##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630546.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2211959
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899879699397683	33.0	31.0	34.0	30.0	34.0
2	32.07377939645355	33.0	31.0	34.0	30.0	34.0
3	32.1787004189499	34.0	31.0	34.0	30.0	34.0
4	35.791056705843104	37.0	35.0	37.0	35.0	37.0
5	35.74720733973821	37.0	35.0	37.0	35.0	37.0
6	35.823869701020676	37.0	35.0	37.0	35.0	37.0
7	35.774089845245776	37.0	35.0	37.0	35.0	37.0
8	35.77646782783948	37.0	35.0	37.0	35.0	37.0
9	37.4573186031025	39.0	37.0	39.0	35.0	39.0
10	37.350463096287044	39.0	37.0	39.0	34.0	39.0
11	37.452443286697445	39.0	37.0	39.0	35.0	39.0
12	37.39186983122201	39.0	37.0	39.0	34.0	39.0
13	37.44344990119618	39.0	37.0	39.0	35.0	39.0
14	38.69198524927451	40.0	38.0	41.0	35.0	41.0
15	38.697857871687496	40.0	38.0	41.0	35.0	41.0
16	38.65543936393034	40.0	38.0	41.0	34.0	41.0
17	38.64773623742574	40.0	38.0	41.0	34.0	41.0
18	38.64403770594301	40.0	38.0	41.0	34.0	41.0
19	38.69815172885212	40.0	38.0	41.0	34.0	41.0
20	38.674104718939184	40.0	38.0	41.0	34.0	41.0
21	38.62421274535378	40.0	38.0	41.0	34.0	41.0
22	38.553125532616114	40.0	38.0	41.0	34.0	41.0
23	38.49922489521732	40.0	38.0	41.0	34.0	41.0
24	38.44495218943931	40.0	38.0	41.0	34.0	41.0
25	38.39586221986936	40.0	38.0	41.0	34.0	41.0
26	38.24105962181035	40.0	38.0	41.0	34.0	41.0
27	38.10834016362871	40.0	38.0	41.0	33.0	41.0
28	38.013888593775924	40.0	37.0	41.0	33.0	41.0
29	37.94114538289362	40.0	37.0	41.0	33.0	41.0
30	37.85053294387464	40.0	37.0	41.0	33.0	41.0
31	37.787705377902576	40.0	37.0	41.0	33.0	41.0
32	37.69044679399573	40.0	37.0	41.0	33.0	41.0
33	37.59497667000157	40.0	37.0	41.0	33.0	41.0
34	37.52565395651547	40.0	37.0	41.0	32.0	41.0
35	37.462517162388636	40.0	37.0	41.0	32.0	41.0
36	37.424670620025054	40.0	36.0	41.0	32.0	41.0
37	37.287690685044346	40.0	36.0	41.0	32.0	41.0
38	37.14287380552714	40.0	36.0	41.0	31.0	41.0
39	37.0742441428616	39.0	36.0	41.0	31.0	41.0
40	36.87964424295387	39.0	35.0	41.0	31.0	41.0
41	36.8174911921966	39.0	35.0	41.0	31.0	41.0
42	36.67445734753673	39.0	35.0	41.0	30.0	41.0
43	35.88395218898723	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	9.0
11	9.0
12	7.0
13	4.0
14	4.0
15	2.0
16	9.0
17	24.0
18	57.0
19	138.0
20	276.0
21	603.0
22	1123.0
23	1877.0
24	3374.0
25	5452.0
26	8596.0
27	12678.0
28	18688.0
29	25201.0
30	34307.0
31	44497.0
32	56847.0
33	73451.0
34	96171.0
35	128608.0
36	181120.0
37	286913.0
38	538066.0
39	693837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.09466902415461	19.565914196420458	12.256330248435889	24.083086530989046
2	18.8629626498502	21.16883721624135	34.58608410011216	25.382116033796287
3	20.782980154695455	23.634976959337852	29.258363287927125	26.32367959803957
4	14.345699897692498	15.216828160015625	34.88242774843476	35.55504419385712
5	14.016670290905031	37.716567079227055	34.243989151697654	14.022773478170256
6	34.707243669525525	34.97130823853426	15.548072997736394	14.773375094203825
7	29.03860333758447	30.66141822701054	21.41088510230072	18.889093333104277
8	27.989578468678666	32.57438315990486	20.046122012207277	19.389916359209188
9	26.75759360819979	13.354768329792732	20.435595777317754	39.452042284689725
10	17.20226279058518	27.149373021832684	32.84025608069589	22.808108106886248
11	35.03921184795921	22.016863784545734	21.80709497780022	21.136829389694835
12	22.38825403183332	26.062191930320587	28.815091057293557	22.734462980552532
13	30.504905380253433	19.98341741415641	25.304221280774193	24.207455924815964
