##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630543.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2137397
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08864801438385	33.0	31.0	34.0	30.0	34.0
2	32.269315433679374	34.0	31.0	34.0	30.0	34.0
3	32.37828255583778	34.0	31.0	34.0	30.0	34.0
4	35.9440571873171	37.0	35.0	37.0	35.0	37.0
5	35.89544291491005	37.0	35.0	37.0	35.0	37.0
6	35.97008323675948	37.0	35.0	37.0	35.0	37.0
7	35.92005462719373	37.0	35.0	37.0	35.0	37.0
8	35.92133281744103	37.0	35.0	37.0	35.0	37.0
9	37.635855201443626	39.0	37.0	39.0	35.0	39.0
10	37.549381326913064	39.0	37.0	39.0	35.0	39.0
11	37.63943432127957	39.0	37.0	39.0	35.0	39.0
12	37.586701019978975	39.0	37.0	39.0	35.0	39.0
13	37.62584910524343	39.0	37.0	39.0	35.0	39.0
14	38.94036484565104	40.0	38.0	41.0	36.0	41.0
15	38.95409463005703	40.0	38.0	41.0	36.0	41.0
16	38.920646000719564	40.0	38.0	41.0	36.0	41.0
17	38.90313732076914	40.0	38.0	41.0	35.0	41.0
18	38.88642540435867	40.0	38.0	41.0	35.0	41.0
19	38.945197827076576	40.0	38.0	41.0	35.0	41.0
20	38.91076107994912	40.0	38.0	41.0	35.0	41.0
21	38.87190540643596	40.0	38.0	41.0	35.0	41.0
22	38.81054899955413	40.0	38.0	41.0	35.0	41.0
23	38.75870790498911	40.0	38.0	41.0	35.0	41.0
24	38.7118532495367	40.0	38.0	41.0	34.0	41.0
25	38.66614765530222	40.0	38.0	41.0	34.0	41.0
26	38.54395463266768	40.0	38.0	41.0	34.0	41.0
27	38.42641586939628	40.0	38.0	41.0	34.0	41.0
28	38.33584776248867	40.0	38.0	41.0	34.0	41.0
29	38.273884074881735	40.0	38.0	41.0	34.0	41.0
30	38.202814919268626	40.0	38.0	41.0	34.0	41.0
31	38.16993988482252	40.0	38.0	41.0	34.0	41.0
32	38.08038562793903	40.0	38.0	41.0	33.0	41.0
33	38.00611070381403	40.0	37.0	41.0	33.0	41.0
34	37.955529553003025	40.0	37.0	41.0	33.0	41.0
35	37.90847699327734	40.0	37.0	41.0	33.0	41.0
36	37.87654001572941	40.0	37.0	41.0	33.0	41.0
37	37.75901294892807	40.0	37.0	41.0	33.0	41.0
38	37.6251014668777	40.0	37.0	41.0	33.0	41.0
39	37.579615298421395	40.0	37.0	41.0	33.0	41.0
40	37.42724164018196	40.0	37.0	41.0	32.0	41.0
41	37.38222099123373	40.0	36.0	41.0	32.0	41.0
42	37.2607484711544	40.0	36.0	41.0	31.0	41.0
43	36.485867155236015	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	5.0
11	2.0
12	1.0
13	2.0
14	1.0
15	4.0
16	7.0
17	27.0
18	35.0
19	118.0
20	219.0
21	440.0
22	817.0
23	1466.0
24	2451.0
25	4040.0
26	6362.0
27	9330.0
28	13918.0
29	19781.0
30	26988.0
31	36129.0
32	47321.0
33	62100.0
34	83010.0
35	111861.0
36	160254.0
37	250578.0
38	478153.0
39	821970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.271418458994745	19.244436106160904	12.099904697162016	25.38424073768233
2	18.68319268717978	21.015234886172294	34.93291138707503	25.3686610395729
3	20.274801545992627	23.539567052821727	29.479689547613287	26.705941853572355
4	14.462825577092136	15.567393422934533	34.78113799167866	35.18864300829467
5	14.330889394904176	37.041691365712595	34.34902360207299	14.278395637310243
6	34.68737908774084	34.815806328913155	15.866074482185574	14.63074010116043
7	28.77696562688167	30.527646478403405	21.869451487019024	18.825936407695902
8	27.041115899386032	33.48792947683561	20.257443984435273	19.21351063934309
9	26.674408170311832	13.977749571090442	20.120876000106673	39.22696625849105
10	16.93892150124661	26.804426131411248	33.316272082350636	22.94038028499151
11	35.302847341883606	21.85246821250334	21.751270353612362	21.09341409200069
12	21.58358975894511	25.618544425766483	29.314161103435627	23.48370471185278
13	29.80414962685921	20.134537477127555	25.47668963697432	24.584623259038914
