FastQCFastQC Report
Fri 10 Feb 2017
ERR1630541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764889
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105980.6004910223815776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT98660.5590153261763204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77350.4382711887263165No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54530.3089712724142991No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC19990.1132649135441379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG701.21966725E-418.55
TAGCGCA803.38385E-416.18754
TCTATAC1606.2937033E-1016.18753
CGAATTA1151.243041E-616.08695615
CGAACGA1302.5910595E-715.65384616
ACCTAGG7100.015.6338021
GTACTAG855.366172E-415.2352941
GGTATCA51800.015.1785721
TAGCCTA1101.4526659E-515.1363645
TCTTATA22700.015.07709237
ACGAACG1353.9753468E-715.07407315
GACCTAT1001.0938907E-414.86
AGTCGGT3300.014.57575711
GTAAACG1956.730261E-1014.2307727
CTCTATG7050.014.1702131
CTAGGCC8750.014.1657143
GTATCTA7950.013.96226326
CGGTCCA2009.840733E-1013.87500110
TCTATGG6700.013.805972
GCTTTAT7600.013.6315791
Produced by FastQC (version 0.11.5)