##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630549.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2219282
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1493748879142	33.0	31.0	34.0	30.0	34.0
2	32.341206750651786	34.0	31.0	34.0	30.0	34.0
3	32.45876549262329	34.0	31.0	34.0	30.0	34.0
4	36.008473911832745	37.0	35.0	37.0	35.0	37.0
5	35.95076290439881	37.0	35.0	37.0	35.0	37.0
6	36.02247618824467	37.0	35.0	37.0	35.0	37.0
7	35.978583163383476	37.0	35.0	37.0	35.0	37.0
8	35.9679612595425	37.0	35.0	37.0	35.0	37.0
9	37.70312740787336	39.0	37.0	39.0	35.0	39.0
10	37.6328587353928	39.0	37.0	39.0	35.0	39.0
11	37.70438321943764	39.0	37.0	39.0	35.0	39.0
12	37.656360931148	39.0	37.0	39.0	35.0	39.0
13	37.691215447158136	39.0	37.0	39.0	35.0	39.0
14	39.028479030605396	40.0	38.0	41.0	36.0	41.0
15	39.05076371547194	40.0	38.0	41.0	36.0	41.0
16	39.02439077142968	40.0	38.0	41.0	36.0	41.0
17	39.010982831384204	40.0	38.0	41.0	36.0	41.0
18	38.984044389131256	40.0	38.0	41.0	36.0	41.0
19	39.006930619903194	40.0	38.0	41.0	36.0	41.0
20	38.98858054091368	40.0	38.0	41.0	36.0	41.0
21	38.94282159725533	40.0	38.0	41.0	35.0	41.0
22	38.88574998580622	40.0	38.0	41.0	35.0	41.0
23	38.86748236591835	40.0	38.0	41.0	35.0	41.0
24	38.820451839829275	40.0	38.0	41.0	35.0	41.0
25	38.77307435467868	40.0	38.0	41.0	35.0	41.0
26	38.66209206400989	40.0	38.0	41.0	35.0	41.0
27	38.54671871352987	40.0	38.0	41.0	34.0	41.0
28	38.46352829428617	40.0	38.0	41.0	34.0	41.0
29	38.42984352596921	40.0	38.0	41.0	34.0	41.0
30	38.38151798644787	40.0	38.0	41.0	34.0	41.0
31	38.36440659636765	40.0	38.0	41.0	34.0	41.0
32	38.32101823923233	40.0	38.0	41.0	34.0	41.0
33	38.25931900497548	40.0	38.0	41.0	34.0	41.0
34	38.228499577791375	40.0	38.0	41.0	34.0	41.0
35	38.17295999336722	40.0	38.0	41.0	33.0	41.0
36	38.17220704714408	40.0	38.0	41.0	33.0	41.0
37	38.07313942076762	40.0	38.0	41.0	33.0	41.0
38	37.95461189700092	40.0	37.0	41.0	33.0	41.0
39	37.92623470113307	40.0	37.0	41.0	33.0	41.0
40	37.77520387224337	40.0	37.0	41.0	33.0	41.0
41	37.75824748725038	40.0	37.0	41.0	33.0	41.0
42	37.65267280138351	40.0	37.0	41.0	33.0	41.0
43	36.84680946360129	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	2.0
12	2.0
13	1.0
14	6.0
15	0.0
16	9.0
17	17.0
18	26.0
19	59.0
20	143.0
21	301.0
22	616.0
23	1069.0
24	1896.0
25	3311.0
26	5179.0
27	8118.0
28	12108.0
29	17132.0
30	24163.0
31	32787.0
32	43833.0
33	58506.0
34	79825.0
35	111819.0
36	161007.0
37	254995.0
38	513404.0
39	888942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.58388704094387	17.474390365893115	10.992699440629899	25.94902315253312
2	20.60535794910246	19.21986480312101	31.219241178002616	28.955536069773917
3	22.49367137659838	20.93131922847119	27.039510976973634	29.535498417956795
4	15.703096767332859	13.084502104734774	32.4341386087933	38.778262519139076
5	15.863644187624645	35.110094165590496	33.93178514492525	15.09447650185961
6	39.493043245518145	31.791903868007758	13.503376317205296	15.2116765692688
7	32.00819003623695	27.48839489528595	19.874626117816483	20.62878895066062
8	30.364009621129718	29.45222824318856	18.754669302954742	21.42909283272698
9	27.455636552722908	13.062422891728046	18.06129189530668	41.420648660242364
10	18.70082305898935	25.173051464392536	31.253937084156046	24.872188392462068
11	38.318293934705004	20.416242730757066	19.619363379687666	21.64609995485026
12	25.70556603442014	23.426901132888926	25.57052235813204	25.29701047455889
13	33.01847173995914	17.913406227779976	22.945844647052517	26.12227738520837
14	24.52189491916755	18.839291266274408	22.764299444595142	33.8745143699629
15	27.650384223365936	26.560031577780563	20.006876097764952	25.782708101088552
