FastQCFastQC Report
Fri 10 Feb 2017
ERR1630544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910421
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74110.38792496523017705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68850.36039176705029935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53460.27983360735670304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39970.20922089947713096No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG24720.12939556254877851No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT23140.12112513419816888No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG22280.11662350864024212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1052.2617314E-819.380955
AATACCG609.2398666E-418.55
TCTGTCG650.001580451817.0769238
CGCTAAT1757.2759576E-1216.91428632
ATACCGT1757.2759576E-1216.9142866
TCCGCTA700.002593336615.85714228
TCGCCAT5850.015.81196513
ATCTCGT2003.6379788E-1215.72537
GGTATCA38300.015.3603141
TAACGGC855.36646E-415.23529436
TACAGCG855.36646E-415.2352947
GTTATAC1101.4527894E-515.1363653
TTATACT2450.015.1020414
CGTATAT750.004105727714.810
CGAATTA1752.240995E-914.815
TCTTATA19800.014.76262737
GTCGCCA6450.014.62790712
ACCGTCG1904.5656634E-1014.6052648
TCTAATA2301.8189894E-1214.4782612
TAGAACA9250.014.44
Produced by FastQC (version 0.11.5)