FastQCFastQC Report
Fri 10 Feb 2017
ERR1630543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137397
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62210.29105496077705734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54990.2572755552665228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47640.22288793331327778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34350.1607094985161858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29200.020.0839041
TAGGACG650.001580534917.0769234
ACTGCGT957.064216E-515.5789488
ACGGACC3150.015.2698418
CGATTAA1101.4529509E-515.13636424
GACGGAC3200.015.031257
CGAACGA2109.094947E-1214.976191516
AAGACGG3000.014.7999995
ACGAACG2151.2732926E-1114.62790715
TTAACGG1406.001301E-714.53571535
GCATTCG2450.014.34693822
TAACGAA2201.8189894E-1114.29545413
TCTTATA17900.014.2625737
CCGATAA2600.014.2307699
GCGTTAT3000.014.1833321
TGAACGG1051.6566817E-414.0952395
ATACCGT2650.013.9622646
CGCAAGA3450.013.9420292
GTCCTAT3350.013.805971
CTTATAC11950.013.77824337
Produced by FastQC (version 0.11.5)