572	20.377054147506506
41	17.789095640394965	29.39725697786333	32.399832459986456	20.41381492175525
42	17.864176736890887	29.42443767155028	32.358170249171216	20.353215342387625
43	17.182208676433895	28.881269382953693	32.00548960895448	21.93103233165793
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	194.0
1	179.5
2	165.0
3	437.0
4	709.0
5	709.0
6	1237.5
7	1766.0
8	2026.5
9	2287.0
10	3164.5
11	4042.0
12	4042.0
13	7238.0
14	10434.0
15	14026.5
16	17619.0
17	16140.0
18	14661.0
19	14661.0
20	15842.0
21	17023.0
22	14203.0
23	11383.0
24	11789.0
25	12195.0
26	12195.0
27	12656.5
28	13118.0
29	14033.0
30	14948.0
31	16327.0
32	17706.0
33	17706.0
34	18918.0
35	20130.0
36	21023.5
37	21917.0
38	22971.0
39	24025.0
40	24025.0
41	24872.5
42	25720.0
43	26137.0
44	26554.0
45	27123.0
46	27692.0
47	27692.0
48	27965.0
49	28238.0
50	26277.5
51	24317.0
52	23269.5
53	22222.0
54	22222.0
55	20561.5
56	18901.0
57	17510.0
58	16119.0
59	14705.0
60	13291.0
61	13291.0
62	12662.0
63	12033.0
64	10779.5
65	9526.0
66	8569.0
67	7612.0
68	7612.0
69	6256.0
70	4900.0
71	4117.0
72	3334.0
73	2576.0
74	1818.0
75	1818.0
76	1342.5
77	867.0
78	675.0
79	483.0
80	394.0
81	305.0
82	305.0
83	262.0
84	219.0
85	192.5
86	166.0
87	137.0
88	108.0
89	108.0
90	94.5
91	81.0
92	47.5
93	14.0
94	8.0
95	2.0
96	2.0
97	2.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	448848.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.7172275907509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.08478366960222	53.79613528861196
2	5.226401263375721	6.24212387451192
3	1.3695663386198105	2.453601142319719
4	0.7462412454658399	1.7825383317235588
5	0.45757970665453956	1.3662695741599087
6	0.3192904208633711	1.1440283238146742
7	0.23599649852444468	0.9865139629103199
8	0.19138291462075144	0.9143085655510929
9	0.148306832413074	0.7970825578027403
>10	1.027935902288703	12.294705038102641
>50	0.11550912454730335	4.747039407901516
>100	0.07177265990605985	7.3590830524096695
>500	0.0033643434330965557	1.340064780792794
>1k	0.0011214478110321852	2.093474515121576
>5k	7.476318740214569E-4	2.683031584265965
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6038	1.3452215449328058	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5981	1.332522368374149	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4596	1.0239546572559084	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3729	0.8307934980216019	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1053	0.23460057747834456	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	922	0.20541475065055428	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	915	0.20385520265212279	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	674	0.15016219299183686	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	657	0.14637471928136028	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	631	0.14058211243004312	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	554	0.12342708444729618	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	554	0.12342708444729618	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	551	0.12275870673368267	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	545	0.12142195130645564	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	472	0.10515809360852671	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	452	0.1007022421844366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.2279257120450576E-4	0.0
3	0.0	0.0	0.0	0.0011139628560225287	0.0
4	0.0	0.0	0.0	0.0017823405696360461	0.0
5	0.0	0.0	0.0	0.0017823405696360461	0.0
6	0.0	0.0	0.0	0.0017823405696360461	0.0
7	0.0	0.0	0.0	0.002005133140840552	0.0
8	0.0	0.0	0.0	0.0024507182832495633	0.0
9	0.0	0.0	0.0	0.004901436566499127	0.0
10	0.0	0.0	0.0	0.008243325134566713	0.0
11	0.0	0.0	0.0	0.010916835989020782	0.0
12	0.0	0.0	0.0	0.01626385769792892	0.0
13	0.0	0.0	0.0	0.019828538837201013	0.0
14	0.0	0.0	0.0	0.024952767974904644	0.0
15	0.0	0.0	0.0	0.03386447082308487	0.0
16	0.0	0.0	0.0	0.050796706234627315	0.0
17	0.0	0.0	0.0	0.07530388906712294	0.0
18	0.0	0.0	0.0	0.08443838448650769	0.0
19	0.0	0.0	0.0	0.10114782732684562	0.0
20	0.0	0.0	0.0	0.11317862617188892	0.0
21	0.0	0.0	0.0	0.14080490500124765	0.0
22	2.2279257120450576E-4	0.0	0.0	0.19182440380707946	0.0
23	2.2279257120450576E-4	0.0	0.0	0.26668270773179337	0.0
24	2.2279257120450576E-4	0.0	0.0	0.3912237550351121	0.0
25	2.2279257120450576E-4	0.0	0.0	0.4221919224325384	0.0
26	2.2279257120450576E-4	0.0	0.0	0.4716518732399387	0.0
27	2.2279257120450576E-4	0.0	0.0	0.5168787651944533	0.0
28	2.2279257120450576E-4	0.0	0.0	0.5777011371332834	0.0
29	2.2279257120450576E-4	0.0	0.0	0.657683670195701	0.0
30	2.2279257120450576E-4	0.0	0.0	0.812301714611628	0.0
31	2.2279257120450576E-4	0.0	0.0	1.2440737176059602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACAC	35	8.860938E-4	26.42857	3
ACTAGAC	45	1.321457E-4	24.666668	3
TATACTG	55	1.8993102E-5	23.545454	5
CCGCTTA	40	0.0019293637	23.125	25
CCGTCGT	45	0.003822229	20.555557	9
ACCGTCG	45	0.003822229	20.555557	8
ACACTAT	45	0.003822229	20.555557	6
ACCCGCT	45	0.003822229	20.555557	36
TGCCCTA	55	5.1363825E-4	20.181818	5
GCCCTAT	55	5.1363825E-4	20.181818	6
ATACACT	85	1.2418495E-6	19.588236	4
TCGATGG	60	9.224859E-4	18.5	19
GTTACAG	60	9.224859E-4	18.5	23
TCTAATA	70	1.21709716E-4	18.5	2
AACGCTA	60	9.224859E-4	18.5	23
CTAATAC	90	2.146273E-6	18.5	3
TCTAGTA	50	0.007028366	18.499998	2
TCTCGTC	50	0.007028366	18.499998	12
TAGGGGT	50	0.007028366	18.499998	4
AGACACT	50	0.007028366	18.499998	6
>>END_MODULE