##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630548.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2114887
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.898312770374964	33.0	31.0	34.0	30.0	34.0
2	32.06987891078814	33.0	31.0	34.0	30.0	34.0
3	32.17669312828534	34.0	31.0	34.0	30.0	34.0
4	35.79163756739722	37.0	35.0	37.0	35.0	37.0
5	35.742958370825484	37.0	35.0	37.0	35.0	37.0
6	35.82052279861761	37.0	35.0	37.0	35.0	37.0
7	35.767361093051306	37.0	35.0	37.0	35.0	37.0
8	35.77692094187538	37.0	35.0	37.0	35.0	37.0
9	37.44849251993133	39.0	37.0	39.0	35.0	39.0
10	37.348796413236265	39.0	37.0	39.0	34.0	39.0
11	37.44827595989762	39.0	37.0	39.0	35.0	39.0
12	37.393596442741384	39.0	37.0	39.0	34.0	39.0
13	37.44406155033342	39.0	37.0	39.0	35.0	39.0
14	38.68575389607104	40.0	38.0	41.0	35.0	41.0
15	38.693095659484406	40.0	38.0	41.0	35.0	41.0
16	38.64319512106321	40.0	38.0	41.0	34.0	41.0
17	38.64724876553688	40.0	38.0	41.0	34.0	41.0
18	38.6361181472107	40.0	38.0	41.0	34.0	41.0
19	38.69187006208843	40.0	38.0	41.0	34.0	41.0
20	38.66605591693551	40.0	38.0	41.0	34.0	41.0
21	38.619914917440035	40.0	38.0	41.0	34.0	41.0
22	38.54772098934837	40.0	38.0	41.0	34.0	41.0
23	38.48789273374889	40.0	38.0	41.0	34.0	41.0
24	38.42982863859866	40.0	38.0	41.0	34.0	41.0
25	38.37890913320665	40.0	38.0	41.0	34.0	41.0
26	38.22391078104882	40.0	38.0	41.0	34.0	41.0
27	38.079951316547884	40.0	37.0	41.0	33.0	41.0
28	37.981640626662326	40.0	37.0	41.0	33.0	41.0
29	37.89146039481069	40.0	37.0	41.0	33.0	41.0
30	37.796535228596134	40.0	37.0	41.0	33.0	41.0
31	37.73503123334722	40.0	37.0	41.0	33.0	41.0
32	37.62552183639126	40.0	37.0	41.0	33.0	41.0
33	37.52919328550414	40.0	36.0	41.0	32.0	41.0
34	37.452331968563804	40.0	36.0	41.0	32.0	41.0
35	37.38124259121173	40.0	36.0	41.0	32.0	41.0
36	37.33267498452636	40.0	36.0	41.0	32.0	41.0
37	37.18474651364352	40.0	36.0	41.0	31.0	41.0
38	37.02579854148236	39.0	36.0	41.0	31.0	41.0
39	36.94902091695679	39.0	36.0	41.0	31.0	41.0
40	36.739961520402744	39.0	35.0	41.0	30.0	41.0
41	36.67188885269047	39.0	35.0	41.0	30.0	41.0
42	36.516701365132036	39.0	35.0	41.0	30.0	41.0
43	35.72908008796688	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	10.0
11	9.0
12	3.0
13	6.0
14	6.0
15	6.0
16	10.0
17	37.0
18	67.0
19	135.0
20	314.0
21	633.0
22	1199.0
23	2067.0
24	3485.0
25	5663.0
26	8641.0
27	12795.0
28	18130.0
29	25378.0
30	34111.0
31	43329.0
32	54814.0
33	71328.0
34	93180.0
35	123825.0
36	174760.0
37	274438.0
38	507409.0
39	659089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.776476000845435	19.87382777424988	12.459294515498936	23.89040170940575
2	18.672723412645688	21.42870990270402	34.47342576695587	25.42514091769442
3	20.88745166999466	23.860045477607077	29.56484199865052	25.68766085374774
4	14.363557012738742	15.532035517736881	34.84569151921592	35.258715950308456
5	14.044249172650833	37.805140416485614	34.094823978775224	14.055786432088333
6	34.43082301796739	34.88030329752843	15.789448797973604	14.89942488653058
7	28.337211397110107	31.065300415577756	21.84126149529502	18.756226692017115
8	27.94503914393535	32.398184867560296	20.41101959584602	19.24575639265833
9	26.62392837064108	13.48596875388614	21.090914077206016	38.79918879826676
10	17.178742883189503	27.40661794223521	33.0954325219267	22.31920665264858
11	34.44165101965259	22.178442630741028	22.060422140757403	21.319484208848984
12	21.886984978393645	26.355261534067775	29.167373954258547	22.59037953328003
13	30.038106054838863	20.30548204230297	25.815374533012875	23.841037369845292
14	22.44327947545188	21.76603288970049	26.667807783583708	29.12287985126392
15	25.540087957418056	28.14713031949225	23.47042655234062	22.842355170749077
16	24.08738622914605	26.985744392017164	25.481361415527164	23.445507963309623