14	22.74608164075374	21.144469675975007	26.255685571025506	29.85376311224575
15	25.74166157690988	28.00164921682545	23.028772233120055	23.22791697314462
16	24.55474988460455	26.69330670233942	24.80140906770876	23.95053434534727
17	23.17095389200252	26.6994550983992	26.177157894879606	23.952433114718673
18	22.89938466309728	25.190882832819234	27.9888099191712	23.920922584912287
19	23.883715746991694	25.521223494648858	28.013313085821213	22.581747672538235
20	24.7184057209017	24.894267931729296	27.236897248095467	23.150429099273538
21	24.697112378665246	25.14305192817769	27.0058350991135	23.154000594043563
22	24.54597937846045	25.395904716136243	27.17532286990853	22.88279303549478
23	24.012470393890663	25.30607484135104	26.84489179048979	23.83656297426851
24	23.514314686664626	25.796273800735005	27.28536107586081	23.40405043673956
25	23.751977319651946	25.731399180545388	26.89805733288908	23.61856616691358
26	23.464720639035352	26.149354486226915	27.06180358677534	23.32412128796239
27	23.683124325541296	25.407613793926558	27.1285317675418	23.78073011299034
28	23.236009347370363	25.741706785704437	27.33549762902477	23.68678623790043
29	23.161143583583602	26.345967533756276	27.627184771507974	22.86570411115215
30	23.45793931985177	26.277295374823854	26.882189045999493	23.382576259324882
31	22.953951678127847	26.777033389859394	27.08870281953689	23.180312112475864
32	22.584460200211666	26.061106919251216	27.179120408651336	24.175312471885782
33	22.346797567224346	26.256002032587407	27.654807344982434	23.742393055205817
34	23.725123295684956	25.1496976209776	27.497209487155956	23.627969596181483
35	23.035101464358064	25.457117423966718	27.616605913581584	23.891175198093638
36	22.440922277492483	26.22349690930076	27.50787876267146	23.827702050535297
37	22.948164952424523	24.95801233205498	28.113450565765458	23.980372149755034
38	22.667373129429613	25.197438108030028	28.130810742875433	24.00437801966492
39	22.6610438981916	24.81375106862288	28.550619609133804	23.97458542405171
40	21.851851684411873	24.715241105282693	29.181734381152637	24.2511728291528
41	21.243341309671653	24.932424154335592	29.550592935945012	24.27364160004774
42	21.50708941711849	24.863661577814057	29.751636445340985	23.87761255972647
43	21.458851633325935	24.542181839717646	29.467499171548837	24.53146735540758
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	609.0
1	547.0
2	485.0
3	1222.5
4	1960.0
5	1960.0
6	2679.0
7	3398.0
8	3349.5
9	3301.0
10	4712.5
11	6124.0
12	6124.0
13	10308.5
14	14493.0
15	20103.5
16	25714.0
17	25036.5
18	24359.0
19	24359.0
20	27561.5
21	30764.0
22	29082.0
23	27400.0
24	31364.5
25	35329.0
26	35329.0
27	39886.0
28	44443.0
29	50191.0
30	55939.0
31	62488.5
32	69038.0
33	69038.0
34	76562.5
35	84087.0
36	91000.0
37	97913.0
38	105087.0
39	112261.0
40	112261.0
41	116952.5
42	121644.0
43	124482.5
44	127321.0
45	130324.0
46	133327.0
47	133327.0
48	139653.5
49	145980.0
50	147602.0
51	149224.0
52	147999.5
53	146775.0
54	146775.0
55	144249.0
56	141723.0
57	137279.0
58	132835.0
59	129212.5
60	125590.0
61	125590.0
62	114619.5
63	103649.0
64	93298.0
65	82947.0
66	71174.5
67	59402.0
68	59402.0
69	51518.5
70	43635.0
71	36509.0
72	29383.0
73	21913.5
74	14444.0
75	14444.0
76	10572.5
77	6701.0
78	5350.0
79	3999.0
80	3270.5
81	2542.0
82	2542.0
83	1954.0
84	1366.0
85	1113.5
86	861.0
87	753.0
88	645.0
89	645.0
90	464.5
91	284.0
92	166.0
93	48.0
94	32.0
95	16.0
96	16.0
97	8.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2211959.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.46097998427158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.02060503125642	52.869106840609824
