FastQCFastQC Report
Fri 10 Feb 2017
ERR1630546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211959
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT109380.49449379486690304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98100.4434982746063557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83720.3784880280330693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54150.2448056225273615No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC25540.11546326129914704No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA23900.1080490189917625No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA22890.103482930741483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.018.2225021
TCTATAC2400.016.9583323
TCGGTGA3600.016.44444513
CGCAATA1708.54925E-1116.3235336
GCTTTAT8500.016.3235281
TCTACGA803.3843346E-416.18752
TCTTATA31150.015.32263337
CTTATAC19450.015.21850937
CGGTGAT3900.015.17948714
TATTACG750.004106001514.82
CGAATTA1406.0015736E-714.53571415
TAATTCG908.2782796E-414.3888885
TACGACG2500.014.0600015
CAGTCGG4250.013.92941110
TTCGATC800.006301049613.8753
ACCTAGG10150.013.8522171
GTCCTAA4550.013.8241751
GTATCTA9150.013.74863426
TAACGGC950.001245710613.63157936
CTAGCGG1652.5980444E-713.45454629
Produced by FastQC (version 0.11.5)