##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630545.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513293
Sequences flagged as poor quality	0
Sequence length	43
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9506811898857	33.0	31.0	34.0	30.0	34.0
2	31.969847630885283	33.0	31.0	34.0	30.0	34.0
3	31.93927639769099	33.0	31.0	34.0	30.0	34.0
4	35.6477177752278	37.0	35.0	37.0	33.0	37.0
5	35.66240334467838	37.0	35.0	37.0	35.0	37.0
6	35.83654949512267	37.0	35.0	37.0	35.0	37.0
7	35.72156448656031	37.0	35.0	37.0	33.0	37.0
8	35.84863226266479	37.0	35.0	37.0	35.0	37.0
9	37.36538000713043	39.0	37.0	39.0	34.0	39.0
10	37.3364881266645	39.0	37.0	39.0	34.0	39.0
11	37.492383492469216	39.0	37.0	39.0	35.0	39.0
12	37.507119715250354	39.0	37.0	39.0	35.0	39.0
13	37.52805902281933	39.0	37.0	39.0	35.0	39.0
14	38.61250981408279	40.0	38.0	41.0	34.0	41.0
15	38.490072921313946	40.0	38.0	41.0	34.0	41.0
16	38.33735312969396	40.0	38.0	41.0	34.0	41.0
17	38.568946001601425	40.0	38.0	41.0	34.0	41.0
18	38.69183682614024	40.0	38.0	41.0	35.0	41.0
19	38.87013460148492	40.0	38.0	41.0	35.0	41.0
20	38.90729466406127	40.0	38.0	41.0	35.0	41.0
21	38.8738089161551	40.0	38.0	41.0	35.0	41.0
22	38.733010191060465	40.0	38.0	41.0	35.0	41.0
23	38.599914668620066	40.0	38.0	41.0	35.0	41.0
24	38.48829226192448	40.0	38.0	41.0	34.0	41.0
25	38.37788943157222	40.0	37.0	41.0	34.0	41.0
26	38.02306285104219	40.0	37.0	41.0	34.0	41.0
27	37.739823064019966	40.0	35.0	41.0	33.0	41.0
28	37.518787515122945	39.0	35.0	41.0	33.0	41.0
29	37.25152495748043	39.0	35.0	41.0	33.0	41.0
30	36.9044074242197	39.0	35.0	41.0	33.0	41.0
31	36.48902478701249	38.0	35.0	40.0	32.0	41.0
32	36.07811717673921	37.0	35.0	40.0	31.0	41.0
33	35.696631358697665	37.0	35.0	40.0	30.0	41.0
34	35.40067368929637	37.0	35.0	40.0	30.0	41.0
35	35.03869914454317	37.0	35.0	40.0	27.0	41.0
36	34.63267178784827	36.0	35.0	40.0	24.0	41.0
37	34.09571531269665	35.0	34.0	40.0	21.0	41.0
38	33.545033733169944	35.0	33.0	40.0	18.0	41.0
39	33.037066548735325	35.0	33.0	40.0	15.0	41.0
40	32.3881934879299	35.0	33.0	40.0	10.0	41.0
41	31.80705172289511	35.0	32.0	40.0	8.0	41.0
42	31.124289635744105	35.0	31.0	40.0	7.0	41.0
43	30.250317070367217	35.0	26.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	18.0
11	17.0
12	9.0
13	12.0
14	6.0
15	7.0
16	10.0
17	17.0
18	28.0
19	80.0
20	176.0
21	353.0
22	656.0
23	1056.0
24	1697.0
25	2751.0
26	4250.0
27	6353.0
28	8767.0
29	11917.0
30	14676.0
31	16781.0
32	19839.0
33	24313.0
34	30307.0
35	36097.0
36	50304.0
37	94352.0
38	95758.0
39	92683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.47470742831093	19.054029569855814	13.714389247466846	14.756873754366415
2	16.520778580654714	26.145690667903125	33.884740294529635	23.448790456912523
3	25.013393909521465	31.410714737976164	28.849799237472556	14.726092115029818
4	13.153306201331402	17.94978696378092	29.880984155248562	39.01592267963912
5	9.91324643040135	46.769778664427534	28.326316548248272	14.990658356922848
6	28.869281287685588	29.15527778481296	20.065732437418784	21.909708490082664
7	23.12363503885695	38.86298858546678	22.094593146604378	15.918783229071895
8	35.15574925042812	27.52424054097757	19.748564659950553	17.57144554864376
9	30.792938925720787	10.84955376363987	24.086243139883067	34.27126417075627
10	21.068668382385887	32.98564367719801	24.621609879737306	21.324078060678794
11	24.85110843124687	28.258519013506906	18.61178703001989	28.278585525226337
12	21.56877261135453	34.73201465829458	28.69628068179383	15.002932048557064
13	30.099572758638825	21.129647199552693	31.89094727572751	16.87983276608097
14	24.389968302704304	24.208395594718805	33.713493073157046	17.688143029419845
15	28.916038208196877	22.755229469328434	32.6782169248363	15.650515397638387
16	18.74095302293232	25.80767709670695	36.330516878274196	19.120853002086527
17	16.05554722156741	31.245701772671747	34.06007874644696	18.638672259313882
18	14.960071538088382	23.223383135947696	40.215432511255756	21.601112814708166
19	16.297319464711187	27.874917444812226	42.850964264075294	12.976798826401295
20	18.008817575926418	22.528068763844434	44.61779139789555	14.8453222623336
21	21.310245805027538	23.413333125524797	40.90899349883985	14.367427570607822
22	19.88493901144181	24.332691075077978	39.90274560533653	15.879624308143692
23	17.092966395411587	26.728398789775042	39.828518994024854	16.350115820788517
24	15.075210454847426	28.599065251230776	41.20745850810356	15.118265785818236
