##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630544.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1910421
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.011424706910155	33.0	31.0	34.0	30.0	34.0
2	32.1896995479007	33.0	31.0	34.0	30.0	34.0
3	32.290042351921386	34.0	31.0	34.0	30.0	34.0
4	35.88557495965549	37.0	35.0	37.0	35.0	37.0
5	35.83786662730362	37.0	35.0	37.0	35.0	37.0
6	35.91655870617	37.0	35.0	37.0	35.0	37.0
7	35.862651740113826	37.0	35.0	37.0	35.0	37.0
8	35.86513443895351	37.0	35.0	37.0	35.0	37.0
9	37.57482774739181	39.0	37.0	39.0	35.0	39.0
10	37.47751935306406	39.0	37.0	39.0	35.0	39.0
11	37.573137544028256	39.0	37.0	39.0	35.0	39.0
12	37.519974393078805	39.0	37.0	39.0	35.0	39.0
13	37.55500332125746	39.0	37.0	39.0	35.0	39.0
14	38.84915105099871	40.0	38.0	41.0	35.0	41.0
15	38.86687803368996	40.0	38.0	41.0	35.0	41.0
16	38.823101818918445	40.0	38.0	41.0	35.0	41.0
17	38.80324336887	40.0	38.0	41.0	35.0	41.0
18	38.78799960846327	40.0	38.0	41.0	35.0	41.0
19	38.865933215767626	40.0	38.0	41.0	35.0	41.0
20	38.83218358675915	40.0	38.0	41.0	35.0	41.0
21	38.790087629899375	40.0	38.0	41.0	35.0	41.0
22	38.72803219813853	40.0	38.0	41.0	35.0	41.0
23	38.67122430082165	40.0	38.0	41.0	34.0	41.0
24	38.6253564005002	40.0	38.0	41.0	34.0	41.0
25	38.58021347127151	40.0	38.0	41.0	34.0	41.0
26	38.439487421882404	40.0	38.0	41.0	34.0	41.0
27	38.31453538251516	40.0	38.0	41.0	34.0	41.0
28	38.22706618070048	40.0	38.0	41.0	34.0	41.0
29	38.16194702633608	40.0	38.0	41.0	33.0	41.0
30	38.07309697705375	40.0	38.0	41.0	33.0	41.0
31	38.02788547655203	40.0	37.0	41.0	33.0	41.0
32	37.9204070725772	40.0	37.0	41.0	33.0	41.0
33	37.84533880228494	40.0	37.0	41.0	33.0	41.0
34	37.780700693721435	40.0	37.0	41.0	33.0	41.0
35	37.72083587858383	40.0	37.0	41.0	33.0	41.0
36	37.67584108424269	40.0	37.0	41.0	33.0	41.0
37	37.54233857353955	40.0	37.0	41.0	33.0	41.0
38	37.39605825103472	40.0	37.0	41.0	32.0	41.0
39	37.34285113071935	40.0	36.0	41.0	32.0	41.0
40	37.17123607833038	40.0	36.0	41.0	31.0	41.0
41	37.1161984714364	40.0	36.0	41.0	31.0	41.0
42	36.98524618395631	40.0	36.0	41.0	31.0	41.0
43	36.200949947681686	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	5.0
12	4.0
13	2.0
14	4.0
15	3.0
16	12.0
17	26.0
18	58.0
19	129.0
20	246.0
21	486.0
22	871.0
23	1575.0
24	2753.0
25	4252.0
26	6564.0
27	9832.0
28	14051.0
29	19975.0
30	26804.0
31	34952.0
32	45021.0
33	58350.0
34	76563.0
35	102677.0
36	145895.0
37	229354.0
38	437967.0
39	691982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.54714432054505	18.865422857056117	11.779393128530307	24.808039693868526
2	18.27047546064454	20.79740538865517	36.23410756058482	24.698011590115478
3	20.04924568982439	23.791719207441712	29.79343296582272	26.365602136911182
4	14.100818615373262	15.232244620426597	34.48716277720984	36.179773986990305
5	13.614538366150708	38.15384148310765	34.71449486788514	13.517125282856501
6	35.02625861001319	34.63969460134703	16.08142917189457	14.252617616745209
7	28.79574711542639	30.899053140642824	21.682759978036255	18.622439765894534
8	26.73735265682276	34.51658037678606	20.04620970979695	18.69985725659423
9	26.467255123347154	13.431908464155285	19.784958393987502	40.315878018510055
10	15.868125402725367	27.74147687865659	33.87342371131808	22.51697400729996
11	34.787253699577214	21.762480625998144	22.425842261993562	21.02442341243108
12	21.015577194764923	25.961031626013327	29.856560412600153	23.166830766621597
13	29.559505470260223	20.22093559482439	26.297606653193196	23.9219522817222