2	9.15522340314873	11.253780046649178
3	2.092925141727027	3.858996907327908
4	0.8174512456387655	2.009654185852882
5	0.41557368802841216	1.277078306095208
6	0.2561100846525725	0.9444466071961118
7	0.18182834728294864	0.7822743889051341
8	0.1333043916612106	0.6554414838164132
9	0.09595244212344095	0.5307598012311705
>10	0.6683328204726603	7.973766966211427
>50	0.08263055445009995	3.5594829736015186
>100	0.07292774465950017	9.076644127999817
>500	0.005075486989342795	2.134242237162416
>1k	0.0017653867789018183	1.5130207793406596
>5k	2.206733473627273E-4	1.0668046532435398
>10k+	7.355778245424243E-5	0.49449969475686906
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10938	0.49449379486690304	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9810	0.4434982746063557	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8372	0.3784880280330693	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5415	0.2448056225273615	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2554	0.11546326129914704	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2390	0.1080490189917625	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2289	0.103482930741483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3562638367166843E-4	0.0
2	0.0	0.0	0.0	0.0010850110693733474	0.0
3	0.0	0.0	0.0	0.0017631429877316895	0.0
4	0.0	0.0	0.0	0.003074198029891151	0.0
5	0.0	0.0	0.0	0.0033454507972344874	0.0
6	0.0	0.0	0.0	0.0033906595917917105	0.0
7	0.0	0.0	0.0	0.004204417893821721	0.0
8	0.0	0.0	0.0	0.004746923428508395	0.0
9	0.0	0.0	0.0	0.008318418198528996	0.0
10	0.0	0.0	0.0	0.012342000914121826	4.52087945572228E-5
11	0.0	0.0	0.0	0.013788682339952956	4.52087945572228E-5
12	0.0	0.0	0.0	0.015054528587555195	4.52087945572228E-5
13	0.0	0.0	0.0	0.015642242916799092	4.52087945572228E-5
14	0.0	0.0	0.0	0.01659162760250077	4.52087945572228E-5
15	0.0	0.0	0.0	0.018083517822889122	9.04175891144456E-5
16	0.0	0.0	0.0	0.020524792728979155	9.04175891144456E-5
17	0.0	0.0	0.0	0.02450316665001476	9.04175891144456E-5
18	0.0	0.0	0.0	0.02608547445951756	9.04175891144456E-5
19	0.0	0.0	0.0	0.028617166954722037	9.04175891144456E-5
20	0.0	0.0	0.0	0.031013233066254843	9.04175891144456E-5
21	0.0	0.0	0.0	0.036302662029449914	9.04175891144456E-5
22	0.0	0.0	0.0	0.045073168173551136	9.04175891144456E-5
23	0.0	0.0	0.0	0.058274136184260195	9.04175891144456E-5
24	0.0	0.0	0.0	0.07703578592550767	9.04175891144456E-5
25	0.0	0.0	0.0	0.08237042368325995	9.04175891144456E-5
26	0.0	0.0	0.0	0.09123134741647562	9.04175891144456E-5
27	0.0	0.0	0.0	0.10691879912783193	9.04175891144456E-5
28	0.0	0.0	0.0	0.14638607677628745	9.04175891144456E-5
29	0.0	0.0	0.0	0.21166757611691717	9.04175891144456E-5
30	0.0	0.0	0.0	0.30841439646937396	9.04175891144456E-5
31	0.0	0.0	0.0	0.6704464232836143	9.04175891144456E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4000	0.0	18.222502	1
TCTATAC	240	0.0	16.958332	3
TCGGTGA	360	0.0	16.444445	13
CGCAATA	170	8.54925E-11	16.32353	36
GCTTTAT	850	0.0	16.323528	1
TCTACGA	80	3.3843346E-4	16.1875	2
TCTTATA	3115	0.0	15.322633	37
CTTATAC	1945	0.0	15.218509	37
CGGTGAT	390	0.0	15.179487	14
TATTACG	75	0.0041060015	14.8	2
CGAATTA	140	6.0015736E-7	14.535714	15
TAATTCG	90	8.2782796E-4	14.388888	5
TACGACG	250	0.0	14.060001	5
CAGTCGG	425	0.0	13.929411	10
TTCGATC	80	0.0063010496	13.875	3
ACCTAGG	1015	0.0	13.852217	1
GTCCTAA	455	0.0	13.824175	1
GTATCTA	915	0.0	13.748634	26
TAACGGC	95	0.0012457106	13.631579	36
CTAGCGG	165	2.5980444E-7	13.454546	29
>>END_MODULE