##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630547.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391734
Sequences flagged as poor quality	0
Sequence length	43
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.537379956807424	31.0	31.0	34.0	30.0	34.0
2	31.60807078272501	31.0	31.0	34.0	30.0	34.0
3	31.58134346265578	31.0	31.0	34.0	28.0	34.0
4	35.38331622989069	37.0	35.0	37.0	33.0	37.0
5	35.390249506042366	37.0	35.0	37.0	33.0	37.0
6	35.52281395028259	37.0	35.0	37.0	33.0	37.0
7	35.4063777971787	37.0	35.0	37.0	33.0	37.0
8	35.4939423180015	37.0	35.0	37.0	33.0	37.0
9	37.010800696390916	39.0	37.0	39.0	33.0	39.0
10	36.89847447502642	39.0	37.0	39.0	33.0	39.0
11	37.10175271995793	39.0	37.0	39.0	33.0	39.0
12	37.035210117069234	39.0	37.0	39.0	33.0	39.0
13	37.13154844869222	39.0	37.0	39.0	33.0	39.0
14	37.971391301240125	40.0	37.0	41.0	33.0	41.0
15	37.94703804111974	40.0	37.0	41.0	33.0	41.0
16	37.80071426018676	39.0	37.0	41.0	33.0	41.0
17	37.94574634828736	39.0	37.0	41.0	33.0	41.0
18	38.1298993704912	40.0	37.0	41.0	33.0	41.0
19	38.27473234388641	40.0	38.0	41.0	34.0	41.0
20	38.30014244359693	40.0	38.0	41.0	34.0	41.0
21	38.277491869482866	40.0	37.0	41.0	34.0	41.0
22	38.08986965645055	40.0	37.0	41.0	33.0	41.0
23	38.01254933194463	40.0	37.0	41.0	33.0	41.0
24	37.87646974732854	40.0	37.0	41.0	33.0	41.0
25	37.76622657211271	40.0	36.0	41.0	33.0	41.0
26	37.438994317572636	39.0	36.0	41.0	33.0	41.0
27	37.12041078895373	39.0	35.0	41.0	32.0	41.0
28	36.96787616086426	39.0	35.0	40.0	32.0	41.0
29	36.716481081550235	39.0	35.0	40.0	31.0	41.0
30	36.40794518729546	38.0	35.0	40.0	31.0	41.0
31	36.02578280159496	38.0	35.0	40.0	30.0	41.0
32	35.638246871601645	37.0	35.0	40.0	30.0	41.0
33	35.273443203806664	37.0	35.0	40.0	29.0	41.0
34	34.994981288323196	37.0	34.0	40.0	27.0	41.0
35	34.6937717941256	37.0	34.0	40.0	25.0	41.0
36	34.35868216698066	36.0	33.0	40.0	23.0	41.0
37	33.881519092036946	35.0	33.0	40.0	21.0	41.0
38	33.41883523002854	35.0	33.0	40.0	18.0	41.0
39	33.00028335554228	35.0	33.0	40.0	15.0	41.0
40	32.35991259374984	35.0	32.0	40.0	12.0	41.0
41	31.939323622662318	35.0	31.0	39.0	10.0	41.0
42	31.352034799123896	35.0	30.0	39.0	8.0	41.0
43	30.378131589292735	35.0	27.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	11.0
11	10.0
12	5.0
13	6.0
14	4.0
15	5.0
16	5.0
17	18.0
18	28.0
19	74.0
20	200.0
21	337.0
22	658.0
23	1129.0
24	1699.0
25	2644.0
26	3907.0
27	5602.0
28	7998.0
29	10132.0
30	12251.0
31	14284.0
32	16956.0
33	20667.0
34	24828.0
35	31199.0
36	44797.0
37	70043.0
38	74618.0
39	47613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.51140825151761	18.346888449815435	16.21967968059959	18.922023618067364
2	15.651181669193893	25.095090035585372	34.71666998524509	24.537058309975645
3	21.30246544849311	29.6420530257777	33.558741390841746	15.49674013488745
4	11.337284994409472	18.79106740798603	25.91197087819796	43.95967671940654
5	10.034104775179076	45.61309459990708	30.02343426917245	14.329366355741396
6	28.541816640883866	30.17915217979547	20.174659335161106	21.10437184415956
7	22.75013146676061	35.72883640429475	19.835143234950248	21.68588889399439
8	36.87144848289911	27.805602781479273	20.086589369316936	15.236359366304686
9	26.810795080335126	9.45258772534424	20.817952998718518	42.91866419560212
10	18.929681875966857	33.35043677597553	24.499788121531445	23.220093226526163
11	27.49825136444628	24.013744020176958	19.8126790117784	28.675325603598363
12	18.677725191073534	34.615325705708464	26.314539968447974	20.392409134770023
13	33.64732190721254	17.08251007060914	30.073212945519153	19.19695507665916
14	22.992898242174537	23.32501135974922	31.170386027253187	22.511704370823058
15	28.14843746011324	25.35240750100834	26.577218214400588	19.921936824477836
16	22.921421168445935	23.097561100134275	30.884477732338784	23.09653999908101
17	15.104126779906773	34.85171059953948	30.599079987951004	19.445082632602738
18	18.49111897358922	20.51213323326543	35.90497633598309	25.09177145716226
19	17.473336498746598	28.027692260564567	38.66271500559053	15.836256235098306
20	19.674064543797577	22.00038801840024	38.59379068449509	19.731756753307092
21	23.007448932183575	21.658319165556218	36.010915570259414	19.323316332000797
22	19.81701869125478	29.776583089545454	33.77113040992102	16.635267809278744
23	19.83054828021055	24.3670449846069	34.274788504444345	21.527618230738206
24	17.846548933715226	27.520205037091493	37.567073575436396	17.066172453756888
25	16.63067285453905	28.323301015484997	37.02308198930907	18.02294414066688
26	19.12624382872051	27.358360520148878	36.22100711196883	17.29438853916178