14	22.76643038237632	21.121532406005997	25.574939985412165	30.537097226205518
15	25.510609400125478	27.808685050086623	22.859300354590186	23.82140519519771
16	25.076249288269796	26.57559639131149	24.362016041006886	23.986138279411826
17	23.409736235243148	26.573771741983354	25.910722247668545	24.105769775104953
18	23.245985654513408	25.38531681292713	27.262506684532635	24.106190848026827
19	23.92442770341682	25.660324216792667	27.178432457797967	23.236815621992545
20	24.462558897574947	25.02146302254565	27.011313293693217	23.50466478618619
21	24.66411246951315	25.508597607276513	26.269757092388545	23.55753283082179
22	24.507239413174062	25.31307941388521	26.44417485380582	23.73550631913491
23	23.94955172108878	25.49414077029209	26.647553075072157	23.90875443354697
24	24.063756054677725	25.728210528975197	26.41741333032656	23.79062008602052
25	24.293661870022273	25.365058526796847	26.562262415452064	23.77901718772881
26	23.98225505135452	25.96452600990831	26.36417099864929	23.689047940087875
27	24.270362501678445	25.41062797411992	26.24907773333639	24.069931790865244
28	23.58420078254063	25.625000877235255	26.87231244359377	23.91848589663034
29	23.358692839935678	25.965134226351022	26.953205230474264	23.722967703239036
30	23.284443648044796	26.418536191451565	26.83132801253113	23.46569214797251
31	23.86131355101556	26.165190650122554	26.226573724956104	23.746922073905782
32	23.34250492538354	25.747813812782557	26.562870631894775	24.346810629939128
33	23.237844911357133	25.68558859210526	26.958117747896154	24.118448748641455
34	23.86281069918223	25.42817267919811	26.78341927119763	23.92559735042203
35	23.75670032286936	25.603432586459135	27.137915885537407	23.501951205134095
36	23.330153453008496	26.03433054317939	26.710620441593207	23.924895562218904
37	23.855231386588454	25.07302106253541	26.961673474792004	24.110074076084135
38	23.170566815617313	25.305640458932054	27.43640980126762	24.08738292418301
39	23.274571827320802	25.18376324098892	27.527501910033557	24.014163021656714
40	22.851580684355785	25.10249616706676	28.05000662020205	23.995916528375403
41	22.471164692380498	25.281686088265303	28.250203401614204	23.99694581773999
42	22.859534283991227	25.12907054702519	28.10105001550952	23.910345153474065
43	22.289541905411113	24.6621942484246	28.27340919819762	24.774854647966663
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	245.0
1	241.0
2	237.0
3	724.0
4	1211.0
5	1211.0
6	1632.5
7	2054.0
8	1995.5
9	1937.0
10	2874.0
11	3811.0
12	3811.0
13	6418.0
14	9025.0
15	12236.5
16	15448.0
17	15749.5
18	16051.0
19	16051.0
20	19377.0
21	22703.0
22	23392.0
23	24081.0
24	28046.0
25	32011.0
26	32011.0
27	36740.0
28	41469.0
29	48997.0
30	56525.0
31	62982.5
32	69440.0
33	69440.0
34	77018.5
35	84597.0
36	91860.5
37	99124.0
38	108017.0
39	116910.0
40	116910.0
41	122618.5
42	128327.0
43	129256.5
44	130186.0
45	132651.0
46	135116.0
47	135116.0
48	138066.0
49	141016.0
50	142392.5
51	143769.0
52	146509.5
53	149250.0
54	149250.0
55	143185.0
56	137120.0
57	131920.0
58	126720.0
59	118488.0
60	110256.0
61	110256.0
62	102633.5
63	95011.0
64	84177.0
65	73343.0
66	64944.5
67	56546.0
68	56546.0
69	48914.5
70	41283.0
71	35685.5
72	30088.0
73	23868.5
74	17649.0
75	17649.0
76	13799.0
77	9949.0
78	8104.0
79	6259.0
80	5018.0
81	3777.0
82	3777.0
83	2896.5
84	2016.0
85	1689.5
86	1363.0
87	1148.0
88	933.0
89	933.0
90	693.5
91	454.0
92	262.5
93	71.0
94	41.5
95	12.0
96	12.0
97	8.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2137397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.809078002066116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.51693241215929	52.002058135484106
