FastQCFastQC Report
Fri 10 Feb 2017
ERR1630548.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630548.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114887
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT111450.5269785099629437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106490.5035257202867104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87320.41288257954207486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63000.2978882559682858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA2200.017.6590919
AAGACGG2950.016.9322035
CGAACGA2300.016.08695616
TACGCTA1151.2433247E-616.0869569
GGTATCA50300.015.9990061
CTTATAC19400.015.73453637
ACGTTAA2500.015.54000120
ACGAACG2500.015.54000115
GACGGAC3150.015.2698427
TAGCGCA1101.4529362E-515.1363644
ATAGTAC2109.094947E-1214.976193
ATAACGA2600.014.942306512
AGTACCG1001.094054E-414.7999995
TAACGCT2650.014.66037732
TAACGAA2650.014.66037713
GTATAGG1904.5656634E-1014.6052641
AACGCTA2550.014.50980433
TGTACCG1152.211469E-514.4782615
AATTACG2950.014.42372916
CGAACTA908.278067E-414.38888824
Produced by FastQC (version 0.11.5)