FastQCFastQC Report
Fri 10 Feb 2017
ERR1630547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391734
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160924.107889537288058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122843.1358013345790767No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT106662.722765958533086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82772.112913354470125No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT79452.028161967049069RNA PCR Primer, Index 29 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG55421.4147355092996778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53901.37593366927558No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC46411.1847324970515707No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC33980.8674253447492432RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC16640.4247780381585463RNA PCR Primer, Index 29 (95% over 24bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.32879453915156714No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12130.30964889440283455No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12100.3088830686128853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10380.2649757233224586No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA9990.25501998805311765No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG9650.24634062910035892No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.2297477369847907No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.22923718645815783No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA8890.2269397090883099No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA7110.18150071221798464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6250.15954703957277133No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6170.15750483746623983No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5750.1467832764069496No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.13861446798082372No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA5340.13631699061097582No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5330.13606171534765937No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA5270.13453006376776078No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.13376423797781148No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5230.13350896271449503No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4980.1271270811315842No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4890.12482960376173628No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4740.12100047481198976No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA4640.11844772217882542No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA4630.118192446915509No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA4510.11512914375571177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4170.10644978480295303No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC4110.10491813322305442No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC4080.10415230743310512No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT4030.10287593111652293No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC4020.10262065585320652No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA3960.1010890042733079No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA3940.10057845374667504No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA3920.10006790322004218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT251.2321403E-437.04
CGTCTTC2200.032.79545637
TAGGCAT303.5951848E-430.8333345
ACATCTA303.5951848E-430.83333430
CTTGTAC250.005491975729.63
TTTAGAG250.005491975729.62
TACTGCG250.005491975729.65
CCGTCTT9900.028.96464737
GCCGTCT10150.028.25123236
TATACAC16150.027.2631593
GTTATAC358.858918E-426.428573
TTATACA16700.026.254492
TATTGAC601.3336485E-624.66666830
CTTATAC18550.023.6361181
TTTGGAG400.001928927123.1250022
ATGCCCC802.7141141E-823.12500237
TGAGATG400.001928927123.1250025
CTTTGCG603.718218E-521.5833349
CGGGAAC603.718218E-521.5833344
GAGCTGA1301.8189894E-1221.3461536
Produced by FastQC (version 0.11.5)