2	8.79841440988723	10.7004693629067
3	2.3209183776253877	4.233987199843528
4	0.996544218633889	2.4239574049366475
5	0.541615906976356	1.6467581967242506
6	0.357148380138334	1.303071822368611
7	0.23085121064673744	0.9826494492562198
8	0.1728311802066957	0.8407763774702484
9	0.12642135482557643	0.691880942404382
>10	0.7559003387442123	8.915524119761072
>50	0.09717422826096225	4.13043618388605
>100	0.08022153137715096	9.256507496849235
>500	0.004485140462438992	1.7694534852938075
>1k	3.8665003986542855E-4	0.5513524705697683
>5k	1.5466001594617143E-4	0.5511173522454316
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6221	0.29105496077705734	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5499	0.2572755552665228	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4764	0.22288793331327778	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3435	0.1607094985161858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.403576406254898E-4	0.0
2	0.0	0.0	0.0	9.357176041699319E-4	0.0
3	0.0	0.0	0.0	0.0016375058072973809	0.0
4	0.0	0.0	0.0	0.0025264375312588163	0.0
5	0.0	0.0	0.0	0.0030878680937607753	0.0
6	0.0	0.0	0.0	0.003134653973969272	0.0
7	0.0	0.0	0.0	0.0034621551354287483	0.0
8	0.0	0.0	0.0	0.0036492986562627347	0.0
9	0.0	4.67858802084966E-5	0.0	0.0062225220677300476	0.0
10	0.0	9.35717604169932E-5	0.0	0.008748959598988864	0.0
11	0.0	9.35717604169932E-5	0.0	0.0096378913229503	0.0
12	0.0	9.35717604169932E-5	0.0	0.010292893645869251	0.0
13	0.0	9.35717604169932E-5	0.0	0.010947895968788203	0.0
14	0.0	9.35717604169932E-5	0.0	0.01169647005212415	0.0
15	0.0	9.35717604169932E-5	0.0	0.012866117057336564	0.0
16	0.0	9.35717604169932E-5	0.0	0.014877909906301917	4.67858802084966E-5
17	0.0	1.403576406254898E-4	0.0	0.016842916875058775	4.67858802084966E-5
18	0.0	1.403576406254898E-4	0.0	0.018012563880271188	4.67858802084966E-5
19	0.0	1.403576406254898E-4	0.0	0.01979042732819406	4.67858802084966E-5
20	0.0	1.403576406254898E-4	0.0	0.022223293099035882	4.67858802084966E-5
21	0.0	1.403576406254898E-4	0.0	0.025591876474047637	4.67858802084966E-5
22	0.0	1.403576406254898E-4	0.0	0.029334746890727365	4.67858802084966E-5
23	0.0	1.403576406254898E-4	0.0	0.03466833723449598	9.35717604169932E-5
24	0.0	1.403576406254898E-4	0.0	0.04163943338556197	9.35717604169932E-5
25	0.0	1.403576406254898E-4	0.0	0.044867659119948236	9.35717604169932E-5
26	0.0	1.403576406254898E-4	0.0	0.05076268002621881	9.35717604169932E-5
27	0.0	1.403576406254898E-4	0.0	0.06334808180230439	9.35717604169932E-5
28	0.0	1.871435208339864E-4	0.0	0.08617959134405073	1.403576406254898E-4
29	0.0	1.871435208339864E-4	0.0	0.12342115199001402	1.403576406254898E-4
30	0.0	1.871435208339864E-4	0.0	0.1828392198548047	1.403576406254898E-4
31	0.0	1.871435208339864E-4	0.0	0.3816792107409152	1.403576406254898E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2920	0.0	20.083904	1
TAGGACG	65	0.0015805349	17.076923	4
ACTGCGT	95	7.064216E-5	15.578948	8
ACGGACC	315	0.0	15.269841	8
CGATTAA	110	1.4529509E-5	15.136364	24
GACGGAC	320	0.0	15.03125	7
CGAACGA	210	9.094947E-12	14.9761915	16
AAGACGG	300	0.0	14.799999	5
ACGAACG	215	1.2732926E-11	14.627907	15
TTAACGG	140	6.001301E-7	14.535715	35
GCATTCG	245	0.0	14.346938	22
TAACGAA	220	1.8189894E-11	14.295454	13
TCTTATA	1790	0.0	14.26257	37
CCGATAA	260	0.0	14.230769	9
GCGTTAT	300	0.0	14.183332	1
TGAACGG	105	1.6566817E-4	14.095239	5
ATACCGT	265	0.0	13.962264	6
CGCAAGA	345	0.0	13.942029	2
GTCCTAT	335	0.0	13.80597	1
CTTATAC	1195	0.0	13.778243	37
>>END_MODULE