14	22.513309893473743	21.302477307357908	26.25342790934564	29.930784889822714
15	25.48799453104839	28.283870413903532	23.243096678690193	22.98503837635788
16	24.878757090714558	27.082093423386784	24.530142832391395	23.509006653507264
17	23.267070452010316	27.205207647947756	26.116808808110882	23.410913091931047
18	22.382186962978317	25.702763945748085	28.026963690202315	23.888085401071283
19	23.491523596107875	26.259395180434055	27.771627300998052	22.477453922460022
20	24.056791670527073	25.184815284170348	28.020315940831892	22.73807710447069
21	23.898554297717624	26.01222453061393	26.695738792653557	23.393482379014888
22	23.911221662659695	25.572478526984366	26.829478947310566	23.686820863045373
23	23.070621606441723	26.157480471581916	27.166263352423364	23.605634569552993
24	23.869450765040796	26.267142163952347	26.948405613213005	22.915001457793856
25	23.91718893374811	25.603675838990466	27.03974673645233	23.439388490809094
26	23.555593243583484	26.294151917299903	26.96400426921605	23.18625056990056
27	23.881175929284698	25.71773446795235	26.587856812713007	23.813232790049945
28	22.993465838158187	26.072787097713018	27.379933532975194	23.553813531153605
29	22.790002831836542	26.649099858094104	27.35742540518556	23.203471904883795
30	22.57324432677405	27.294821403240437	27.193273105770928	22.938661164214587
31	23.34317933062922	26.552419597565148	27.001273541277026	23.10312753052861
32	22.38014552813228	26.51640659310173	27.246088689351716	23.85735918941427
33	22.794923213260322	26.098069483113935	27.468657432052936	23.63834987157281
34	23.238123952783184	26.02447313969015	27.164588328959955	23.572814578566714
35	23.285966810456962	26.14298104972674	27.409874577383732	23.161177562432574
36	22.766814225764897	26.48112641140356	27.22169616016574	23.5303632026658
37	23.532875737860923	25.392727571566688	27.165739907591046	23.908656782981343
38	22.772938530303005	25.66020788088071	28.124324429013292	23.442529159802998
39	22.89914107937465	25.447584589993514	27.967081601385246	23.686192729246592
40	22.350256828206977	25.16031806601791	28.7889946770895	23.700430428685614
41	21.67454189416888	25.64298654589747	29.11410626244163	23.56836529749202
42	22.494989324342647	25.077404404578886	28.72188904958645	23.705717221492016
43	21.604871386987476	25.108287649685597	28.791036111935536	24.495804851391394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	104.0
1	143.5
2	183.0
3	810.5
4	1438.0
5	1438.0
6	1955.0
7	2472.0
8	2498.0
9	2524.0
10	3644.5
11	4765.0
12	4765.0
13	7723.5
14	10682.0
15	15204.5
16	19727.0
17	19143.0
18	18559.0
19	18559.0
20	21052.5
21	23546.0
22	23736.0
23	23926.0
24	26584.0
25	29242.0
26	29242.0
27	33109.0
28	36976.0
29	45905.5
30	54835.0
31	59011.5
32	63188.0
33	63188.0
34	68698.0
35	74208.0
36	79228.0
37	84248.0
38	95658.0
39	107068.0
40	107068.0
41	112235.0
42	117402.0
43	113934.0
44	110466.0
45	116254.0
46	122042.0
47	122042.0
48	126514.0
49	130986.0
50	135253.0
51	139520.0
52	143986.0
53	148452.0
54	148452.0
55	134532.5
56	120613.0
57	113833.5
58	107054.0
59	97657.5
60	88261.0
61	88261.0
62	82532.5
63	76804.0
64	67950.0
65	59096.0
66	51572.0
67	44048.0
68	44048.0
69	37799.0
70	31550.0
71	27274.5
72	22999.0
73	18462.0
74	13925.0
75	13925.0
76	11004.5
77	8084.0
78	6580.0
79	5076.0
80	4003.5
81	2931.0
82	2931.0
83	2164.0
84	1397.0
85	1149.5
86	902.0
87	803.0
88	704.0
89	704.0
90	532.0
91	360.0
92	199.0
93	38.0
94	27.0
95	16.0
96	16.0