16	27.454645241118524	24.444842971735902	21.652408301423616	26.44810348572196
17	27.060013103337027	23.87272099715133	22.31347796269244	26.753787936819208
18	25.684072596452367	23.064351443394756	24.72835809058966	26.52321786956322
19	28.105846845961892	22.581672811296627	24.370855078354168	24.94162526438731
20	28.474885120502936	22.356645077101515	22.53922665078165	26.629243151613903
21	27.338256246840192	22.61015049011347	23.709289761283152	26.34230350176318
22	27.77163965642942	22.881274213912427	23.710100834414014	25.636985295244134
23	26.963225043054468	22.823552842766265	22.804717922282972	27.4085041918963
24	26.373935353866703	23.377425671906497	24.289207049847654	25.959431924379146
25	27.141345714514877	23.60993330275287	22.73870558135469	26.51001540137756
26	27.126025444265306	23.68121761903174	23.20845210297745	25.984304833725503
27	27.18023216517775	21.9672849146706	23.580464312331646	27.27201860782001
28	26.508618553207747	23.400451136899232	22.99644659849447	27.094483711398553
29	27.124718715332257	23.791072968644812	23.67864922078402	25.40555909523891
30	27.17257203005296	23.110222134906692	22.376200951478904	27.341004883561443
31	25.417364715254752	24.514099605187624	24.208685511800663	25.85985016775696
32	25.795279734616873	23.06791115324686	23.240489491646397	27.89631962048987
33	25.037376953447104	24.56231339685538	23.57974335843755	26.82056629125997
34	28.25931990616785	21.927452212021727	23.238777226147917	26.574450655662506
35	26.21284721815434	22.486326658802263	23.62525357300244	27.67557255004096
36	25.183550355475333	24.069631529476652	24.555689632953364	26.191128482094662
37	26.58485942750854	22.08277271658131	24.973617593437876	26.358750262472274
38	26.956330921442163	23.07498551333269	23.346469714078697	26.622213851146455
39	26.24091936040575	22.463886968848485	24.328859514023005	26.96633415672276
40	25.147502660770467	21.999502541813072	24.85979699740727	27.993197800009188
41	23.65021660158556	22.942420116055555	25.846332282242635	27.561031000116255
42	25.133353940598806	22.321273276672365	26.543494697834706	26.001878084894127
43	26.214108887469013	22.072769481300707	25.38866173834601	26.324459892884278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55.0
1	81.0
2	107.0
3	244.5
4	382.0
5	382.0
6	526.0
7	670.0
8	605.5
9	541.0
10	799.5
11	1058.0
12	1058.0
13	1694.0
14	2330.0
15	3283.5
16	4237.0
17	4373.5
18	4510.0
19	4510.0
20	5971.5
21	7433.0
22	8342.5
23	9252.0
24	11576.0
25	13900.0
26	13900.0
27	16839.0
28	19778.0
29	24102.0
30	28426.0
31	33735.5
32	39045.0
33	39045.0
34	46298.5
35	53552.0
36	60991.0
37	68430.0
38	76444.5
39	84459.0
40	84459.0
41	89830.5
42	95202.0
43	99556.5
44	103911.0
45	111568.5
46	119226.0
47	119226.0
48	132753.0
49	146280.0
50	150064.5
51	153849.0
52	157569.5
53	161290.0
54	161290.0
55	156250.5
56	151211.0
57	154361.0
58	157511.0
59	172115.5
60	186720.0
61	186720.0
62	173230.0
63	159740.0
64	151774.5
65	143809.0
66	126570.0
67	109331.0
68	109331.0
69	98586.0
70	87841.0
71	73105.0
72	58369.0
73	41379.5
74	24390.0
75	24390.0
76	16872.0
77	9354.0
78	7468.0
79	5582.0
80	4491.5
81	3401.0
82	3401.0
83	2636.5
84	1872.0
85	1491.5
86	1111.0
87	903.0
88	695.0
89	695.0
90	527.0
91	359.0
92	202.5
93	46.0
94	28.5
95	11.0
96	11.0
97	7.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2219282.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.21607219614955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.95046404570562	40.58243315674593
2	8.55256307969717	8.076368716662097
3	2.1816326329015627	3.0902437170166808
4	0.8905852732060172	1.681997542260915
5	0.5141450912336064	1.2137955873490929
6	0.3308950818043074	0.9374139643093794
7	0.2016965203356752	0.6666322226116971
