FastQCFastQC Report
Fri 10 Feb 2017
ERR1630549.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630549.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219282
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC95690.4311754882885546No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT73660.3319091489950353No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA66850.30122354887751984No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA65790.2964472293291254No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG57240.2579212556133019No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG56660.2553077977471993No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG56470.2544516649979588No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG55690.2509370147642346No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG55170.24859391460841843No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG47840.21556521433508677No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG45910.20686870798753831No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA41720.1879887278858658No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG41630.18758319132043608No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA40450.18226615635146862No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA40180.18104954665517947No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC37710.1699198209150527No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT35310.1591055125035935No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA35020.1577987835705422No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG34570.1557711007433936No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA34260.15437425257358012No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC33070.14901215798623157No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA30640.13806267071962913No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA29830.13441284163076167No Hit
GGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTC28880.13013217788455908No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG28460.1282396739125537No Hit
GTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTC28380.12787919696550507No Hit
TGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGT27640.12454478520530514No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC26790.12071471764291335No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC26540.11958822718338635No Hit
GAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCAT26370.11882221367090799No Hit
GTAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAG26340.11868703481576474No Hit
GCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCC25990.11710994817242693No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA25290.11395577488575133No Hit
TAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAGG25120.11318976137327298No Hit
CCACTGGAGCCAGTGTACCCAGGGGACAATGCCACACCAGAGC25030.11278422480784325No Hit
GCCTGAGGCAGGTGCTCGCTTGGTCTAGTGCCCATTTACTCTG24770.11161267472993518No Hit
AGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCTCCCTG24300.10949487266602442No Hit
GAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACAGAAGG24240.10922451495573794No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG24050.10836838220649743No Hit
TCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTG23640.10652093785287313No Hit
GCTCTGGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGG23620.10643081861611096No Hit
CGCTTGGTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGC23580.10625058014258665No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC23480.10579998395877585No Hit
CTTCTGTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCT23440.10561974548525153No Hit
GAGCAAGCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTG23310.10503397044629749No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT23290.10494385120953532No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG23030.10377230113162725No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC22560.1016544990677165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.021.3121
GCTTTAT15550.019.8681661
CTAGTAC650.001580560117.0769253
CTTTATT18500.017.02
ATCTAGG1005.88275E-616.6500021
TTGCCGA1005.88275E-616.65000210
TTTATTG18750.016.4773353
CGAATTA803.3843415E-416.187515
CTTATAC9200.016.08695637
TATTGAG18850.015.8992045
TTAGACT1659.749783E-1015.6969714
TTATTGA19100.015.594244
ACGTTCG957.0644106E-515.5789487
TTTCGGA3000.015.41666730
AAGACGG2550.015.2352945
GTTATAC1101.4530004E-515.1363643
CGCAAGA2950.015.0508472
TCTGTCG1252.9615658E-614.8000018
ATATCGC750.004106006614.86
TATAGAG1508.1114194E-814.85
Produced by FastQC (version 0.11.5)