97	10.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1910421.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.79556509957452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.58870860085554	47.75470362527548
2	8.924524970136021	9.958978279080055
3	2.286113445588659	3.82664974735064
4	0.9399987012277579	2.0979103491147546
5	0.49451921379688674	1.3795989493197303
6	0.30564204423922575	1.0232082345910054
7	0.2114735717194389	0.8259501206398046
8	0.14841632079281541	0.6624777990907893
9	0.11617512197897426	0.5833850923195981
>10	0.7583975877481367	8.304478355419858
>50	0.10120654376736954	3.9850757447724905
>100	0.11318679940286211	13.074501307224704
>500	0.008446266832169284	3.1665290926853564
>1k	0.002909269686636087	2.328045300560431
>5k	2.8154222773897614E-4	1.0285080025552853
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7411	0.38792496523017705	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6885	0.36039176705029935	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5346	0.27983360735670304	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3997	0.20922089947713096	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2472	0.12939556254877851	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2314	0.12112513419816888	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2228	0.11662350864024212	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.00120392311432925	0.0
3	0.0	0.0	0.0	0.0018320569131097281	0.0
4	0.0	0.0	0.0	0.0030883245106706845	0.0
5	0.0	0.0	0.0	0.003559424859756043	0.0
6	0.0	0.0	0.0	0.003716458309451163	0.0
7	0.0	0.0	0.0	0.004082869692073108	0.0
8	0.0	0.0	0.0	0.004239903141768228	0.0
9	0.0	0.0	0.0	0.007118849719512086	0.0
10	0.0	0.0	0.0	0.01067827457926813	0.0
11	0.0	0.0	0.0	0.012039231143292499	0.0
12	0.0	0.0	0.0	0.013347843224085162	0.0
13	0.0	0.0	0.0	0.01397597702286564	0.0
14	0.0	0.0	0.0	0.01460411082164612	0.0
15	0.0	0.0	0.0	0.015860378419207077	0.0
16	0.0	0.0	0.0	0.018844013963414348	0.0
17	0.0	5.234448323170652E-5	0.0	0.02182764950762162	0.0
18	0.0	5.234448323170652E-5	0.0	0.024287840219511823	0.0
19	0.0	5.234448323170652E-5	0.0	0.02690506438109715	0.0
20	0.0	5.234448323170652E-5	0.0	0.02946994405945077	0.0
21	0.0	1.0468896646341304E-4	0.0	0.03402391410060924	0.0
22	0.0	1.0468896646341304E-4	0.0	0.04245137590091399	0.0
23	0.0	1.0468896646341304E-4	0.0	0.05794534293749912	0.0
24	0.0	1.0468896646341304E-4	0.0	0.08249490557316948	0.0
25	0.0	1.0468896646341304E-4	0.0	0.08778169837957184	0.0
26	0.0	1.0468896646341304E-4	0.0	0.0979888726097546	0.0
27	0.0	1.0468896646341304E-4	0.0	0.11023748168597393	0.0
28	0.0	1.0468896646341304E-4	0.0	0.13698551261737596	0.0
29	0.0	2.0937793292682608E-4	0.0	0.1862416713384118	0.0
30	0.0	2.0937793292682608E-4	0.0	0.2521433757271303	0.0
31	5.234448323170652E-5	2.0937793292682608E-4	0.0	0.5340184179298699	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	105	2.2617314E-8	19.38095	5
AATACCG	60	9.2398666E-4	18.5	5
TCTGTCG	65	0.0015804518	17.076923	8
CGCTAAT	175	7.2759576E-12	16.914286	32
ATACCGT	175	7.2759576E-12	16.914286	6
TCCGCTA	70	0.0025933366	15.857142	28
TCGCCAT	585	0.0	15.811965	13
ATCTCGT	200	3.6379788E-12	15.725	37
GGTATCA	3830	0.0	15.360314	1
TAACGGC	85	5.36646E-4	15.235294	36
TACAGCG	85	5.36646E-4	15.235294	7
GTTATAC	110	1.4527894E-5	15.136365	3
TTATACT	245	0.0	15.102041	4
CGTATAT	75	0.0041057277	14.8	10
CGAATTA	175	2.240995E-9	14.8	15
TCTTATA	1980	0.0	14.762627	37
GTCGCCA	645	0.0	14.627907	12
ACCGTCG	190	4.5656634E-10	14.605264	8
TCTAATA	230	1.8189894E-12	14.478261	2
TAGAACA	925	0.0	14.4	4
>>END_MODULE