FastQCFastQC Report
Fri 10 Feb 2017
ERR1630545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513293
Sequences flagged as poor quality0
Sequence length43
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT203183.958362962284699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT180603.5184582684743413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT167123.2558402316026127No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105522.0557459384795815No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32940.641738734017413No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29740.5793961733356972No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA24780.4827652042790375No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24040.4683484871213907No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20070.3910047477756369No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA19250.3750294666009472No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT18600.36236613396247364No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16630.3239864950427923No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA14610.28463275361245916No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA14310.2787881385485483No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13420.2614491138589461No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT11950.23281050004578283No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.2168352188710931No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11110.21644557786683238No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.21469219334765913No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA10130.1973531686580569No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9650.1880017845557995No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA9530.18566393853023516No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA9510.18527429752597444No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT9100.1772866569386296TruSeq Adapter, Index 6 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT8690.16929901635128475No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA8300.16170101676820062No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA7910.1541030171851165No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA7560.1472842996105538No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA7170.13968630002746968No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6610.1287763519081694No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA6420.12507476236769252No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA6350.12371101885277999No Hit
ACGCAGAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAA6340.12351619835064963No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA6290.12254209583999781No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA6140.11961978830804239No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC6120.11923014730378166No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT5900.11494409625691368No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5850.11396999374626189No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5390.10500825064826522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCAG250.005493544529.63
TACTGTG250.005493544529.67
TATGTCG250.005493544529.65
TATCGGT250.005493544529.613
AAAATGC250.005493544529.626
ACACTGC405.9338236E-527.756
TATACCA652.6780453E-622.769235
CCGTCTT1800.022.61111337
GCCGTCT1800.022.61111336
TACACTG502.6992796E-422.25
GCGTTAT603.7211168E-521.5833321
GAACAGA603.7211168E-521.5833321
AGCGTCA957.1213435E-921.4210533
ATGCCCC1151.3460522E-1020.91304437
ATACACT806.944683E-720.81254
GATACAC909.45256E-820.5555573
ACAGAGG450.003822965820.5555573
ATACAGG450.003822965820.5555571
TCTATGA450.003822965820.5555572
TATGCCG2300.019.30434833
Produced by FastQC (version 0.11.5)