25	15.145540656116488	27.76620760462348	40.61851613016347	16.46973560909656
26	16.498763863913982	27.215060404096686	40.737356636463	15.548819095526337
27	14.739924370681074	29.28950911078078	40.194391897025675	15.776174621512471
28	14.394897261408202	28.979549691891375	42.02414605303404	14.601406993666386
29	13.89946872449069	30.306666952403404	41.90764339276008	13.886220930345825
30	13.272925989639445	31.503838937994477	42.13986943129947	13.083365641066603
31	12.85055514102082	32.4709279105696	40.24153845854122	14.43697848986836
32	11.793848737465735	33.31177319776424	41.16518245914127	13.729195605628755
33	12.192256664322326	32.785563021510136	41.02783400513936	13.994346309028177
34	12.866335601693379	34.179893355257136	38.32937523013172	14.624395812917768
35	12.531049517527027	34.43218590551596	38.18403913554247	14.852725441414552
36	12.142577436279083	34.90735311021191	37.36072769353956	15.589341759969452
37	12.394480345533642	35.38388405842277	37.0587559152375	15.16287968080609
38	12.643460947256246	33.996762083254595	37.55963942621466	15.800137543274504
39	13.347542241955374	34.57869092311798	37.20526093283953	14.868505902087112
40	12.273301993208557	34.90384634117356	36.99797191857282	15.82487974704506
41	11.616562080527107	36.16004893891013	36.11504540291802	16.10834357764474
42	11.879180117398834	35.65702240240954	36.38350805485366	16.080289425337966
43	11.390375477553755	36.927252076299496	35.219845195629006	16.462527250517738
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26.0
1	140.5
2	255.0
3	1295.0
4	2335.0
5	2335.0
6	3931.5
7	5528.0
8	5753.0
9	5978.0
10	8019.0
11	10060.0
12	10060.0
13	17760.5
14	25461.0
15	35392.5
16	45324.0
17	40976.0
18	36628.0
19	36628.0
20	37620.0
21	38612.0
22	26892.5
23	15173.0
24	14099.5
25	13026.0
26	13026.0
27	13604.0
28	14182.0
29	14437.5
30	14693.0
31	14739.0
32	14785.0
33	14785.0
34	14497.5
35	14210.0
36	14254.0
37	14298.0
38	15893.0
39	17488.0
40	17488.0
41	19171.5
42	20855.0
43	22268.5
44	23682.0
45	26757.0
46	29832.0
47	29832.0
48	33007.0
49	36182.0
50	33136.0
51	30090.0
52	22565.0
53	15040.0
54	15040.0
55	13944.5
56	12849.0
57	11782.0
58	10715.0
59	9838.5
60	8962.0
61	8962.0
62	8628.0
63	8294.0
64	7710.5
65	7127.0
66	6661.0
67	6195.0
68	6195.0
69	5647.5
70	5100.0
71	4491.0
72	3882.0
73	3323.5
74	2765.0
75	2765.0
76	2310.0
77	1855.0
78	1383.0
79	911.0
80	720.0
81	529.0
82	529.0
83	377.5
84	226.0
85	164.0
86	102.0
87	64.5
88	27.0
89	27.0
90	17.0
91	7.0
92	5.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	513293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.590211411123406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58535919190605	26.83693976855977
2	11.15432583223075	7.716609773708334
3	3.840148734245031	3.984946697030807
4	1.8396601711906955	2.5453693698443858
5	1.1422859980149145	1.975595708165099
6	0.7364045289451154	1.5283433004192173
7	0.5555118912812751	1.3450691632568617
8	0.3631312907240103	1.0048630492910107
9	0.28144222905960925	0.8761631582870734
>10	2.083460499031862	13.912102720176184
>50	0.24239345330107584	5.795208484862069
>100	0.1527642461502129	11.244510040208128
>500	0.011837819812378119	2.902576410809083
>1k	0.009019291285621425	5.532366015308754
>5k	0.0	0.0
>10k+	0.002254822821405356	12.79933634007321
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20318	3.958362962284699	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18060	3.5184582684743413	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16712	3.2558402316026127	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10552	2.0557459384795815	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3294	0.641738734017413	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2974	0.5793961733356972	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2478	0.4827652042790375	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2404	0.4683484871213907	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2007	0.3910047477756369	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1925	0.3750294666009472	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1860	0.36236613396247364	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1663	0.3239864950427923	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1461	0.28463275361245916	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1431	0.2787881385485483	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1342	