8	0.16070101968746545	0.6070136758046576
9	0.13007681496039336	0.5527544657593607
>10	0.8367120191590429	7.861923853676017
>50	0.11538337271909763	3.804342981036852
>100	0.10291880602831938	10.25763207474576
>500	0.017647704260017074	5.828530101442732
>1k	0.013715335375991528	12.197770421610512
>5k	8.632029257617047E-4	2.6411475189683697
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	9569	0.4311754882885546	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	7366	0.3319091489950353	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	6685	0.30122354887751984	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	6579	0.2964472293291254	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	5724	0.2579212556133019	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	5666	0.2553077977471993	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	5647	0.2544516649979588	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	5569	0.2509370147642346	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	5517	0.24859391460841843	No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG	4784	0.21556521433508677	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	4591	0.20686870798753831	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	4172	0.1879887278858658	No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG	4163	0.18758319132043608	No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA	4045	0.18226615635146862	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	4018	0.18104954665517947	No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC	3771	0.1699198209150527	No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT	3531	0.1591055125035935	No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA	3502	0.1577987835705422	No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG	3457	0.1557711007433936	No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA	3426	0.15437425257358012	No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC	3307	0.14901215798623157	No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA	3064	0.13806267071962913	No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA	2983	0.13441284163076167	No Hit
GGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTC	2888	0.13013217788455908	No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG	2846	0.1282396739125537	No Hit
GTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTC	2838	0.12787919696550507	No Hit
TGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGT	2764	0.12454478520530514	No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC	2679	0.12071471764291335	No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC	2654	0.11958822718338635	No Hit
GAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCAT	2637	0.11882221367090799	No Hit
GTAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAG	2634	0.11868703481576474	No Hit
GCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCC	2599	0.11710994817242693	No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA	2529	0.11395577488575133	No Hit
TAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAGG	2512	0.11318976137327298	No Hit
CCACTGGAGCCAGTGTACCCAGGGGACAATGCCACACCAGAGC	2503	0.11278422480784325	No Hit
GCCTGAGGCAGGTGCTCGCTTGGTCTAGTGCCCATTTACTCTG	2477	0.11161267472993518	No Hit
AGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCTCCCTG	2430	0.10949487266602442	No Hit
GAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACAGAAGG	2424	0.10922451495573794	No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG	2405	0.10836838220649743	No Hit
TCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTG	2364	0.10652093785287313	No Hit
GCTCTGGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGG	2362	0.10643081861611096	No Hit
CGCTTGGTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGC	2358	0.10625058014258665	No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC	2348	0.10579998395877585	No Hit
CTTCTGTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCT	2344	0.10561974548525153	No Hit
GAGCAAGCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTG	2331	0.10503397044629749	No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT	2329	0.10494385120953532	No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG	2303	0.10377230113162725	No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC	2256	0.1016544990677165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.505961838108001E-5	4.505961838108001E-5	0.0	5.8577503895404E-4	0.0
3	4.505961838108001E-5	4.505961838108001E-5	0.0	0.0013517885514324002	0.0
4	4.505961838108001E-5	4.505961838108001E-5	0.0	0.0025683982477215602	0.0
5	4.505961838108001E-5	4.505961838108001E-5	0.0	0.0030640540499134407	0.0
6	4.505961838108001E-5	4.505961838108001E-5	0.0	0.0031091136682945206	0.0
7	4.505961838108001E-5	4.505961838108001E-5	0.0	0.0036498290888674805	0.0
8	4.505961838108001E-5	4.505961838108001E-5	0.0	0.0038751271807728805	0.0
9	4.505961838108001E-5	4.505961838108001E-5	0.0	0.006488585046875521	0.0
10	1.3517885514324003E-4	4.505961838108001E-5	0.0	0.009417460241645722	0.0
11	1.8023847352432003E-4	4.505961838108001E-5	0.0	0.010453831464410562	0.0
12	1.8023847352432003E-4	4.505961838108001E-5	0.0	0.011445143068794322	0.0
13	1.8023847352432003E-4	4.505961838108001E-5	0.0	0.012121037344510522	0.0
14	1.8023847352432003E-4	4.505961838108001E-5	0.0	0.012751872001845643	0.0
15	2.2529809190540004E-4	4.505961838108001E-5	0.0	0.013923422079753722	0.0
16	2.7035771028648006E-4	9.011923676216002E-5	0.0	0.015455449104710443	0.0
17	2.7035771028648006E-4	1.3517885514324003E-4	0.0	0.01725783383995364	0.0
18	2.7035771028648006E-4	1.8023847352432003E-4	0.0	0.01842938391786172	0.0
19	3.1541732866756004E-4	1.8023847352432003E-4	0.0	0.019195397430340082	0.0
20	3.1541732866756004E-4	1.8023847352432003E-4	0.0	0.020952722547202204	0.0
21	3.1541732866756004E-4	1.8023847352432003E-4	0.0	0.023701359268448085	0.0
22	3.1541732866756004E-4	1.8023847352432003E-4	0.0	0.026945651791885843	0.0
23	3.1541732866756004E-4	2.2529809190540004E-4	0.0	0.031451613629993845	0.0
24	3.1541732866756004E-4	2.2529809190540004E-4	0.0	0.037534662111439646	0.0
25	3.1541732866756004E-4	2.2529809190540004E-4	0.0	0.039652464175350406	0.0
26	3.1541732866756004E-4	2.2529809190540004E-4	0.0	0.04379794906640977	0.0
27	3.1541732866756004E-4	2.2529809190540004E-4	0.0	0.05231421694043389	0.0
28	3.6047694704864007E-4	2.2529809190540004E-4	0.0	0.07150961437077397	0.0
29	3.6047694704864007E-4	2.2529809190540004E-4	0.0	0.10652093785287314	0.0
30	4.0553656542972004E-4	2.2529809190540004E-4	0.0	0.15022876768252075	0.0
31	4.0553656542972004E-4	2.2529809190540004E-4	0.0	0.32132013867548154	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1250	0.0	21.312	1
GCTTTAT	1555	0.0	19.868166	1
CTAGTAC	65	0.0015805601	17.076925	3
CTTTATT	1850	0.0	17.0	2
ATCTAGG	100	5.88275E-6	16.650002	1
TTGCCGA	100	5.88275E-6	16.650002	10
TTTATTG	1875	0.0	16.477335	3
CGAATTA	80	3.3843415E-4	16.1875	15
CTTATAC	920	0.0	16.086956	37
TATTGAG	1885	0.0	15.899204	5
TTAGACT	165	9.749783E-10	15.696971	4
TTATTGA	1910	0.0	15.59424	4
ACGTTCG	95	7.0644106E-5	15.578948	7
TTTCGGA	300	0.0	15.416667	30
AAGACGG	255	0.0	15.235294	5
GTTATAC	110	1.4530004E-5	15.136364	3
CGCAAGA	295	0.0	15.050847	2
TCTGTCG	125	2.9615658E-6	14.800001	8
ATATCGC	75	0.0041060066	14.8	6
TATAGAG	150	8.1114194E-8	14.8	5
>>END_MODULE