27	16.301112489597532	31.931616862462793	35.843199722260515	15.92407092567916
28	15.53247867175175	26.954259778318963	38.12536057630943	19.387900973619853
29	13.925520889174797	29.431450933541637	39.711896337821074	16.931131839462495
30	17.548642701424946	28.315132207058873	40.25563264868507	13.880592442831105
31	13.598513276866445	29.94072508385792	34.880811979557556	21.57994965971807
32	14.139186284570652	33.16280945743795	38.674968218229715	14.023036039761674
33	15.988655567298219	30.091848039741254	38.826091174113046	15.093405218847483
34	14.438368893177515	32.59175869339909	33.924295567910875	19.045576845512517
35	13.553584830522752	29.828659243262006	41.42760138257083	15.190154543644413
36	14.736530400731107	33.21080120694144	32.95016516309537	19.10250322923208
37	14.079962423481241	32.62571030342018	35.343115481423624	17.95121179167496
38	14.487637018997585	28.624525826198386	37.092772136194455	19.795065018609566
39	17.142244482225184	29.716338127402782	34.1328554580404	19.008561932331634
40	13.516569917341869	28.715914370465672	39.97431930851037	17.793196403682092
41	14.204792027242977	31.171407128306456	33.103585596348545	21.52021524810203
42	15.03698938565455	29.219827740252313	37.09481433830099	18.648368535792144
43	13.306988926159077	30.502585938417397	36.485982835291296	19.704442300132232
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	61.5
2	112.0
3	636.0
4	1160.0
5	1160.0
6	1963.0
7	2766.0
8	3217.0
9	3668.0
10	4931.5
11	6195.0
12	6195.0
13	10321.0
14	14447.0
15	23185.5
16	31924.0
17	27150.0
18	22376.0
19	22376.0
20	23930.5
21	25485.0
22	18077.0
23	10669.0
24	9688.5
25	8708.0
26	8708.0
27	9059.0
28	9410.0
29	9372.5
30	9335.0
31	9291.5
32	9248.0
33	9248.0
34	8520.5
35	7793.0
36	7573.0
37	7353.0
38	8225.0
39	9097.0
40	9097.0
41	10286.0
42	11475.0
43	12946.0
44	14417.0
45	17405.0
46	20393.0
47	20393.0
48	23495.0
49	26597.0
50	32158.5
51	37720.0
52	25751.0
53	13782.0
54	13782.0
55	18800.5
56	23819.0
57	20020.5
58	16222.0
59	12726.5
60	9231.0
61	9231.0
62	8941.0
63	8651.0
64	8338.5
65	8026.0
66	7389.5
67	6753.0
68	6753.0
69	6062.0
70	5371.0
71	4622.0
72	3873.0
73	3224.0
74	2575.0
75	2575.0
76	2024.5
77	1474.0
78	1178.5
79	883.0
80	654.0
81	425.0
82	425.0
83	308.0
84	191.0
85	127.5
86	64.0
87	42.0
88	20.0
89	20.0
90	13.5
91	7.0
92	6.5
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	391734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.914763222394726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02254918752601	33.15018415682457
2	10.306440190249946	8.433711200996989
3	3.234672951349345	3.9703763371902947
4	1.5598586053009713	2.5528498198521645
5	0.8478741061910797	1.73452841486038
6	0.5505822449158648	1.3516165311112291
7	0.38504695612791573	1.1027873527634238
8	0.2837691234996193	0.928827719825073
9	0.20236580131279422	0.7451773960520837
>10	1.3690075559205654	11.029122083280395
>50	0.1211014589384278	3.5452628655798306
>100	0.09862902325913192	8.210983060696345
>500	0.009363514866373284	2.6467516083858356
>1k	0.004369640270974199	3.6910985471766953
>5k	0.0024969372976995424	6.935240101718515
>10k+	0.0018727029732746568	9.97148280368617
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16092	4.107889537288058	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12284	3.1358013345790767	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	10666	2.722765958533086	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8277	2.112913354470125	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	7945	2.028161967049069	RNA PCR Primer, Index 29 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	5542	1.4147355092996778	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5390	1.37593366927558	No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	4641	1.1847324970515707	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	3398	0.8674253447492432	RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	1664	0.4247780381585463	RNA PCR Primer, Index 29 (95% over 24bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1288	0.32879453915156714	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1213	0.30964889440283455	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1210	0.3088830686128853	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1038	0.2649757233224586	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	999	0.25501998805311765	No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	965	0.24634062910035892	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	900	0.2297477369847907	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	898	0.22923718645815783	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	