0.2614491138589461	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	1195	0.23281050004578283	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1113	0.2168352188710931	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1111	0.21644557786683238	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1102	0.21469219334765913	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1013	0.1973531686580569	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	965	0.1880017845557995	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	953	0.18566393853023516	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	951	0.18527429752597444	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	910	0.1772866569386296	TruSeq Adapter, Index 6 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	869	0.16929901635128475	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	830	0.16170101676820062	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	791	0.1541030171851165	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	756	0.1472842996105538	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	717	0.13968630002746968	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	661	0.1287763519081694	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	642	0.12507476236769252	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	635	0.12371101885277999	No Hit
ACGCAGAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAA	634	0.12351619835064963	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	629	0.12254209583999781	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	614	0.11961978830804239	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	612	0.11923014730378166	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	590	0.11494409625691368	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	585	0.11396999374626189	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	539	0.10500825064826522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.792820085214488E-4	0.0
2	0.0	0.0	0.0	0.008377281591605574	0.0
3	0.0	0.0	0.0	0.012273691634212817	0.0
4	0.0	0.0	0.0	0.019482050213036218	0.0
5	0.0	0.0	0.0	0.020845793727948755	0.0
6	0.0	0.0	0.0	0.021235434732209478	0.0
7	0.0	0.0	0.0	0.024352562766295275	0.0
8	0.0	0.0	0.0	0.02591112678333817	0.0
9	0.0	0.0	0.0	0.04091230544737606	0.0
10	0.0	0.0	0.0	0.05552384310715323	0.0
11	1.948205021303622E-4	0.0	0.0	0.06214774017958554	0.0
12	1.948205021303622E-4	0.0	0.0	0.07071984227332148	0.0
13	1.948205021303622E-4	0.0	0.0	0.07578517532871089	0.0
14	1.948205021303622E-4	0.0	0.0	0.08260389290327357	0.0
15	1.948205021303622E-4	0.0	0.0	0.09818953307370254	0.0
16	1.948205021303622E-4	0.0	0.0	0.13462096697208029	0.0
17	1.948205021303622E-4	0.0	0.0	0.1776762979428903	0.0
18	1.948205021303622E-4	0.0	0.0	0.19969101468362124	0.0
19	1.948205021303622E-4	0.0	0.0	0.23358978205430428	0.0
20	1.948205021303622E-4	0.0	0.0	0.2600853703440335	0.0
21	1.948205021303622E-4	0.0	0.0	0.32340203353640123	0.0
22	1.948205021303622E-4	0.0	0.0	0.42529315615058066	1.948205021303622E-4
23	1.948205021303622E-4	0.0	0.0	0.6113467356850766	1.948205021303622E-4
24	1.948205021303622E-4	0.0	0.0	0.8509759533054221	1.948205021303622E-4
25	1.948205021303622E-4	0.0	0.0	0.9059153349061841	1.948205021303622E-4
26	1.948205021303622E-4	0.0	0.0	0.9978706119117151	1.948205021303622E-4
27	1.948205021303622E-4	0.0	0.0	1.0658629671552116	1.948205021303622E-4
28	1.948205021303622E-4	0.0	0.0	1.1367776299306633	3.896410042607244E-4
29	1.948205021303622E-4	0.0	0.0	1.229122547940455	3.896410042607244E-4
30	1.948205021303622E-4	0.0	0.0	1.3993956668023917	3.896410042607244E-4
31	1.948205021303622E-4	0.0	0.0	1.930476355609759	3.896410042607244E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCAG	25	0.0054935445	29.6	3
TACTGTG	25	0.0054935445	29.6	7
TATGTCG	25	0.0054935445	29.6	5
TATCGGT	25	0.0054935445	29.6	13
AAAATGC	25	0.0054935445	29.6	26
ACACTGC	40	5.9338236E-5	27.75	6
TATACCA	65	2.6780453E-6	22.76923	5
CCGTCTT	180	0.0	22.611113	37
GCCGTCT	180	0.0	22.611113	36
TACACTG	50	2.6992796E-4	22.2	5
GCGTTAT	60	3.7211168E-5	21.583332	1
GAACAGA	60	3.7211168E-5	21.583332	1
AGCGTCA	95	7.1213435E-9	21.421053	3
ATGCCCC	115	1.3460522E-10	20.913044	37
ATACACT	80	6.944683E-7	20.8125	4
GATACAC	90	9.45256E-8	20.555557	3
ACAGAGG	45	0.0038229658	20.555557	3
ATACAGG	45	0.0038229658	20.555557	1
TCTATGA	45	0.0038229658	20.555557	2
TATGCCG	230	0.0	19.304348	33
>>END_MODULE