17	22.85086626377674	26.88923805385347	26.811645255751255	23.44825042661854
18	22.543663089328177	25.330620501237185	28.63448496302639	23.491231446408246
19	23.356756176571135	26.009947576395337	28.603845028126802	22.029451218906733
20	24.173537404126083	25.192788078039158	27.93227250439385	22.70140201344091
21	24.238127143436035	25.52803057562886	27.67372441175344	22.560117869181664
22	24.036650657931133	25.647233161866335	27.83236172901909	22.483754451183444
23	23.461395336961267	25.54297227227743	27.55873008817965	23.43690230258165
24	23.05451780638871	26.136006320905086	27.91633784689206	22.893138025814146
25	23.32100958585494	25.9672975435567	27.515654500689635	23.196038369898723
26	23.03030847511002	26.517870694746335	27.608661833941955	22.843158996201687
27	23.24105259524504	25.78974668622957	27.684126858787256	23.28507385973813
28	22.74887499899522	26.16669353965484	27.908630579317006	23.17580088203294
29	22.475479777406544	26.710741519523268	28.343121878379318	22.47065682469087
30	22.884201378135096	26.680763558525822	27.70781606771426	22.727218995624828
31	22.520352151202406	27.070193348391662	27.58658027592018	22.822874224485755
32	22.15995464533093	26.44368233385519	27.874775342606956	23.52158767820692
33	21.881358200225353	26.550543835202546	28.249168868123924	23.31892909644818
34	23.174382366528327	25.76232205314043	27.8734041109525	23.189891469378743
35	22.50087120493908	25.897412013029538	28.3275182078286	23.27419857420278
36	22.005194603777888	26.447512325717636	28.135356640803977	23.4119364297005
37	22.519832028850715	25.248393885819905	28.71557676603998	23.5161973192894
38	22.2152294661606	25.36017290758324	28.837710950986978	23.586886675269174
39	22.167425493655216	25.174914782681064	29.135504639254954	23.522155084408766
40	21.335040595549547	25.009752294094202	29.938289847164413	23.71691726319184
41	20.757941204423688	25.21302556590494	30.244594628460053	23.78443860121132
42	21.11592723393732	25.241915998348848	30.222985908939815	23.419170858774017
43	20.913552355279503	24.97239805247278	30.02628509230044	24.08776449994728
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	939.0
1	808.0
2	677.0
3	1491.0
4	2305.0
5	2305.0
6	3175.0
7	4045.0
8	3932.0
9	3819.0
10	5552.0
11	7285.0
12	7285.0
13	12184.0
14	17083.0
15	22883.5
16	28684.0
17	27989.5
18	27295.0
19	27295.0
20	31558.0
21	35821.0
22	34574.0
23	33327.0
24	37477.5
25	41628.0
26	41628.0
27	45156.5
28	48685.0
29	54581.5
30	60478.0
31	66777.0
32	73076.0
33	73076.0
34	79303.0
35	85530.0
36	92141.0
37	98752.0
38	103720.5
39	108689.0
40	108689.0
41	112190.0
42	115691.0
43	116673.0
44	117655.0
45	119182.0
46	120709.0
47	120709.0
48	125741.5
49	130774.0
50	130180.0
51	129586.0
52	129037.0
53	128488.0
54	128488.0
55	125171.5
56	121855.0
57	119657.5
58	117460.0
59	115059.5
60	112659.0
61	112659.0
62	104794.5
63	96930.0
64	88205.5
65	79481.0
66	68536.5
67	57592.0
68	57592.0
69	50335.5
70	43079.0
71	36148.0
72	29217.0
73	22552.0
74	15887.0
75	15887.0
76	11838.5
77	7790.0
78	6299.0
79	4808.0
80	3927.0
81	3046.0
82	3046.0
83	2375.0
84	1704.0
85	1412.5
86	1121.0
87	965.5
88	810.0
89	810.0
90	586.5
91	363.0
92	202.0
93	41.0
94	30.5
95	20.0
96	20.0
97	11.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2114887.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.88753163800709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.15623047698527	52.45840208978871
2	9.069952953661534	11.044940948426051
3	2.1410919726224376	3.9109741566879506
4	0.7834360024476229	1.9080593754153374
5	0.40049024054016663	1.219243109580123
6	0.24307698419229665	0.8880214539287886
7	0.16791694714363914	0.7156833902236124
8	0.1279788959203914	0.6233855259480038