889	0.2269397090883099	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	711	0.18150071221798464	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	625	0.15954703957277133	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	617	0.15750483746623983	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	575	0.1467832764069496	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	543	0.13861446798082372	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	534	0.13631699061097582	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	533	0.13606171534765937	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	527	0.13453006376776078	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	524	0.13376423797781148	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	523	0.13350896271449503	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	498	0.1271270811315842	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	489	0.12482960376173628	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	474	0.12100047481198976	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	464	0.11844772217882542	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	463	0.118192446915509	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	451	0.11512914375571177	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	417	0.10644978480295303	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	411	0.10491813322305442	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	408	0.10415230743310512	No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT	403	0.10287593111652293	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	402	0.10262065585320652	No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA	396	0.1010890042733079	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	394	0.10057845374667504	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	392	0.10006790322004218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0010211010532657364	0.0
3	0.0	0.0	0.0	0.0033185784231136435	0.0
4	0.0	0.0	0.0	0.006126606319594418	0.0
5	0.0	0.0	0.0	0.007147707372860155	0.0
6	0.0	0.0	0.0	0.007147707372860155	0.0
7	0.0	0.0	0.0	0.0074029826361765895	0.0
8	0.0	0.0	0.0	0.007658257899493023	0.0
9	0.0	0.0	0.0	0.009700460006024496	0.0
10	2.552752633164341E-4	2.552752633164341E-4	0.0	0.012253212639188837	0.0
11	2.552752633164341E-4	2.552752633164341E-4	0.0	0.014805965272353179	0.0
12	5.105505266328682E-4	2.552752633164341E-4	0.0	0.019400920012048992	0.0
13	5.105505266328682E-4	2.552752633164341E-4	0.0	0.020932571591947596	0.0
14	5.105505266328682E-4	2.552752633164341E-4	0.0	0.02527225106832698	0.0
15	5.105505266328682E-4	2.552752633164341E-4	0.0	0.030633031597972094	0.0
16	5.105505266328682E-4	2.552752633164341E-4	0.0	0.04773647424017318	0.0
17	5.105505266328682E-4	2.552752633164341E-4	0.0	0.07836950583814527	0.0
18	5.105505266328682E-4	2.552752633164341E-4	0.0	0.08730414005422046	0.0
19	5.105505266328682E-4	2.552752633164341E-4	0.0	0.10644978480295303	0.0
20	5.105505266328682E-4	2.552752633164341E-4	0.0	0.12023464902204047	0.0
21	5.105505266328682E-4	2.552752633164341E-4	0.0	0.15290988272654404	0.0
22	5.105505266328682E-4	2.552752633164341E-4	0.0	0.2192814511888169	0.0
23	5.105505266328682E-4	2.552752633164341E-4	0.0	0.3382397238942752	0.0
24	5.105505266328682E-4	2.552752633164341E-4	0.0	0.5424599345474225	0.0
25	5.105505266328682E-4	2.552752633164341E-4	0.0	0.5825381508881027	0.0
26	5.105505266328682E-4	2.552752633164341E-4	0.0	0.6494202698770084	0.0
27	5.105505266328682E-4	2.552752633164341E-4	0.0	0.6969014688538652	0.0
28	5.105505266328682E-4	2.552752633164341E-4	0.0	0.7642941383694037	0.0
29	5.105505266328682E-4	2.552752633164341E-4	0.0	0.8753388779120526	0.0
30	5.105505266328682E-4	2.552752633164341E-4	0.0	1.1010022106837802	0.0
31	5.105505266328682E-4	2.552752633164341E-4	0.0	1.7297451842321576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTACT	25	1.2321403E-4	37.0	4
CGTCTTC	220	0.0	32.795456	37
TAGGCAT	30	3.5951848E-4	30.833334	5
ACATCTA	30	3.5951848E-4	30.833334	30
CTTGTAC	25	0.0054919757	29.6	3
TTTAGAG	25	0.0054919757	29.6	2
TACTGCG	25	0.0054919757	29.6	5
CCGTCTT	990	0.0	28.964647	37
GCCGTCT	1015	0.0	28.251232	36
TATACAC	1615	0.0	27.263159	3
GTTATAC	35	8.858918E-4	26.42857	3
TTATACA	1670	0.0	26.25449	2
TATTGAC	60	1.3336485E-6	24.666668	30
CTTATAC	1855	0.0	23.636118	1
TTTGGAG	40	0.0019289271	23.125002	2
ATGCCCC	80	2.7141141E-8	23.125002	37
TGAGATG	40	0.0019289271	23.125002	5
CTTTGCG	60	3.718218E-5	21.583334	9
CGGGAAC	60	3.718218E-5	21.583334	4
GAGCTGA	130	1.8189894E-12	21.346153	6
>>END_MODULE