FastQCFastQC Report
Fri 10 Feb 2017
ERR1630542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007085
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111720.5566281447970565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108210.539140096209179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91380.45528714528781794No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76420.38075118891327475No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC51910.2586337897996348No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT37660.18763530194286737No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA34800.17338578087126355No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA34550.17214019336500447No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG31500.15694402578864372No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG28780.143392033720545No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG28140.14020332970452173No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG25860.12884357164743895No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG24120.12017428260387578No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG23630.11773293109160797No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.11768310759135761No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA22110.1101597590535528No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG21510.10717034903853101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA450.003826787520.55555713
GCTTTAT9450.019.1851841
TAACGGC1552.1827873E-1117.90322736
TTAACGG1554.0199666E-1016.70967935
GTCTTAT803.3841387E-416.18751
TAAGGGT1751.3278623E-1015.8571424
CGAGCTT1557.21775E-915.516129516
TCTATAC1455.351467E-815.3103453
GTACTAT2300.015.2826091
AGTCGGT3650.015.20548111
TATTGAG11350.015.158595
TTTATTG12800.014.8867193
GGTATCA66600.014.8333331
CGCCTTA3500.014.79999925
ACCTAGG16250.014.5723081
CTCTATG6900.014.478261
CTTTATT13350.014.4119852
TATACAG2353.6379788E-1214.1702145
GGGTGCG1051.6566031E-414.0952387
TATACTT2900.014.0344835
Produced by FastQC (version 0.11.5)