9	0.0894680410746992	0.4902739363373638
>10	0.6500506413527808	7.708354301568534
>50	0.0816438424242297	3.5133603352277256
>100	0.0804771037636044	9.911042019329047
>500	0.006548718297095179	2.710118339864421
>1k	0.00132533584584069	1.1500665079472991
>5k	1.5592186421655176E-4	0.7135462996311623
>10k+	1.5592186421655176E-4	1.0345282100958988
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11145	0.5269785099629437	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10649	0.5035257202867104	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8732	0.41288257954207486	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6300	0.2978882559682858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.7283850153696154E-5	0.0
2	0.0	4.7283850153696154E-5	0.0	5.201223516906577E-4	0.0
3	0.0	4.7283850153696154E-5	0.0	0.0011820962538424038	0.0
4	0.0	4.7283850153696154E-5	0.0	0.00184407015599415	0.0
5	0.0	9.456770030739231E-5	0.0	0.0019859217064552384	0.0
6	0.0	9.456770030739231E-5	0.0	0.002080489406762631	0.0
7	0.0	9.456770030739231E-5	0.0	0.0024587602079922002	0.0
8	0.0	9.456770030739231E-5	0.0	0.0026006117584532885	0.0
9	0.0	9.456770030739231E-5	0.0	0.004066411113217869	0.0
10	0.0	9.456770030739231E-5	0.0	0.005863197419058323	0.0
11	0.0	1.8913540061478462E-4	0.0	0.006856158272285942	0.0
12	0.0	1.8913540061478462E-4	0.0	0.0075181321744376885	0.0
13	0.0	1.8913540061478462E-4	0.0	0.008274673776896828	0.0
14	0.0	1.8913540061478462E-4	0.0	0.008936647679048573	0.0
15	4.7283850153696154E-5	1.8913540061478462E-4	0.0	0.010497014734120546	4.7283850153696154E-5
16	4.7283850153696154E-5	1.8913540061478462E-4	0.0	0.013712316544571885	4.7283850153696154E-5
17	4.7283850153696154E-5	1.8913540061478462E-4	0.0	0.01796786305840454	4.7283850153696154E-5
18	4.7283850153696154E-5	1.8913540061478462E-4	0.0	0.020095636315320867	9.456770030739231E-5
19	4.7283850153696154E-5	1.8913540061478462E-4	0.0	0.0233582219759259	9.456770030739231E-5
20	4.7283850153696154E-5	1.8913540061478462E-4	0.0	0.02595883373437919	1.8913540061478462E-4
21	4.7283850153696154E-5	1.8913540061478462E-4	0.0	0.030876354150363588	1.8913540061478462E-4
22	4.7283850153696154E-5	2.3641925076848077E-4	0.0	0.03877275712603085	1.8913540061478462E-4
23	4.7283850153696154E-5	2.3641925076848077E-4	0.0	0.053809021474906224	1.8913540061478462E-4
24	4.7283850153696154E-5	2.837031009221769E-4	0.0	0.07693082420006364	1.8913540061478462E-4
25	4.7283850153696154E-5	2.837031009221769E-4	0.0	0.08307772472004414	2.3641925076848077E-4
26	4.7283850153696154E-5	2.837031009221769E-4	0.0	0.09442584875693122	2.837031009221769E-4
27	9.456770030739231E-5	3.309869510758731E-4	0.0	0.11693296143009059	2.837031009221769E-4
28	9.456770030739231E-5	4.7283850153696154E-4	0.0	0.16766853264500656	3.309869510758731E-4
29	9.456770030739231E-5	5.201223516906577E-4	0.0	0.24526133074722195	3.309869510758731E-4
30	9.456770030739231E-5	5.201223516906577E-4	0.0	0.34990049113735155	3.7827080122956923E-4
31	9.456770030739231E-5	5.201223516906577E-4	0.0	0.729542524021378	4.255546513832654E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTTA	220	0.0	17.65909	19
AAGACGG	295	0.0	16.932203	5
CGAACGA	230	0.0	16.086956	16
TACGCTA	115	1.2433247E-6	16.086956	9
GGTATCA	5030	0.0	15.999006	1
CTTATAC	1940	0.0	15.734536	37
ACGTTAA	250	0.0	15.540001	20
ACGAACG	250	0.0	15.540001	15
GACGGAC	315	0.0	15.269842	7
TAGCGCA	110	1.4529362E-5	15.136364	4
ATAGTAC	210	9.094947E-12	14.97619	3
ATAACGA	260	0.0	14.9423065	12
AGTACCG	100	1.094054E-4	14.799999	5
TAACGCT	265	0.0	14.660377	32
TAACGAA	265	0.0	14.660377	13
GTATAGG	190	4.5656634E-10	14.605264	1
AACGCTA	255	0.0	14.509804	33
TGTACCG	115	2.211469E-5	14.478261	5
AATTACG	295	0.0	14.423729	16
CGAACTA	90	8.278067E-4	14.388888	24
>>END_MODULE